Bioflocculant production by a consortium of two bacterial species and its potential application in industrial wastewater and river water treatment

• Autorzy: Cosa S. , Okoh A.
• Języki publikacji: EN

Abstrakty

EN

We assessed the bioflocculant production potentials of a consortium of two marine bacterial species belonging to the Oceanobacillus and Halobacillus genera, isolated from sediment samples of Algoa Bay in the Eastern Cape Province of South Africa. Cell free culture broth of the consortium had a flocculating activity of 98.3%, which is higher than those of pure cultures of the individual species. The purified bioflocculant was more efficient (optimum dose 0.2 mg·ml⁻¹) in the flocculation of kaolin suspension (4 g·l⁻¹) compared to polyelectrolyte (optimum dose 0.3 mg·ml⁻¹) and alum (optimum dose 1 mg·ml⁻¹), which are both commercially available coagulants. A neutral pH condition and the presence of Ca²⁺ as cation resulted in optimum activity of the bioflocculant. Also, the purified bioflocculant removed chemical oxygen demand (COD) in brewery wastewater, dairy wastewater, and river water at efficiencies of 99.7, 99.9, and 63.5%, respectively, and also reduced their turbidity by 93.9, 88.3, and 98.6%, respectively. Composition analysis revealed the bioflocculant to be mainly polysaccharide with an amorphous-crystal-like structure. FTIR spectra revealed the presence of carboxyl, hydroxyl, and amino groups in its thermo-stability test, suggesting a thermostable bioflocculant.

Słowa kluczowe

EN

Oceanobacillus; Halobacillus; bioflocculant; production; bacterial species; application; industrial waste water; waste water treatment

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2014
• Tom: 23
• Numer: 3
• Opis fizyczny: p.689-696,fig.,ref.

Twórcy

autor

Cosa S.

• Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa

autor

Okoh A.

• Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa

Bibliografia

• 1. SARKAR B., CHAKRABARTI P.P., VIJAYKUMAR A., KALE V. Wastewater treatment in dairy industries – possibility of reuse. Desalination, 195, 141, 2006.
• 2. LIN J., HARICHUND C. Industrial effluent treatments using heavy-metal removing bacterial bioflocculants. Water SA. 37, 265, 2011.
• 3. TANIK A., GENCELI E.S., EKDAL A. Chemical treatability of dairy wastewater. J. Environ. Management and Health., 13, 63, 2002.
• 4. BUTHELEZI S.P., OLANIRAN A.O., PILLAY B. Turbidity and microbial load removal from river water using bioflocculants from indigenous bacteria isolated from wastewater in South Africa. African J. Biotechnol., 8, 3261, 2009.
• 5. GHOSH M., GANGULI A., PATHAK S. Application of a novel biopolymer of Salmonella from poultry wastewater. Environ. Technol., 30, 337, 2008.
• 6. ZHANG C., CUI Y., WANG Y. Bioflocculant produced from bacteria for decolorization, Cr removal and swine wastewater application. Sustain. Environ. Res., 22, 129, 2012.
• 7. GURUMOORTHY A.V.P., KHAN K.H. Polymers at interfaces: Biological and non-biological applications. Recent Res. Sci. Technol., 3, 80, 2011.
• 8. LIAN B., CHEN Y., ZHAO J., TENG H., ZHU L., YUAN S. Microbial flocculation by Bacillus mucilaginosus: applications and mechanisms. Bioresource Technol., 99, 4825, 2008.
• 9. MA F., ZHENG L.N., CHI Y. Applications of biological flocculants (BFs) for coagulation treatment in water purification: turbidity elimination. Chem. Biochem. Eng. Q., 22, 321, 2008.
• 10. VIJAYALAKSHMI S.P., RAICHUR A.M. The utility of Bacillus subtilis as a bioflocculant for fine coal. Colloid Surface B., 29, 265, 2003.
• 11. UGBENYEN A., COSA S., MABINYA L., BABALOLA O.O., AGHDASI F., OKOH A. Thermostable bacterial bioflocculant produced by Cobetia spp. isolated from Algoa Bay (South Africa). Int. J. Environ. Res. Public Health., 9, 2108, 2012.
• 12. GAOJ., BAO M., XIN Y., LIU Q., ZHANG Y. Characterization of a bioflocculant from a newly isolated Vagococcus sp. W31.J. Zhejiang University Sci. B., 7, 186, 2006.
• 13. COSA S., MABINYA L.V., OLANIRAN A.O., OKOH O.O., BERNARD K., DEYZEL S., OKOH A.I. Bioflocculant production by Virgibacillus sp. Rob isolated from the bottom sediments of Algoa Bay in the Eastern Cape, South Africa. Molecules. 16, 2431, 2011.
• 14. ZHANG J., LIU Z., WANG S., JIANG P. Characterization of a bioflocculant produced by the marine myxobacterium Nannocystis sp. NU2. Appl. Microbiol. Biot., 59, 517, 2002.
• 15. SHENG Y., ZHANG Q., SHENG Y., LI C., WANG H. Screening and flocculating properties of bioflocculant-producing microorganisms. Sci. Technol., 13, 289, 2006.
• 16. NAVARRETE-BOLANOS J.L., SERRATO-JOYA O., BOTELLO-ALVAREZ E., JIMENEZ-ISLAS H., CARDENA-MANRIQUEZ M., CONDE-BARAJAS E., RICOMARTINEZ R. Analyzing microbial consortia for biotechnological processes design. Trends Com. Curr. Res. Ed. Topics. Appl. Microbiol., 437, 2007.
• 17. WANG L., MA F., QU Y., SUN D., LI A., GUO J., YU B. Characterization of a compound bioflocculant produced by mixed culture of Rhizobium radiobacter F2 and Bacillus sphaeicus F6. World J. Microb. Biot., 27, 2559, 2011.
• 18. ZHANG Z., LIN B., XIA S., WANG X., YANG A.Production and application of a novel bioflocculant by multiple-microorganism consortia using brewery wastewater as carbon source. J. Environ. Sci., 19, 667, 2007.
• 19. KURANE R., HATAMOCHI K., KIYOHARA T., HIRAO M., TANIGUCHI Y. Production of a bioflocculant by Rhodococcus erythropolis S-1 grown on alcohols. Biosci. Biotech. Bioch., 58, 428, 1994.
• 20. CHANG W.C., SOON A.Y., IN H.O., SANG H.P. Characterization of an extracellular flocculating substance produced by a planktonic cyanobacterium, Anabaena sp. Biotechnol. Lett., 20, 643, 1998.
• 21. CHEN H., ZHANG J.F., JIANG P.J., YANG S.L., LIU Z.L. Composition and characterization of microbiological flocculant SC06. Environ. Chem., 21, 360, 2002.
• 22. MABINYA L.V., COSA S., MKHWETSHANA N., OKOH A.I. Halomonas sp. OKOH – a marine bacterium isolated from the bottom sediment of Algoa Bay – produces a polysaccharide bioflocculant: partial characterization and biochemical analysis of its properties. Molecules. 16, 4358, 2011.
• 23. DUBOIS M., GILLES K., HAMILTON J.K., REBERS P.A., SMITH F. Colorimetric method for determination ofsugars and related substances. Anal. Chem., 28, 350, 1956.
• 24. LOWRY O.H., ROSEBROUGH N.J., FARR A.L., RANDALL R.J. Protein measurement with the Folin Phenol reagent. J. Biol. Chem., 193, 265, 1951.
• 25. CESARETTI M., LUPPI E., MACCARI F., VOLPI N. A 96-well assay for uronic acid carbazole reaction. Carbohyd. Polym., 54, 59, 2003.
• 26. GONG W., WANG S., SUN X., LIU X., YUE Q., GAO B. Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment. Bioresource Technol., 99, 4668, 2008.
• 27. LEE S.H., SHIN W.S., SHIN M.C., CHOI S.J., PARK L.S. Improvement of water treatment performance byusing polyamine flocculants. Environ. Technol., 22, 653, 2001.
• 28. COSA S., MABINYA L.V., OLANIRAN A.O., OKOH A.I. Production and characterization of bioflocculant produced by Halobacillus sp. Mvuyo isolated from bottom sediment of Algoa Bay. Environ. Technol., iFirst, 1, 2012.
• 29. ZHENG Y., YE Z.L., FANG X.L., LI Y.H., CAI W.M. Production and characteristics of a bioflocculant produced by Bacillus sp. F19. Biol., 99, 7686, 2008.
• 30. PRASERTSAN P., DERMLIM W., DOELLE H., KENNEDY J.F. Screening, characterization and flocculating property of carbohydrate polymer from newly isolated Enterobacter cloacae WD7. Carbohyd. Polym., 66, 289, 2006.
• 31. XIONG Y., WANG Y., YU Y., LI Q., WANG H., CHEN R., HE N. Production and characterization of a novel bioflocculant from Bacillus licheniformis. Appl. Environ. Microbiol., 76, 2778, 2010.
• 32. KUMAR C.G., JOO H.S., KAVALI R., CHOI J.W., CHANG C.S. Characterization of an extracellular biopolymer flocculant from a haloakalophilic Bacillus isolate. J. Microbiol. Biotechn., 20, 837, 2004.
• 33. DENG S.B., BAI R.B., HU X.M., LUO Q. Characteristics of a bioflocculant produced by Bacillus mucilaginosus and its use in starch wastewater treatment. Appl. Microbiol. Biot., 60, 588, 2003.
• 34. YIM J.H.S., KIM J., AHN S.H., LEE H.K. Characterization of novel bioflocculant, p-KG03, from a marine dinoflagellate, Gyrodiumimpudicum KG03. Bioresource Technol., 98, 361, 2007.
• 35. LU W., ZHANG T., ZHANG D., LI C., WEN J., DU L. A novel bioflocculant produced by Enterobacter aerogenes and its use in defecating the trona suspension. Biochem. Eng. J., 27, 1, 2005.
• 36. HASSAN M.A.A., LI T.P., NOOR Z.Z. Coagulation and flocculation treatment of wastewater in textile industry using chitosan. J. Chem. Nat. Res. Engineer., 4, 43, 2009.
• 37. LIANG Z., HAN B., LIU H. Optimum conditions to treat high-concentration micro-plate slime water with bioflocculants. Mining Sci. Technol., 20, 0478, 2010.
• 38. BAJAJ I.B., SINGHAL R.S.Flocculation properties of poly (γ-Glutamic Acid) produced from Bacillus subtilis isolate. Food Bioprocess Technol. 4, 745, 2011.
• 39. YOKOI H., NATSUDA O., HIROSE J., HAYASHI S., TAKASAKI Y. Characteristics of a biopolymer flocculant produced by Bacillus sp. PY90. J. Ferment. Bioeng., 79, 378, 1995.