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2014 | 13 | 2 |
Tytuł artykułu

Morpho-histological aspects of adventitious shoot formation without plant growth regulators in seed explants of Capsicum annuum L., and impact of preculture on regeneration

Autorzy
Warianty tytułu
PL
Morfologiczno-histologiczne aspekty formowania pędów przybyszowych bez regulatorów wzrostu w eksplantatach nasion Capsicum annuum L. oraz wpływ prekultury na regenerację
Języki publikacji
EN
Abstrakty
EN
The physiological state of plant material is the crucial endogenous factor at the explant choice for plant regeneration. The phases of germination characterised by various, following each other biochemical and developmental processes can affect the organogenesis capability. This research examined the morphological and anatomical events during the early stages of organogenesis and plant regeneration in explants derived from seeds of Capsicum annuum L., cv. Bryza preincubated under high humidity conditions from 0 to 6 days and next cultured on MS medium without PGRs. The early stage of de novo shoot formation reminded leaf differentiation in planta. First the leaves began to differentiate as spherical and tongue shaped structures from epidermis and subjacent layers of the explants about the 7th day of culture. In some cases nearly at the base of previously formed leaf and even on its petiole one or two leaves as well as shoot apex in their axils were induced thereby forming young shoot which underwent elongation and whole plant regeneration after 2 subculturing. More advanced developmentally structures of adventitious shoot were obtained while prolonging preculture duration. This was the favourable effect on the shoot differentiation, their elongation and plant regeneration as seed submitted preculture for 3, 4, 5 days however, seeds not treated with preculture revealed the best response as regard to shoot primordium formation at the earliest stage.
PL
Stan fizjologiczny materiału roślinnego jest kluczowym endogennym czynnikiem przy wyborze eksplantatu do regeneracji roślin. Fazy kiełkowania, charakteryzujące się różnymi następującymi po sobie biochemicznymi i rozwojowymi procesami, mogą mieć wpływ na zdolności do organogenezy. W pracy tej przebadano morfologiczne i anatomiczne przemiany podczas wczesnych etapów organogenezy i regeneracji roślin w eksplantatach pochodzących z nasion Capsicum annuum L. odmiany Bryza, które wstępnie inkubowano w wilgotnych warunkach od 0 do 6 dni, a nastpnie utrzymywano w kulturach na pożywce MS bez regulatorów wzrostu (R.W.). Wczesne stadia formowania pędów de novo przypominały różnicowanie liścia in planta. Pierwsze liście zaczęły różnicować się jako struktury o kształcie sferycznym i języczkowatym w epidermie i warstwach leżących tuż pod nią około 7 dnia kultury eksplantatów, w niektórych przypadkach bardzo blisko podstawy wcześniej powstałego liścia, a nawet na jego ogonku. Jeden a nawet dwa liście i wierzchołek pędu w ich kątach były indukowane, formując w ten sposób młody pęd, który ulegał elongacji i pełnej regeneracji do rośliny po 2 pasażach. Wydłużając czas prekultury, otrzymywano struktury bardziej zaawansowane rozwojowo. Korzystny wpływ na różnicowanie pędów, ich elongację i regenerację roślin zaznaczyć się, kiedy nasiona poddano prekulturze przez 3, 4 i 5 dni, chociaż nie traktowane ujawniły najlepszą odpowiedź odnośnie formowania zawiązków pędowych w ich najwcześniejszym etapie.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
13
Numer
2
Opis fizyczny
p.135-150,fig.,ref.
Twórcy
autor
  • Department of Plant Physiology and Fundamentals of Biotechnology, University of Technology and Life Sciences in Bydgoszcz, Bernardynska 6, 85-029 Bydgoszcz, Poland
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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