Anti-inflammatory effects of glycyrrhizin on lipoteichoic acid and lipopolysaccharide-induced bovine mastitis

• Autorzy: Kurumisawa T. , Kazama K. , Gondaira S. , Higuchi H. , Eguchi A. , Onda K. , Roh S.G. , Kawai K.
• Języki publikacji: EN

Abstrakty

EN

Bovine mastitis is primarily treated with antimicrobial agents. Anti-inflammatory agents are also used to alleviate clinical symptoms or reduce antimicrobial use. Glycyrrhizin is an anti-inflammatory agent used in the treatment of bovine mastitis, but its effects are not fully understood. We therefore examined the anti-inflammatory effects of glycyrrhizin both in vivo and in vitro. We first tested whether glycyrrhizin exerts anti-inflammatory effects using MAC-T cells, an immortalized bovine mammary epithelial cell line. Glycyrrhizin decreased the expression of interleukin (IL)-1β mRNA in a concentration-dependent manner in MAC-T cells stimulated with lipoteichoic acid (LTA). We then investigated the effects of glycyrrhizin in bovine mammary epithelial cells (bMECs), which seem to retain more of the characteristics of actual mammary epithelial cells. Stimulation with LTA or lipopolysaccharide significantly increased cytokine mRNA expression in bMECs. Glycyrrhizin exhibited a slight inhibitory effect, but no significant difference was observed. The effect of glycyrrhizin on LTA-induced mastitis was examined in lactating cows. Quarters were divided into test and control areas (test quarter: n=8, control quarter: n=7). All quarters were stimulated with LTA at the start of the trial (0 h). In the test quarter group, glycyrrhizin was administered via intramammary infusion. The somatic cell count and relative gene expression of IL-1β and tumor necrosis factor–α were significantly lower in test quarters than control quarters. Both the in vitro and in vivo studies showed that glycyrrhizin reduces the expression of proinflammatory cytokine genes in response to LTA-induced inflammation and partially revealed the mechanism of the anti-inflammatory effect of glycyrrhizin on mastitis. Further investigations involving field cases of mastitis with bacterial infections are needed to demonstrate the anti-inflammatory effect of glycyrrhizin on bovine mastitis.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2025
• Tom: 28
• Numer: 1
• Opis fizyczny: p.35-42,fig.,ref.

Twórcy

autor

Kurumisawa T.

• School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe Chuo-ku, Sagamihara 252-5201, Japan
• Azabu University Mastitis Research Center, 1-17-71 Fuchinobe Chuo-ku, Sagamihara 252-5201, Japan

autor

Kazama K.

• School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe Chuo-ku, Sagamihara 252-5201, Japan

autor

Gondaira S.

• Graduate School of Veterinary Medicine, Rakuno Gakuen University, 852 Midorimachi Bunkyoudai, Ebetsu 069-8501, Japan

autor

Higuchi H.

• Graduate School of Veterinary Medicine, Rakuno Gakuen University, 852 Midorimachi Bunkyoudai, Ebetsu 069-8501, Japan

autor

Eguchi A.

• Graduate School of Veterinary Medicine, Rakuno Gakuen University, 852 Midorimachi Bunkyoudai, Ebetsu 069-8501, Japan

autor

Onda K.

• School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe Chuo-ku, Sagamihara 252-5201, Japan

autor

Roh S.G.

• Graduate School of Agricultural Science, Tohoku University, 468-1 Aramaki Aza Aoba, Aoba-ku, Sendai 980-0842, Japan

autor

Kawai K.

• School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe Chuo-ku, Sagamihara 252-5201, Japan
• Azabu University Mastitis Research Center, 1-17-71 Fuchinobe Chuo-ku, Sagamihara 252-5201, Japan

Identyfikatory

DOI

10.24425/pjvs.2025.154011