Przeżywalność Bacillus cereus w jelicie cienkim

• Autorzy: Berthold-Pluta A. , Pluta A. , Leszcz G.

Warianty tytułu

EN

Survival of Bacillus cereus in the small intestine

• Języki publikacji: PL

Abstrakty

EN

Bacillus cereus-mediated food poisoning of the diarrhoeal type is related to the production of the following extracellular factors by the bacteria: haemolysin BL (enterotoxin HBL), non-haemolytic toxin (NHE), and cytotoxin (CytK), as well as haemolysins IV and cereolysin O, both of which have been scarcely examined and discussed in the literature. This article reviews the literature containing current data that revise former theories on the pathogenesis of the diarrhoeal form of B. cereus-mediated food poisoning. The results of in vitro studies confirming the survival of vegetative cells in the stomach and intestine environment prove that the biological state of the examined bacteria does not have any influence on the occurrence of the diarrhoeal syndrome. After contaminated food has been ingested, B. cereus spores/vegetative cells pass the stomach and reach the small intestine. There the spores can germinate to become vegetative cells that multiply and produce enterotoxins. The enterotoxins, finally, affect the intestinal epithelium, which results in diarrhoea. So far this type of food poisoning has been attributed to enterotoxin activity, excluding any kind of interaction between the host and the microorganism. However, according to the results of the latest research, the interaction between the epithelial cells and the cells of B. cereus contributes to the occurrence of infection symptoms, and the adhesion of B. cereus to the intestinal epithelium is prerequisite for the onset of the diarrhoeal syndrome. This article illustrates different aspects of B.cereus survival inside the human gastrointestinal tract, paying special attention to its lower part, i. e. the small intestine. The effect of exposure to bile salts and other factors, as well as to the indigenous microflora of the gastrointestinal tract, on B.cereus survival has been discussed. The article also elucidates issues relating to the mechanism of bacterial-epithelial cell cross-talk (interaction), which is induced when a pathogen comes into contact with enterocytes.

Słowa kluczowe

PL

zatrucia pokarmowe; zywnosc; zanieczyszczenia mikrobiologiczne; patogeneza; Bacillus cereus; przewod pokarmowy; jelito cienkie; przezywalnosc; mikroflora jelitowa; sole zolciowe

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2011
• Tom: 67
• Numer: 11
• Opis fizyczny: s.733-736,bibliogr.

Twórcy

autor

Berthold-Pluta A.

• Zakład Biotechnologii Mleka, Katedra Biotechnologii, Mikrobiologii i Oceny Żywności, Wydział Nauk o Żywności, Szkoła Główna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159c, 02-787 Warszawa

autor

Pluta A.

autor

Leszcz G.

