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2002 | 07 | 4 |
Tytuł artykułu

Natriuretic peptides reduce plasminogen activator inhibitor-1 expression in human endothelial cells

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Plasma concentrations of natriuretic peptides increase in some pathological conditions, but very little is known about the effect of these vasodilator peptides on the regulation of the blood coagulation system. The fundamental role in the regulation of fibrinolysis is played by plasminogen activator inhibitor type 1 (PAI-1). Recent studies demonstrate that natriuretic peptides can modulate PAI-1 expression in bovine aortic smooth muscle cells and rat aortic endothelial cells. In this report, we tested the effect of natriuretic peptides on PAI-1 expression in the human endothelial cell line (EA.hy 926). For this purpose, we treated the cell cultures with ANP, BNP and CNP, and modulation of PAI-1 synthesis was evaluated. We compared the effect of natriuretic peptides on synthesis and release of PAI-1 in unstimulated cells, and after activation with tumour necrosis factor α (TNFα). Natriuretic peptides abolished TNFα-induced upregulation of PAI-1 expression at both the PAI-1 mRNA and the antigen levels. The inhibitory efficiency was higher in the case of CNP when compared to that produced by ANP and BNP, particularly when TNFα-stimulated cells were used. We observed an inhibition of stimulatory effect of TNFα on PAI-1 expression also at the level of the PAI-1 promoter in cells transfected with a PAI-1 promoter fragment (+71 to -800) [1], The PAI-1 promoter activity was markedly inhibited by C-type natriuretic peptide, already at a very low (0.001 µM) concentration of the peptide.
Wydawca
-
Rocznik
Tom
07
Numer
4
Opis fizyczny
p.1153-1157,fig.,ref.
Twórcy
autor
  • Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland
  • Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland
autor
  • Department of Biochemistry, Medical University of Lodz, Lodz, Poland
autor
  • Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland
  • Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland
  • Department of Molecular and Medical Biophysics, Medical University of Lodz, Lodz, Poland
Bibliografia
  • 1. Świątkowska, M., Pawłowska, Z., Szemraj J. and Ciemiewski, C. S. The mechanism of the regulation of PAI-1 gene expression by reactive oxygen species. Cell. Mol. Biol. Lett. 7 (2002) Suppl. 160.
  • 2. Corti, R., Burnett, J. C., Rouleau, J. L., Ruschitzka, F. and Luscher, T. F. Vasopeptidase inhibitors. A new therapeutic concept in cardiovascular disease? Circulation, 104 (2001) 1856-1862.
  • 3. Aznar J. and Estelles A. Role of plasminogen activator inhibitor type 1 in the pathogenesis of coronary artery diseases. Haemostasis 24 (1994) 243-251.
  • 4. Rocha, E. and Paramo, J. A. The relationship between impaired fibrinolysis and coronary heart disease: a role for PAI-1. Fibrinolysis 8 (1994) 294-303.
  • 5. Yoshizumi, M., Tsuji, H., Nishimura H., Kasahara T., Sugano, T., Masuda, H., Nakagawa, K., Nakahara, Y., Kitamara, H., Yamada, K., Yoneda, M., Sawada, S., Nakagawa, M. Atrial natriuretic peptide inhibits the expression of tissue factor and plasminogen activator inhibitor 1 induced by angiotensin II in cultured rat aortic endothelial cells. Thromb. Haemost. 79 (1998) 631-634.
  • 6. Yoshizumi, M., Tsuji, H., Nishimura, H., Masuda, H., Kunieda, Y., Kawano, H., Kimura, S., Sugano, T., Kitamura, H., Nakagawa, K. and Nakagawa, M. Natriuretic peptides regulate the expression of tissue factor and PAI-1 in endothelial cells. Thromb. Haemost. 82 (1999) 1497-1503.
  • 7. Bouchie, J. L., Hansen, H. and Feener, E. P. Natriuretic factors and Nitric Oxide suppress plasminogen activator inhibitor -1 expression in vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 18 (1998) 1771-1779.
  • 8. Świątkowska, M., Ciemiewska-Cieślak, A., Pawłowska, Z. and Cierniewski C. S. Dual regulatory effects of nitric oxide on plasminogen activator inhibitor type 1 expression in endothelial cells. Eur. J. Biochem. 267 (2000) 1001-1007.
  • 9. Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate- phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159.
  • 10. Świątkowska, M., Pawłowska, Z., Szemraj, J., Drzewoski, J., Watala, C. and Ciemiewski, C. S. Cerivastatin, a HMG CoA reductase inhibitor, reduces plasminogen activator inhibitor -1 expression in endothelial cells by down-regulation of cellular signalling and the inhibition of PAI-1 promoter activity. Jap. J. Pharm. (in press).
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-1e05d04b-9a33-488b-b524-e2d131c5cb80
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