An improve protocol for PCR using LM1 and LM2 primers for Listeria monocytogenes detection in food matrices

• Autorzy: Godinez-Oviedo A. , Nava G.M. , Arvizu-Medrano S.M. , Hernandez-Iturriaga M.
• Języki publikacji: EN

Abstrakty

EN

Several studies have observed that use of a conventional PCR protocol with primers LM1 and LM2 for the identification ofthe hlyA geneofListeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason, in this study we provide a modified PCR protocol that improves the specificity of the results obtained with LM1 and LM2 primers.

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2017
• Tom: 66
• Numer: 2
• Opis fizyczny: p.255-257,fig.,ref.

Twórcy

autor

Godinez-Oviedo A.

• Microbial Food Safety Group, Food Research Department, Faculty of Chemistry, Universidad Autonoma de Queretaro, Queretaro, Mexico

autor

Nava G.M.

• Microbial Food Safety Group, Food Research Department, Faculty of Chemistry, Universidad Autonoma de Queretaro, Queretaro, Mexico

autor

Arvizu-Medrano S.M.

• Microbial Food Safety Group, Food Research Department, Faculty of Chemistry, Universidad Autonoma de Queretaro, Queretaro, Mexico

autor

Hernandez-Iturriaga M.

• Microbial Food Safety Group, Food Research Department, Faculty of Chemistry, Universidad Autonoma de Queretaro, Queretaro, Mexico

Bibliografia

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Identyfikatory

DOI

10.5604/01.3001.0010.7869