INTRODUCTION: Cerebrovascular diseases are the principal causes of mortality and disability worldwide. Creation of a repeatable, very precise, and minimally invasive animal model of ischemic stroke is necessary. A well‑designed model will facilitate future research on new possibilities of treatment of ischemic stroke. The penumbra is the area surrounding necrosis in ischemic stroke. It is a strategic area for therapy. Survival and restoration of correct circulation is the goal of treatment of ischemic stroke. Protein HuR is a member of the ELAVL protein family. This protein has one of the best-known functions in stabilizing mRNAs in order to regulate gene expression. AIM(S): The first aim of ourresearch wasto develop, test, and select the most favorable parameters in a model of photothrombotic ischemic stroke in rat. The second goal was to determine the location of the HuR protein during an ischemic stroke, especially in the penumbra. METHOD(S): Phototrombotic ischemia was produced in 54 male Long‑Evans rats by evoking the clotting of blood inside the cerebral vessels of the rat, through activation of Bengalirose with the light of 3200 K energy. Bengalrose was given by a tail vein. We tested different exposure times for light (10 min/ 15 min/ 20 min) and different life times ofrats from surgery to euthanasia (6 h, 12 h, 24 h, 48 h, 7 days, and 14 days). RESULTS: We performed immunohistochemistry (IHC) tests on brain tissue after ischemic stroke. After analyzing the results,we obtained the best parameters: 15 minexposure to light and life time (12 h – 48 h). These parameters made it possible to obtain a small necrosis area and a large penumbra area. We observed increased concentration of protein HuR in the cytoplasm of panumbra neurons between 12 h – 48 h afterstroke. CONCLUSIONS: Our model of small cortical ischemic stroke is repeatable, very precisely located, and low invasive. During ischemic stroke, there is increased transport of HuR proteins to the cytoplasm of neurons, especially in the penumbra as compared to healthy tissue.