Relationship between extracellular matrix components and MMPs activity during development of neural stem cells from umbilical cord blood (HUCB-NSC)

• Autorzy: Szymczak P. , Sypecka J. , Zalewska T.
• Języki publikacji: EN

Abstrakty

EN

Accumulating evidence indicates that extracellular matrix components and metalloproteinases play a pivotal role in the development of stem cells. The aim of our study was to check if the presence of laminin, fi bronectin, and collagen affect MMPs activity (MMP-9 and MMP-2) which in turn might enhance cell proliferation and differentiation. The cells of HUCB-NSC were cultured without serum for two weeks on extracellular matrix components-coated plates. On 4th, 8th, and 14th day we performed proliferation assay, and determination of MMPs activity (in situ zymography) followed by immunocytochemistry with specifi c neural markers. Our results show that among all of the investigated ECM components fi bronectin stimulated most intensively cell proliferation and differentiation, especially toward neurons. We also observed the increase of MMPs activity (~20% increase in the 2-weeks culture) in the presence of fi bronectin. To confi rm the infl uence of MMPs on developmental processes of neural stem cells we used inhibitors of MMPs – GM6001, and doxycycline. We found that the inhibition of MMPs by GM6001 decreased cell proliferation (~30%) and differentiation into neurons (~20%). As it was shown in the present study the fi bronectin occured to be the potent factor in promoting cell proliferation and differentiation and support the idea that MMPs may contribute to the mechanism involved in the development of neural stem cells. Supported by MSRHE grants: 1266/P01/2006/31 and N40101832 /0296.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.293-294

Twórcy

autor

Szymczak P.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Sypecka J.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Zalewska T.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland