Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli

• Autorzy: Filipkowski P. , Pietrow O. , Panek A. , Synowiecki J.
• Języki publikacji: EN

Abstrakty

EN

 A trehalose synthase gene from Deinococcus radiodurans (DSMZ 20539) containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinant trehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography and characterized. The expressed enzyme is a homodimer with molecular mass of 126.9 kDa and exhibits the highest activity of 11.35 U/mg at pH 7.6 and at 30°C. DraTreS activity was almost unchanged after 2 h preincubation at 45°C and pH 7.6, and retained about 56% of maximal value after 8 h incubation at 50°C. The DraTreS was strongly inhibited by Cu2+, Hg2+, Zn2+, Al3+ and 10 mM Tris. The Km value of maltose conversion was 290.7 mM.

Słowa kluczowe

EN

recombinant trehalose; trehalose synthase; trehalose; Deinococcus radiodurans; gene expression; Escherichia coli

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 3
• Opis fizyczny: p.425-431,fig.,ref.

Twórcy

autor

Filipkowski P.

• Department of Food Chemistry, Technology and Biotechnology, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland

autor

Pietrow O.

autor

Panek A.

autor

Synowiecki J.

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