Characterization of bacteria of the genus Staphylococcus isolated from the eggs of Japanese quail (Coturnix coturnix japonica)

• Autorzy: Pyzik E. , Marek A.
• Języki publikacji: EN

Abstrakty

EN

The study attempted to analyse and characterize bacteria of the genus Staphylococcus isolated from the surface and contents of quail eggs, taking into account their phenotypic properties, biochemical reactions, antibiotic sensitivity patterns, and PCR to test for the presence of the mecA gene, which is responsible for resistance to methicillin. The study included 45 strains of the genus Staphylococcus isolated from the whites, yolks and shells of table quail eggs. The results obtained indicate that a fairly high percentage of the retail quail eggs tested were contaminated with Staphylococcus bacteria. Among the species isolated (11 in total), the most frequently occurring strains were of Staphylococcus hominis (26.7%), followed by Staphylococcus aureus (15.6%), S.xylosus and S. lentus (13.3% each), while percentages of the other Staphylococcus species were under 10%. The Staphylococcus strains tested had highly differentiated biochemical and enzymatic properties. As many as 7 biotypes were distinguished among the 7 S. aureus isolates, 6 biotypes within the species S. xylosus (6 strains tested), 5 biotypes among the 6 strains of S. lentus, but only 4 biotypes among the 12 S. hominis strains. The antibiotic sensitivity testing showed 15.5% of the strains to be resistant to one or more of the therapeutic agents tested. Moreover, some isolates exhibited intermediate sensitivity to the drugs, particularly to gentamicin (24.4%), neomycin (31.1%), streptomycin (46.7%) and Linco-Spectin (48.9%).

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2012
• Tom: 15
• Numer: 4
• Opis fizyczny: p.767-722,fig.,ref.

Twórcy

autor

Pyzik E.

• Department of Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland

autor

Marek A.

• Department of Avian Diseases, Institute of Biological Bases of Animal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland

Bibliografia

• Aarestrup FM, Agersø Y, Ahrens P, Jørgensen JC, Madsen M, Jensen LB (2000) Antimicrobial susceptibility and presence of resistance genes in staphylococci from poultry. Vet Microbiol 74: 353-364.
• Abulreesh HH, Organji SR (2011) The Prevalence of Multidrug- resistant Staphylococci in Food and the Environment of Makkah, Saudi Arabia. Res J Microbiol 6: 510-523.
• Bahrouz M, Al-Jaff A (2007) The risk of bacterial contamination in hen eggs of Sulaimani poultries. Journal of Zankoy Sulaimani 1: 63-71.
• Board RG, Tranter HS (1995) The microbiology of eggs. In: Standelman WJ, Cotterill OJ (eds) Egg Science and Technology. New York, Food Products Press – The Haworth Press Inc, pp 81-104.
• Brakstad OF, Maeland JA, Tveten Y (1993) Multiplex polymerase chain reaction for detection of genes for Staphylococcus aureus thermonuclease and methicillin resistance and correlation with oxacillin resistance. Acta Path Micro IM B 101: 681-688.
• Clinical and Laboratory Standards Institute (2011) Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement 31: 68-78.
• Gill VJ, Selepak ST, Williams EC (1983) Species identification and antibiotic susceptibilities of coagulase-negative staphylococci isolated from clinical specimens. J Clin Microbiol 18: 1314-1319.
• Grinsted J, Lacey RW (1973) Genetic variation of streptomycin resistance in clinical strains of Staphylococcus aureus. J Med Microbiol 6: 351-361.
• Khan SA, Nawaz MS, Khan A A, Cerniglia CE (2000) Transfer of erythromycin resistance from poultry to human clinical strains of Staphylococcus aureus. J Clin Microbiol 38: 1832-1838.
• Lyon BR, Gillespie MT, Byrne ME, May JW, Skurray RA (1987) Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon. J Med Microbiol 23: 101-110.
• Manie T, Khan S, Brozel VS, Veith WJ, Gouws PA (1998) Antimicrobial resistance of bacteria isolated from slaughtered and retail chickens in South Africa. Lett Appl Microbiol 26: 253-258.
• Miwa N, Kawamura A, Masuda T, Akiyama M (2001) An outbreak of food poisoning due to egg yolk reaction-negative Staphylococcus aureus. Int J Food Microbiol 64: 361-366.
• Młynarczyk G, Kochman M, Ławrynowicz M, Fordymacki E, Młynarczyk A, Jeljaszewicz J (1997) Wybrane właściwości szczepów Staphylococcus aureus fenotypowo wykazujących brak syntezy koagulazy. Medycyna Doświadczalna i Mikrobiologia 49: 5-12.
• Przybylska A (2001) Zatrucia I zakażenia pokarmowe w 1999 roku. Przegląd Epidemiologiczny 55: 93-101.
• Stępień-Pyśniak D, Marek A, Rzedzicki J (2009) Occurrence of bacteria of the genus Staphylococcus in table eggs descended from different sources. Pol J Vet Sci 12: 481-484.
• Thompson R, Khorazo D (1935) Susceptibility to lysozyme of Staphylococci. Proc Soc Exp Biol Med 33: 299-302.
• Wieliczko A, Król J, Piasecki T, Mazurkiewicz M, Staroniewicz Z (2002) Occurrence and characteristics of Staphylococci isolated from poultry. Med Weter 58: 348-352.

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