PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 21 | 3 |
Tytuł artykułu

Exposure to ticks and seroprevalence of Borrelia burgdorferi among a healthy young population living in the area of southern Podlasie, Poland

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Objectives. The objective of the study was assessment of risk of infection with Borrelia burgdorferi in the area of southern Podlasie in Poland, near the border with Belarus, by analysis of post-exposure procedure, and evaluation of asymptomatic infection in adolescents bitten by a tick, confirmed by serologic tests. Material and methods. The study was conducted among 128 healthy individuals aged 16–20 who declared being bitten by a tick. The level of IgM and IgG class antibodies was determined using the immunoenzymatic test (Borrelia 14 kD + OspC IgM ELISA and Borrelia IgG + VlsE ELISA, DRG Diagnostics). Positive and doubtful results were confirmed using the Western blot method (EUROLINE-WB, EUROIMMUN). Results. In the study group, the largest number of respondents (59.4%) declared tick bite in the region of the lower extremities, most often in the knee pit. Among the methods for removing the tick the largest number of respondents indicated removing it with the use of tweezers, with a simple, swift steady movement (29.7%), and pulling it out with the fingers (22.7%). In the ELISA test, a positive or doubtful result in at least one class was observed in 25.0% of respondents (n=32/128): in IgM class – 23.4% (n=30/128), and in IgG class – 4.7% (n=6/128). After verification with the Western blot test, infection was confirmed in 5.5% of respondents (n=7/128): in IgM class – 1.6% (n=2/128), in IgG class – 3.9% (n=5/128). In IgM class antibodies, the Western blot test confirmed positive or doubtful results of the ELISA test in 6.7%, while in IgG class antibodies in 83.3%. Conclusion. Evaluation of the actual infection with Borrelia spp. using serologic tests is difficult due to a certain non-specificity of the ELISA test, especially in IgM class antibodies, and difficulties with performance of a wide scope of specific Western blot tests. The variety of methods of tick removal declared by adolescents suggests that a wider education of society concerning appropriate methods of removing the tick should become an especially important element of prophylactic actions in the area of borreliosis.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
21
Numer
3
Opis fizyczny
p.512-517,fig.,ref.
Twórcy
autor
  • Department of Public Health, State School of Higher Education, Sidorska 95/97, 21-500 Biala Podlaska, Poland
  • Department of Public Health, State School of Higher Education, Biala Podlaska, Poland
  • Department of Public Health, State School of Higher Education, Biala Podlaska, Poland
Bibliografia
  • 1. Lindgren E, Jaenson T. Lyme borreliosis in Europe: influences of climate and climate change, epidemiology, ecology and adaptation measures.WHO, Regional Office for Europe 2006.
  • 2. Tylewska-Wierzbanowska S, Chmielewski T. Tick-borne zoonoses occurring in Poland. Post Mikrobiol. 2010; 49(3): 191–197 (in Polish).
  • 3. Website of National Institute of Public Health – National Institute of Hygiene http://www.pzh.gov.pl/oldpage/epimeld/index_p.html (access:2013.11.12).
  • 4. Stefanoff P, Rosińska M, Zieliński A. Epidemiology of tick-borne diseases in Poland. Przegl Epidemiol. 2006; 60(Suppl.1): 151–159 (in Polish).
  • 5. Derdakova M, Lencakova D. Association of genetic variability within the Borrelia burgdorferi sensu lato with the ecology, epidemiology ofLyme borreliosis in Europe. Ann Agric Environ Med. 2005; 12: 165–172.
  • 6. Zajkowska J. Lyme borreliosis – guidelines of treatment and expectations of patients. Przegl Epidemiol. 2008; 62(Suppl.1): 142–151 (in Polish).
  • 7. Chmielewski T, Tylewska-Wierzbanowska S. Prevalence of Borrelia burgdorferi antibodies in healthy population in Poland. Przegl Epidemiol. 2002; 56(1): 33–38 (in Polish).
  • 8. Bartosik K, Kubrak T, Olszewski T, Jung M, Buczek A. Prevention of tick bites and protection against tick-borne diseases in south-eastern Poland. Ann Agric Environ Med. 2008; 15: 181–185.
  • 9. Bartosik K, Sitarz M, Szymańska J, Buczek A. Tick bites on humans in the agricultural and recreational areas in south-eastern Poland. AnnAgric Environ Med. 2011; 18: 151–157.
  • 10. Steere AC, Coburn J, Glickstein L. The emergence of Lyme disease. J Clin Invest. 2004; 113(8): 1093–1101.
