Docking for drug interface residues of modelled VPS33B of human with PtpA of Mycobacterium tuberculosis CDC1551

• Autorzy: Reddy K. M. , Pattathil M. D. , Jayachandra S. Y. , Parameshwar A. , Sulochana M. B.
• Języki publikacji: EN

Abstrakty

EN

VPS33B, a human Vacuolar Protein Sorting (VPS) protein which mediates the phagolysosomal fusion in macrophage of the eukaryotic organisms. This protein has a great role during the mycobacterial infections, which binds with the Mycobacterium protein tyrosine phosphatase A (PtpA). A single functional domain of PtpA has been identified using SMART domain databases, followed by finding the antigenicity of PtpA using CLC main workbench tool. The protein-protein interaction network predicts the interface of biological functions of proteins, built by using Cytoscape 2.8.3 version tool for manual literature survey of protein sets. According to the literature the specific interactivity of PtpA with VPS33B of human lead to pathogenesis, and provided a good platform to find the structure of VPS33B as it lacks the 3 dimensional structure in PDB. Homology Modelling of VPS33B provides a significant properties to design a specific drug through screening the drug databases (eDrug3D). The modelled protein has been validated through SAVES server maintained by NIH and UCLA with the standard Ramachandran plot with accuracy of 90.7 %. From our findings the interface residues are very crucial points which has been found through docking the modelled protein and Mycobacterium protein and interface residues were selected manually using PyMol software.

Słowa kluczowe

EN

antigenicity; protein-protein interaction; network; homology modelling; drug design; interface residue; Mycobacterium tuberculosis; man; protein; biological function

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2014
• Tom: 11
• Numer: 2
• Opis fizyczny: p.179-196,fig.,ref.

Twórcy

autor

Reddy K. M.

• Microbial Biodiversity and Bioinformatics Lab, Department of Biotechnology, Industrial Biotechnology, Gulbarga University, Gulbarga - 585106, India

autor

Pattathil M. D.

• Microbial Biodiversity and Bioinformatics Lab, Department of Biotechnology, Industrial Biotechnology, Gulbarga University, Gulbarga - 585106, India

autor

Jayachandra S. Y.

• Microbial Biodiversity and Bioinformatics Lab, Department of Biotechnology, Industrial Biotechnology, Gulbarga University, Gulbarga - 585106, India

autor

Parameshwar A.

• Microbial Biodiversity and Bioinformatics Lab, Department of Biotechnology, Industrial Biotechnology, Gulbarga University, Gulbarga - 585106, India

autor

Sulochana M. B.

• Microbial Biodiversity and Bioinformatics Lab, Department of Biotechnology, Industrial Biotechnology, Gulbarga University, Gulbarga - 585106, India

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