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2013 | 69 | 01 |

Tytuł artykułu

Badania morfologiczne oligodendrocytów istoty szarej środkowej u starych szczurów

Warianty tytułu

EN
Morphological central gray matter oligodendrocytes in old rats

Języki publikacji

PL

Abstrakty

EN
Currently the structure and function of oligodendrocytes in the central nervous system (cns) in young individuals is known; however, there is no information about their morphology in the central gray matter (substantia grisea centralis-SGC) in old animals. The aim of this study was to investigate the morphology of oligodendrocytes in the semithin SGC sections of 6-month (5 pcs) and 3-year-old (5 pcs) male Wistar rats. The animals were anaesthetized with ketamine, perfused with fixative through the left ventricle, and the midbrains containing SGC were taken. Semithin sections were analyzed morphologically and photographs were made in light microscope Axiolab (Zeiss). In the semithin sections of the SGC of 6-month-old adult rats oligodendrocytes with mostly light and medium density cytoplasm were observed. Oligodendrocytes were located by different structures: blood vessels, neurons and astrocytes. Sometimes two cells with dark cytoplasm were side by side or near oligodendrocytes with a medium cytoplasm density. In old male rats oligodendrocytes with dark cytoplasm located at the capillary blood vessels, neurons and astrocytes were dominant in the SGC. Cells with light cytoplasm, which occurred mainly in blood vessels, were rarely encountered. The results indicate that in the SGC of 3-years-old individuals older forms with a dark cytoplasm are dominant, in contrast to 6-month-old rats which have younger oligodendrocytes with light cytoplasm.

Wydawca

-

Rocznik

Tom

69

Numer

01

Opis fizyczny

s.47-51,fot.,bibliogr.

Twórcy

  • Zakład Histologii i Embriologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin
  • Zakład Histologii i Embriologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin

