PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 72 | 2 |

Tytuł artykułu

Slowly-activating cation channel in the red beet vacuoles the presence of organolead: analysis of microscopic current

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Patch-clamp technique was used to examine effect of trimethyllead chloride (Met3PbCl) on the SV channel activity in the red beet (Beta vulgaris L.) taproot vacuoles. It was found that in the control bath macroscopic currents showed typical slow activation and strong outward rectification of the steady-state currents. The addition of Met3PbCl to the bath solution blocked in a concentration-dependent manner SV currents in red beet vacuoles. The time constant, τ, increased several times in the presence of 100 μM trimethyllead chloride at all voltages tested. Trimethyllead chloride decreased significantly (by about one order of magnitude) the open probability of single channels. The recordings of single channel activity obtained in the presence and in the absence of Met3PbCl showed that organolead only slightly (by ca. 10%) decreased the unitary conductance of single channels. It was also found that Met3PbCl diminished significantly the number of SV channel openings, whereas it did not change the opening times of the channels. Within the present work the existence of spatial correlation beetwen ion channels of Beta vulgaris vacuolar membrane was shown. Moreover, the comparison of data collected in the control and in the presence of trimethyllead shows that organolead compound disturb not only the ion transport through the membrane, but also spacial correlations among channels. This work was supported by the KBN grant N305 336434

Słowa kluczowe

Wydawca

-

Rocznik

Tom

72

Numer

2

Opis fizyczny

p.198

Twórcy

autor
  • Department of Physics and Biophysics, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland
  • Department of Physics and Biophysics, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland
autor
  • Department of Plant Physiology, Faculty Biology, University of Silesia, Katowice, Poland
autor
  • Department of Plant Physiology, Faculty Biology, University of Silesia, Katowice, Poland
  • Department of Physics and Biophysics, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland

Bibliografia

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-1e5a4f81-a5ad-41e7-86b4-b8f46dc6bf15
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.