Bibliografia

• 1. Andersson A., Granum P. E., Rönner U.: The adhesion of Bacillus cereus spores to epithelial cells might be an additional virulence mechanism. Int. J. Food Microbiol. 1998, 39, 93-99.
• 2. Begley M., Gahan C. G. M., Hill C.: Bile stress response in Listeria monocytogenes LO28: adaptation, cross-protection and identification of genetic loci involved in bile resistance. Appl. Environ. Microbiol. 2002, 68, 6005-6012.
• 3. Begley M., Gahan C. G. M., Hill C.: The interaction between bacteria and bile. FEMS Microbiol. Rev. 2005, 29, 625-651.
• 4. Clavel T., Carlin F., Dargaignaratz C., Lairon D., Nguyen-The C., Schmitt P.: Effects of porcine bile on survival of Bacillus cereus vegetative cells and Haemolysin BL enterotoxin production in reconstituted human small intestine media. J. Appl. Microbiol. 2007, 103, 1568-1575.
• 5. Faille C., Jullien C., Fontaine F., Bello-Fontaine M., Slomianny C., Benezech T.: Adhesion of Bacillus cereus spores and Escherichia coli cells to inert surfaces: role of surface hydrophobicity. Can. J. Microbiol. 2002, 48, 728-738.
• 6. Flahaut S., Giard J. C., Benachour A., Boutibonnes P., Auffray A.: Defense against lethal treatments and de novo protein synthesis induced by NaCl in Enterococcus faecalis ATCC 19433. Arch. Microbiol. 1996, 165, 317-324.
• 7. Fujisawa T., Mori M.: Influence of bile salts on β-glucuronidase activity of intestinal bacteria. Lett. Appl. Microbiol. 1996, 22, 271-274.
• 8. Gänzle M. G., Hertel C., van der Vossen J. M. B. M., Hammes W. P.: Effect of bacteriocin-producing lactobacilli on the survival of Escherichia coli and Listeria in a dynamic model of the stomach and the small intestine. Int. J. Food Microbiol. 1999, 48, 21-35.
• 9. Gomez-Zavaglia A., Kociubinski G., Perez P., Disalvo E., De Antonio G.: Effect of bile on the lipid composition and surface properties of bifidobacteria. J. Appl. Microbiol. 2002, 93, 794-799.
• 10. Gominet M., Slamti L., Gilois N., Rose M., Lereclus D.: Oligopeptide permease is required for the expression of the Bacillus thuringiensis plcR regulon and for virulence. Mol. Microbiol. 2001, 40, 963-975.
• 11. Jaradat Z. W., Bhunia A. K.: Adhesion, invasion and translocation characteristics of Listeria monocytogenes serotypes in Caco-2 cell and mouse models. Appl. Environ. Microbiol. 2003, 69, 3640-3645.
• 12. Kristoffersen S. M., Ravnum S., Tourasse N. J., Økstad O. A., Kolstø A.-B., Davies W.: Low concentrations of bile salts induce stress responses and reduce motility in Bacillus cereus ATCC 14570. J. Bacteriol. 2007, 189, 5302-5313.
• 13. Leverrier P., Dimova D., Pichereau V., Auffray Y., Boyaval P., Jan G.: Susceptibility and adaptive response to bile salts in Propionibacterium freudenreichii: physiological and proteomic analysis. Appl. Environ. Microbiol. 2003, 69, 3809-3818.
• 14. Libudzisz Z.: Mikroflora przewodu pokarmowego człowieka i jej wpływ na organizm, [w:] Gawęcki J., Libudzisz Z. (red.): Mikroorganizmy w żywności i żywieniu. Wydawnictwo Akademii Rolniczej, Poznań 2006, 31-41.
• 15. Minnaard J., Delfederico L., Vasseur V., Hollmann A., Rolny I., Semorile L., Perez P. F.: Virulence of Bacillus cereus: A multivariate analysis. Int. J. Food Microbiol. 2007, 116, 197-206.
• 16. Minnaard J., Humen M., Perez P. F.: Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells. J. Food Prot. 2001, 64, 1535-1541.
• 17. Minnaard J., Lievin-Le Moal V., Coconnier M. H., Servin A. L., Perez P. F.: Disassembly of F-actin cytoskeleton after interaction of Bacillus cereus with fully differentiated human intestinal Caco-2 cells. Inf. Immun. 2004, 72, 3106-3112.
• 18. Neumann M., Goderska K., Grajek K., Grajek W.: Modele przewodu pokarmowego in vitro do badań nad biodostępnością składników odżywczych. Żywność. Nauka. Technologia. Jakość 2006, 46, 30-45.
• 19. Peng I. S., Tsai W. C., Chou C. C.: Surface characteristics of Bacillus cereus and its adhesion to stainless steel. Int. J. Food Microbiol. 2001, 65, 105-111.
• 20. Powell A. A., LaRue J. M., Martinez J. D.: Bile acid hydrophobicity is correlated with induction of apoptosis and/or growth arrest in HTC116 cells. Bioch. J. 2001, 356, 481-486.
• 21. Rajagopalan N., Lindenbaum S.: The binding of Ca²⁺ to taurine and glycineconjugated bile salts micelles. Biochem. Biophys. 1982, 711, 66-74.
• 22. Ramarao N., Lereclus D.: Adhesion and cytotoxicity of Bacillus cereus and Bacillus thuringiensis to epithelial cells are FlhA and PlcR dependent, respectively. Microbes Infection 2006, 8, 1483-1491.
• 23. Rönner U., Husmark U., Henriksson A.: Adhesion of Bacillus spores in relation to hydrophobicity. J. Appl. Bacteriol. 1990, 75, 550-556.
• 24. Røssland E., Andersen Borge G. I., Langsrud T., Sørhaug T.: Inhibition of Bacillus cereus by strains of Lactobacillus and Lactococcus in milk. Int. J. Food Microbiol. 2003, 89, 205-212.
• 25. Røssland E., Langsrud T., Granum P. E., Sørhaug T.: Production of antimicrobial metabolites by strains of Lactobacillus or Lactococcus co-cultured with Bacillus cereus in milk. Int. J. Food Microbiol. 2005, 98, 193-200.
• 26. Sanyal A., Shiffman M. L., Hirsch J. I., Moore E. W.: Premicellar taurocholate enhances ferrous iron uptake from all regions of rat small intestine. Gastroenterology 1991, 101, 382-388.
• 27. Shimakawa Y., Matsubara S., Yuki N., Ikeda M., Ishikawa F.: Evaluation of Bifidobacterium breve Yakult-fermented soymilk as a probiotic food. Int. J. Food Microbiol. 2003, 81, 131-136.
• 28. Sutherland A. D., Limond A. M.: Influence of pH and sugars on the growth and production of diarrhoeagenic toxin by Bacillus cereus. J. Dairy Res. 1993, 60, 2330-2335.
• 29. Velkinburgh J. C. van, Gunn J. S.: PhoP-PhoQ-regulated loci are required for enhanced bile resistance in Salmonella ssp. Inf. Immun. 1999, 67, 1614-1622.
• 30. Wijnands L. M., Dufrenne J. B., van Leusden F. M., Abee T.: Germination of Bacillus cereus spores is induced by germinants from differentiated Caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine. Appl. Environ. Microbiol. 2007, 73, 5052-5054.
• 31. Wijnands L. M., Dufrenne J. B., van Leusden F. M.: Bacillus cereus: characteristics, behavior in the gastro-intestinal tract and interaction with Caco-2 cells. The National Institute for Public Health and the Environment, Bilthoven 2005, Report RIVM 250912003/2005.
• 32. Wijnands L. M., Dufrenne J. B., Zwietering M. H., van Leusden F. M.: Spores from mesophilic Bacillus cereus strains germinate better and grow faster in simulated gastro-intestinal conditions than spores from psychrotrophic strains. Int. J. Food Microbiol. 2006, 112, 120-128.
• 33. Zarate G., Gonzalez S., Chaia A. P., Olivier G.: Effect of bile on the β-galactosidase activity of dairy propionibacteria. Lait 2000, 80, 267-276.