Bibliografia

  • 1.Andres M. A., Calle E., Lafarga M.: Age-induced hypertrophy of astrocytes in rat supraoptic nucleus: a cytological, morphometric, and immunocytochemical study. Anat. Rec. 1995, 243, 129-144.
  • 2.Bachevalier J., Landis L. S., Walker L. C., Brickso M., Mishkin M., Price D. L.: Aged monkeys exhibit behavioral deficits indicative of widespread cerebral dysfunction. Neurobiol. Aging 1991, 12, 99-111.
  • 3.Bencovic S. A., Connor J. R.: Ferritin, transferin and iron in selected regions of the adult and aged rat brain. J. Comp. Neurol. 1993, 338, 97-113.
  • 4.Borras D., Ferres I., Pumarola M.: Age-related changes in the brain of the dog. Vet. Pathol. 1999, 36, 202-211.
  • 5.Cavallotti D., Cavallotti C., Pescosolido N., Iannetti G. D., Pacella E.: A morphometric study of age changes in the rat optic nerve. Ophthalmologica 2001, 215, 366-371.
  • 6.Cerghet M., Skiff R. P., Beisert D., Hang Z., Mullis C., Ghandour M. S.: Proliferation and heath of oligodendrocytes and myelin proteins are differentially regulated in male and female rodents. J. Neurosci. 2006, 26, 1439-1447.
  • 7.Diamond M. C., Johnson R. E., Gold M. W.: Changes in neuron number and size and glial number in the young, adult and aging rat medial occipital cortex. Behav. Biol. 1977, 20, 409-418.
  • 8.Ling A. E., Leblond C. P.: Investigation of glial cells in semithin sections. II. Variation with age in the numbers of the various glial cell types in rat cortex and corpus callosum. J. Comp. Neurol. 1973, 149, 73-81.
  • 9.Ling A. E., Paterson J. A., Privat A., Mori S., Leblond C. P.: Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain of young rats J. Comp. Neurol. 1973, 149, 43-71.
  • 10.Lintl P., Braak H.: Loss of intracortical myelinated fibers: a distinctive age-related alteration in the human striate area. Acta Neuropathol. 1983, 61, 178-182.
  • 11.Mori S.: Light and electron microscopic features and frequencies of the glial cell present in the cerebral cortex of the rat brain. Arch. Histol. Jnp 1972, 34, 231-244.
  • 12.Mori S. I., Leblond C. P.: Electron microscopic identification of three classes of oligodendrocytes and a preliminary study of their proliferative activity in the corpus callosum of young rats. J. Comp. Neurol. 1970, 139, 1-30.
  • 13.Muton L. J., Klop E.-M., Broman J., Zhang M., Holstege G.: Lateral cervical nucleus projections to periaqueductal gray matter of cat. J. Comp. Neurol. 2004, 471, 434-445.
  • 14.Pakkenberg B., Pelvig D., Marner L., Bundgaard M. J., Gundersen H. J. G., Nyengaard J. R., Regeur L.: Aging and the human neocortex. Exp. Gerontol. 2003, 38, 95-99.
  • 15.Peinado M. A., Quesada A., Pedrosa J., Torres M. I., Martinem M., Esteban F. J., Del Moral M., Hernandez R., Rodrigo J.: Quantitative and ultrastructural changes in glia and pericytes in the parietal cortex of the aging rat. Microscopy Res. Techn. 1998, 43, 34-42.
  • 16.Peters A.: The effect of normal aging on myelinated nerve fibres in monkey central nervous system. Frontier Neuroanat. 2009, 3, 1-10.
  • 17.Peters A., Sethares C.: Is there remyelination during aging of the primate central nervous system? J. Comp. Neurol. 2003, 460, 238-254.
  • 18.Peters A., Sethares C.: Oligodendrocytes, their progenitors and other neuroglial cells in the aging primate cerebral cortex. Cerebrebral Cortex 2004, 14, 995-1007.
  • 19.Rees S.: A quantitative electron microscope study of the ageing human cerebral cortex. Acta Neuropathol. 1976, 36, 347-362.
  • 20.Sandell J. H., Peters A.: Disrupted myelin and axon loss in the anterior commissure of the aged rhesus monkey. J. Comp. Neurol. 2003, 466, 14-30.
  • 21.Sandell J. H., Peters A.: Effects of age on the glial cells in the rhesus monkey optic nerve. J. Comp. Neurol. 2002, 445, 13-28.
  • 22.Sato T.: A modified method for lead staining of thin sections. Electron Microsc. 1968, 17, 158-159.
  • 23.Somjen G. G.: Nervenkitt: Notes on the History of the Concept of Neuroglia. Glia 1988, 1, 2-9.
  • 24.Sturrock R. R.: Identification of mitotic oligodendrocytes in semithin sections of the developing mouse corpus callosum and hippocampal commissure. J. Anat. 1983, 137, 47-55.
  • 25.Vaughan D. W., Peters A.: Neuroglial cells in the cerebral cortex of rats from young aldulthood to old age: an electron microscope study. J. Neurocytol. 1974, 3, 404-433.
  • 26.Vianna D. M. I., Brandão M. I.: Anatomical connections of the periaqueductal gray: specific neural substratem for different kinds of fear. Braz. J. Med. Biol. Res. 2003, 36, 557-566.
  • 27.Watanabe M., Toyama Y., Nishiyama A.: Differentiation of proliferating NG2-positive glial progenitor cells in a remyelinating lesion. J. Neurosci. Res. 2002, 69, 826-836.
  • 28.Wawrzyniak-Gacek A.: Distribution o various types of oligodendrocytes and cellular localisation of iron in the frontal cortex of the adult rat. Folia Morphol. 2002, 61, 115-121.
  • 29.Xiaoqin Zhu, Bergles D. E., Nishiyama A.: NG2 cells generate both oligodendrocytes and gray matter astrocytes. Development 2008, 135, 145-157.

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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