  • 11. Paluchowska E, Czarnecka I, Walory J, Zabielski S, Malewicz J, Samochocki Z, et al. Występowanie przeciwciał przeciwko Borreliaburgdorferi wśród żołnierzy odbywających służbę w północnowschodniejPolsce. Lekarz Wojskowy 2001; 77(1): 12–15 (in Polish).
  • 12. Walory J, Bukowska B, Grzesiowski P, Czarnecka I, Paluchowska E, Zabielski S, et al. Prevalence of antibodies against Anaplasma phagocytophilum, Babesia microti i Borrelia burgdorferi in adults in North-Eastern Poland. Pol Merkur Lekarski. 2005; XIX(114): 754–757 (in Polish).
  • 13. Cisak E, Chmielewska-Badora J, Zwoliński J, Dutkiewicz J, Patorska- Mach E. Incidence of tick-borne encephalitis virus and Borreliaburgdorferi infections in farmers of the Lublin province. Med Pr.2003; 54(2): 139–144 (in Polish).
  • 14. Zwoliński J, Chmielewska-Badora J, Cisak E, Buczek A, Dutkiewicz J. Prevalence of antibodies to Anaplasma phagocytophilum and Borrelia burgdorferi in forestry workers from the Lublin region. Wiad Parazytol.2004; 50(2): 221–227 (in Polish).
  • 15. Tomao P, Ciceroni L, D’Ovidio MC, De Rosa M, Vonesch N, Iavicoli S, et al. Prevalence and incidence of antibodies to Borrelia burgdorferi and o tick-borne encephalitis virus in agricultural and forestry workers from Tuscany, Italy. Eur J Clin Microbiol Infect Dis. 2005; 24(7): 457–463.
  • 16. Hristea A, Hristescu S, Ciufecu C, Vasile A. Seroprevalence of Borrelia burgdorferi in Romania. Eur J Epidemiol. 2001; 17(9): 891–896.
  • 17. Skogman BH, Ekerfelt Ch, Ludvigsson J, Forsberg P. Seroprevalence of Borrelia IgG antibodies among young Swedish children in relationto reported tick bites, symptoms and previous treatment for Lymeborreliosis: a population-based survey. Arch Dis Child. 2010; 95:1013–1016.
  • 18. Dehnert M, Fingerle V, Klier C, Talaska T, Schlaud M, Krause G, et al. Seropositivity of Lyme Borreliosis and Associated Risk Factors:A Population-Based Study in Children and Adolescents in Germany(KiGGS). PLoS ONE. 2012; 7(8): 41321. doi: 10.1371/journal.pone.0041321.
  • 19. Castiglia P, Mura I, Masia MD, Maida I, Solinas G, Muresu E. Prevalence of antibodies to Borrelia burgdorferi in Sardinian teen-agers. Ann Ig. 2004; 16(1–2): 103–108.
  • 20. Aslan Başbulut E, Gözalan A, Sönmez C, Cöplü N, Körhasan B, Esen B, et al. Seroprevalence of Borrelia burgdorferi and tick-borne encephalitis virus in a rural area of Samsun, Turkey. Mikrobiyol Bul. 2012; 46(2):247–256.
  • 21. Chmielewska-Badora J, Cisak E, Wójcik-Fatla A, Zwoliński J. Ocena korelacji testów serologicznych ELISA IgM/IgG i Westernblot IgM/ IgG w diagnostyce laboratoryjnej boreliozy. Przegl Epidemiol. 2006;60: 124 (in Polish).
  • 22. Ołdak E, Sulik A, Rożkiewicz D. The significance of immunoblot tests in diagnosis of Lyme borreliosis in children. Przegl Epidemiol. 2008; 62(Suppl.1): 83–87.
  • 23. Kaya AD, Parlak AH, Ozturk CE, Behcet M. Seroprevalence of Borrelia burgdorferi infection among forestry workers and farmers in Duzce,north-western Turkey. New Microbiol. 2008; 31: 203–209.
  • 24. Witecka-Knysz E, Klimczak M, Lakwa K, Zajkowska J, Pancewicz S, Kondrusik M, et al. Borelioza: dlaczego diagnostyka jest tak trudna?Diagnosta Laboratoryjny 2007; 1(13): 11–13 (in Polish).
  • 25. Zajkowska J, Kondrusik M, Pancewicz S, Grygorczuk S, Świerzbińska R, Hermanowska-Szpakowicz T, et al. Western-blot with VlsE protein and „in vivo” antigens in Lyme borreliosis diagnosis. Przegl Epidemiol. 2006; 60(Suppl.1): 177–185 (in Polish).
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-1ee94a5f-7398-4b30-88b3-5edac0a47f6d
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.