Culture-dependent analysis of 16S rRNA sequences associated with the rhizosphere of Lemna minor and assessment of bacterial phenol-resistance: plant/bacteria system for potential bioremediation - part II

• Autorzy: Radulovic O. , Petric M. , Raspor M. , Stanojevic O. , Janakiev T. , Tadic V. , Stankovic S.
• Języki publikacji: EN

Abstrakty

EN

In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2019
• Tom: 28
• Numer: 2
• Opis fizyczny: p.811-822,fig.,ref.

Twórcy

autor

Radulovic O.

• Institute for Chemistry, Technology and Metallurgy, University of Belgrade, Njegoseva 12, Belgrade, Serbia

autor

Petric M.

• Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Despota Stefana 142 Street, Belgrade, Serbia

autor

Raspor M.

• Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Despota Stefana 142 Street, Belgrade, Serbia

autor

Stanojevic O.

• Faculty of Biology, University of Belgrade, Studentski Trg 16, Belgrade, Serbia

autor

Janakiev T.

• Faculty of Biology, University of Belgrade, Studentski Trg 16, Belgrade, Serbia

autor

Tadic V.

• Mining and Metallurgy Institute, Zeleni Bulevar 35, 19219 Bor, Serbia

autor

Stankovic S.

• Faculty of Biology, University of Belgrade, Studentski Trg 16, Belgrade, Serbia

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Identyfikatory

DOI

10.15244/pjoes/81687