Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 54 | 2 |
Tytuł artykułu

Effect of exclusion of ambient solar UVA/B components on growth and antioxidant response of cotton ( Gossypium hirsutum L.)

Treść / Zawartość
Warianty tytułu
Języki publikacji
The influence of ambient solar UV-A or UV-B radiation on growth responses was investigated in three varieties of cotton (Gossypium hirsutum L.) after exclusion of solar UV-A/B radiation: JK-35, IH-63 and Khandwa-2. Cotton plants were grown from seeds in UV-exclusion chambers lined with selective UV filters to exclude either UV-B (280–315 nm) or UV-A/B (280–400 nm) from the solar spectrum under field conditions. Excluding UV-B and UV-A/B significantly increased plant height, leaf area and dry weight accumulation in all three varieties of cotton. The varieties differed considerably in their sensitivity to ambient UV-A/B. Khandwa-2 was most sensitive and JK-35 least sensitive to ambient solar UV. We monitored the activity of the antioxidant enzymes superoxide dismutase (SOD), ascorbic acid peroxidase (APX), glutathione reductase (GR) and guaiacol peroxidase (GPX), as well as the level of the antioxidant ascorbic acid (ASA), in primary leaves of the most UV-sensitive variety (Khandwa-2). The level of UV-B-absorbing substances was significantly decreased by exclusion of solar UV-B and UV-A/B. Exclusion of solar UV decreased the activity of all the antioxidant enzymes monitored and the level of ascorbic acid versus control plants (+UV-A/B) grown under filters transparent to solar UV. Reduction of the antioxidant defense after UV exclusion indicates that ambient solar UV exerts significant stress and induces some reactive oxygen species to accumulate, which in turn retards the growth and development of cotton plants. Ambient solar UV stresses cotton plants, shifting their metabolism towards defense against solar UV. Exclusion of solar UV eliminates the need for that defense and leads to enhancement of primary metabolism.
Słowa kluczowe
Opis fizyczny
  • School of Life Sciences, Devi Ahilya University, Khandwa Road Campus, Indore-452 001, India
  • AGRAWAL SB, and RATHORE D. 2007. Changes in oxidative stress defense system in wheat (Triticum aestivum L.)and mung bean (Vigna radiata L.) cultivars grown withand without mineral nutrients and irradiated by supplementalultraviolet-B. Environmental and Experimental Botany 59: 21–33.
  • ALBERT KR, MIKKELSEN TN, and RO-POULSEN H. 2005. Effects of ambient versus reduced UV-B radiation on high arcticSalix arctica assessed by measurements and calculationsof chlorophyll-fluorescence parameters from fluorescencetransients. Physiologia Plantarum 124:208–226.
  • AMUDHA P, JAYAKUMAR M, and KULANDAIVELU G. 2005. Impacts of Ambient solar UV (280–400 nm) radiation on threetropical Legumes. Journal of Plant Biology 48: 284–291.
  • ARAKAWA N, TSUTSUMI K, SANCEDA NG, KURATA T, and INAGAKI C. 1981. A rapid and sensitive method for determinationof ascorbic acid using 4, 7-Diphenyl-l, 10-bathophenanthroline.Agricultural Biological Chemistry 45:1289–1290.
  • BEAUCHAMP CO, and FRIDOVICH I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamidegels. Analytical Biochemistry 44: 276–187.
  • COLEMAN RS, and DAY TA. 2004. Response of cotton and sorghum to several levels of subambient solar UV-B radiation:a test of the saturation hypothesis. PhysiologiaPlantarum 122: 362–372.
  • FOYER CH, and HARBINSON J. 1994. Oxygen metabolism and regulation of photosynthetic electron transport. In: FoyerCH, Mullineaux PM [eds.], Causes of Photo-oxidativeStress and Amelioration of Defense System in Plants,1–42. CRS press Boca Raton FL.
  • GALATRO A, SIMONTACCHI M, and PUNTARULO S. 2001. Free radical generation and antioxidant content in chloroplasts from soybean leaves exposed to ultraviolet-B. Physiologia Plantarum 113: 564–570.
  • GAO W, ZHENG Y, SLUSSER JR and HEISLER GM. 2003. Impact of enhanced ultraviolet-B irradiance on cotton growth,development, yield, and qualities under field conditions. Agricultural and Forest Meteorology 120: 241–248.
  • GURUPRASAD K, BHATTACHARJEE S, KATARIA S, YADAV S, TIWARI A, BARONIYA S, RAJIV A, and MOHANTY P. 2007. Growth enhancement of soybean (Glycine max) upon exclusionof UV-B and UV-A components of solar radiation.Characterization of photosynthetic parameters in leaves.Photosynthetic Research 94: 299–306.
  • JAIN K, KATARIA S, and GURUPRASAD KN. 2004. Effect of UV-B radiation on antioxidant enzyme and its modulation bybenzoquinone and alpha-tocopherol in cucumber cotyledons.Current Science 87: 87–90.
