Calcium (Ca2+) expression and intensity in cumulus-oocyte complex (COCs) in Kacang goat after vitrification

• Autorzy: Widjiati W. , Faizah Z. , Darsini N. , Hendrawan V.F. , Karima H.N. , Chotimah C. , Sumitro S.B. , Yustinasari L.R. , Kasman A.A.M.N. , Ntoruru J.M. , Luqman E.M.
• Języki publikacji: EN

Abstrakty

EN

The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2022
• Tom: 25
• Numer: 1
• Opis fizyczny: p.19-26,fig.,ref.

Twórcy

autor

Widjiati W.

• Post Graduate School of Universitas Airlangga Surabaya, Indonesia

autor

Faizah Z.

• Department of Biomedical Science, Faculty of Medicine Universitas Airlangga Surabaya, Indonesia

autor

Darsini N.

• Department of Biomedical Science, Faculty of Medicine Universitas Airlangga Surabaya, Indonesia

autor

Hendrawan V.F.

• Department of Reproduction, Faculty of Veterinary Medicine, Universitas Brawijaya Malang, Indonesia

autor

Karima H.N.

• Bio-Science Central Laboratory, Universitas Brawijaya Malang, Indonesia

autor

Chotimah C.

• Bio-Science Central Laboratory, Universitas Brawijaya Malang, Indonesia

autor

Sumitro S.B.

• Department of Biology, Faculty of Science, Universitas Brawijaya Malang, Indonesia

autor

Yustinasari L.R.

• Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga Surabaya, Indonesia

autor

Kasman A.A.M.N.

• Faculty of Health Science, Universitas Muhammadiyah Mataram, Mataram

autor

Ntoruru J.M.

• Research Assistant, Meru University, Kenya

autor

Luqman E.M.

• Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga Surabaya, Indonesia

Identyfikatory

DOI

10.24425/pjvs.2022.140836