Morphogenetic response of shoot tips to cryopreservation by encapsulation-dehydration in a solid mutant and periclinal chimeras of Chrysanthemum x grandiflorum /Ramat./Kitam

• Autorzy: Kulus D. , Abratowska A. , Mikula A.
• Języki publikacji: EN

Abstrakty

EN

Cryopreservation is widely applied to many economically important species excluding chimera plants which are problematic for long-term conservation. Their storage problems can be circumvented only by cryopreserving meristems. This study looked at the morphogenetic response of shoot tips of periclinal chimera chrysanthemum ‘Lady Orange’ and ‘Lady Salmon’, as well as the solid mutant ‘Richmond’, that were cryopreserved by encapsulation-dehydration technique. By applying 10 µM ABA in the preculture medium followed by 4-day-long dehydration treatment, the explant survival reached up to 67%. Besides the stimulation of typical single shoot recovery, cryopreservation led to direct or indirect multiple shoot formation, shoot malformation, as well as inhibited their spontaneous rooting. Microscopic analysis revealed three types of structural damages of shoot tips which can correspond with their morphogenetic response in recovery culture. No influence of cryostorage on the acclimatisation efficiency of the recovered chrysanthemums was observed.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2018
• Tom: 40
• Numer: 01
• Opis fizyczny: Article 18 [13p.], fig.,ref.

Twórcy

autor

Kulus D.

• Faculty of Agriculture and Biotechnology, University of Science and Technology, Poland, Bernardynska 6, 85‑029 Bydgoszcz, Poland

autor

Abratowska A.

• Laboratory of Ecotoxicology, Faculty of Biology, Institute of Botany, University of Warsaw, Miecznikowa 1, 02‑096 Warsaw, Poland

autor

Mikula A.

• Centre for Biological Diversity Conservation in Powsin, Polish Academy of Sciences Botanical Garden, Prawdziwka 2, 02‑973 Warsaw, Poland

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Identyfikatory

DOI

10.1007/s11738-017-2593-4