  • KAKANI VG, REDDY KR, ZHAO D, and SAILAJA K. 2003. Field crop responses to ultraviolet B radiation: a review.Agricultural Forest Meteorology 120: 191–21.
  • KATARIA S, JAIN K, and GURUPRASAD KN. 2007. UV-B induced changes in antioxidant Enzymes and their isoforms incucumber (Cucumis sativus L.) cotyledons. IndianJournal of Biochemistry and Biophysics 44: 31–37.
  • KRIZEK DT, and CHALKER S. 2005. Ultraviolet radiation and terrestrial ecosystems. Photochemistry and Photobiology81: 1021–1025.
  • LIAKOURA V, BORNMAN JF, and KARABOURNIOTIS G. 2003. The ability of abaxial and adaxial epidermis of sun and shadeleaves to attenuate UV-A and UV-B radiation in relationto the UV absorbing capacity of the whole leaf methanolicextracts. Physiologia Plantarum 117: 33–43.
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, and RANDALL RJ. 1951. Protein measurements with the Folin phenol reagent.Journal of Biological Chemistry 193: 165–27.
  • MAEHLY AC.1955. Plant peroxidases. In: Colowick PS, Kaplan NO [eds.], Methods in Enzymology, 271–285. AcademicPress New York.
  • MAZZA CA, BATISTA D, ZIMA AM, SZWARBERG- BRACCHITTA M, GIORDANO CV, ACEVEDO A, SCOPEL AL, and BALLARE CL.1999. The effects of solar UV-B radiation on the growthand field of barley are accompanied by increased DNAdamage and antioxidant responses. Plant Cell andEnvironment 22: 61–70.
  • MAZZA CA, BOCCALANDRO H, GIORDANO CV, BATTISTA D, SCOPEL AL, and BAILAR CL. 2000. Functional significance andinduction by solar radiation of UV-absorbing sunscreens,in field grown soybean crops. Journal of PlantPhysiology 122:117–125.
  • MCKENZIE RL, BJORN LO, BAIS A, and ILYASD M. 2003. Changes in biologically active UV radiation reaching the earth'ssurface. Photochemistry and Photobiology Sciences 2:5–15.
  • NAKANO Y and ASADA K. 1987. Purification of ascorbate peroxidase in spinach chloroplasts: its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbateradical. Plant Cell and Physiology 28:131–140.
  • PAL M, ZAIDI PH, VOLETI SR, and RAJ A. 2006. Solar UV-B Exclusion effects on growth and photosynthetic characteristics of Wheat and Pea. Journal of New Seeds 8: 19–34.
  • RAO MV, and ORMROD DP. 1995. Impact of UV-B and O3 on the free radical scavenging in Arabidopsis thaliana genotypes differing in flavonoid biosynthesis. Photochemistry and Photobiology 62: 719–726.
  • RAO MV, PALIJYATH G, and ORMROD DP. 1996. Ultraviolet-B and ozone-induced Biochemical changes in antioxidant enzymes of Arabidopsis thaliana. Plant Physiology 110: 125–136.
  • SAILE-MARK M, and TEVINI M. 1997. Effects of solar UV-B radiation on growth, flowering and yield of central and southern European bush bean cultivars (Phaseolus vulgaris L.). Plant Ecology 128:115–125.
  • SHINE MB, GURUPRASAD KN, and ANAND A. 2011. Enhancement of germination, growth, and photosynthesis in Soybeanby pre-treatment of seeds with magnetic field.Bioelectromagnetics 32 (6): 474–484.
  • SAHOO A, SARKAR S, SINGH RP, KAFATOS M, and SUMMERS ME. 2005. Declining trend of total ozone column over the northern parts of India. International Journal of Remote Sensing 26: 3433–3440.
  • SHARMA PK, ANANT P, and SANKHALKAR S. 1996. Oxidative damage and changes in activities of antioxidant enzymes in wheat seedlings exposed to ultraviolet-B radiation. Current Science 75: 359–366.
  • WILLEKENS H, CAMP WV, MONTAGU MV, INZE D, LANGEBARTELS C, and SANDERMANN H JR. 1994. Ozone, sulfur dioxide, and ultraviolet B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L. Plant Physiology 106: 1007–1014.
  • WILSON KE, THOMPSON JE, HUNER NPA, and GREENBERG BM. 2001. Effect of ultraviolet-A exposure on ultraviolet-Binducedaccumulation of specific flavonoids in Brassicanapus. Photochemistry and Photobiology 73: 678–684.
  • XU C, NATARAJAN S, and SULLIVAN JH. 2008. Impact of solar ultraviolet-B radiation on the antioxidant defense systemin soybean lines differing in flavonoids content.Environmental and Experimental Botany 63: 39–48.
  • YAO Y, YANG Y, REN J, and LI C. 2006. UV-spectra dependence of seedling injury and photosynthetic pigment change in Cucumis sativus and Glycine max. Environmental and Experimental Botany 57: 160–167
rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.