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2002 | 07 | 2A |
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Application of GISH and AFLP techniques for identification of Lolium-Festuca introgressions

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At present, breeding programmes aimed at combining advantageous traits within the Lolium-Festuca complex, are mainly focused on introgression procedures. One principal objective, is the transfer of genes conferring resistance to abiotic stresses from Festuca species (F. pratensis, F. arundinacea and F. glaucescens) into Lolium multiflorum and L. perenne germplasm. In our experiments, two different hybrids: triploid - L. multiflorum (4x) x F. pratensis (2x) and pentaploid - F. arundinacea (6x) x L. multiflorum (4x) were backcrossed twice onto L. multiflorum cultivars, and numerous BC2 progeny generated. BC2 plants from both combinations were tested in field and/or simulated conditions for winter hardiness and drought resistance. GISH (genomic in situ hybridisation) analyses were then performed on the most winter hardy and drought resistant plants to locate putative genes for stress resistance. Using resistant L. multiflorum genotypes with a single Festuca chromatin segment, it was possible to allocate AFLP (amplified fragment length polymorphism) markers specific to that segment. Markers associated with genes conferring stress resistance facilitate marker-assisted selection programmes to obtain new, more persistent grass cultivars. Preliminary results of GISH analysis, to identify Festuca chromosome segments in L. multiflorum introgression lines and to find segment-specific AFLP markers, are presented.
Opis fizyczny
  • Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland
  • Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland
  • Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, SY23 3EB, U.K.
  • Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland
  • 1. Thomas, H. M. and Humphreys, M. O. Progress and potential of interspecific hybrids of Lolium and Festuca. J. Agric. Sci, 117 (1991) 1-8.
  • 2. Jauhar, P. P. Chromosome relationships between Lolium and Festuca (Gramineae). Chromosoma 52 (1975) 103-121.
  • 3. Zwierzykowski, Z. Interspecific and intergeneric hybrids of the Lolium- Festuca complex obtained in Poland in the years 1964-1994 and maintained in the collection at the Institute of Plant Genetics in Poznań. J. Appl. Genet. 37 (1996) 79-100.
  • 4. Thomas, H. M., Morgan, W. G., Meredith, M. R., Humphreys, M. W., Thomas, H. and Leggett, J. M. Identification of parental and recombined chromosomes in hybrid derivatives of Lolium multiflorum x Festuca pratensis by genomic in situ hybridisation. Theor. Appl. Genet. 88 (1994) 909-913.
  • 5. King, I. P., Morgan, W. G., Armstead, I. P., Harper, J. A., Hayward, M. D., Bollard, A., Nash, J. V., Forster, J. W. and Thomas, H. M. Introgression mapping in the grasses. I. Introgression of Festuca pratensis chromosomes and chromosome segments into Lolium perenne. Heredity 81 (1998) 462- 467.
  • 6. Zwierzykowski, Z., Tayyar, R., Brunell, M. and Lukaszewski, A. J. Genome recombination in intergeneric hybrids between tetraploid Festuca pratensis and Lolium multiflorum. J. Heredity 89 (1998) 324-328.
  • 7. Zwierzykowski, Z., Lukaszewski, A. J., Naganowska, B. and Leśniewska, A. The pattern of homoeologous recombination in triploid hybrids of Lolium multiflorum with Festuca pratensis. Genome 42 (1999) 720-726.
  • 8. Armstead, I. P., Bollard, A., Morgan, W. G., Harper, J. A., King, I. P., Jones, R. N., Forster, J. W., Hayward, M. D. and Thomas, H. M. Genetic and physical analysis of a single Festuca pratensis chromosome segment substitution in Lolium perenne. Chromosoma 110 (2001) 52-57.
  • 9. Humphreys, M. W. and Pasakinskiene, I. Chromosome painting to locate genes for drought resistance transferred from Festuca arundinacea to Lolium multiflorum. Heredity 77 (1996) 530-534.
  • 10. Humphreys, M. W., Thomas, H. M., Harper, J. A., Morgan, M. G., James, A. R., Zare, A. G. and Thomas, H. Dissecting drought- and cold-tolerance traits in the Lolium-Festuca complex by introgression mapping. New Phytol. 137 (1997) 55-60.
  • 11. Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. AFLP: a new technique for DNA fingerprinting. Nuci. Acids Res. 23 (1995) 4407-4414.
  • 12. Murray, M. G. and Thompson, W. F. Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 8 (1980) 4321-4325.
  • 13. Schwarzacher, T., Leitch, A. R. and Heslop-Harrison, J. S. DNA:DNA in situ hybridisation-methods for light microscopy. In: Plant Cell Biology: A Practical Approach, (Harris, N. and Oparka, K., Eds), Oxford University Press, Oxford, 1994, 127-155.
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