Prevalence and molecular characterisation of Schistosoma haematobium among primary school children in Kebbi State, Nigeria

• Autorzy: Umar S. , Shinfafi S.H. , Hudu S.A. , Neela V. , Suresh K. , Nordin S.A. , Malina O.
• Języki publikacji: EN

Abstrakty

EN

Schistosomiasis is the major source of morbidity in Sub-Saharan Africa and Asia. It is estimated that 207 million people are infected, of which 97% are in Africa. The aim of this study was the determining of prevalence as well as the phylogeny of S. haematobium among school children in Argungu Emirate, Kebbi State Nigeria. A total of 325 urine samples was collected from school children between 7 to 14 years. S. heamatobium eggs was examined under dissecting microscope and DNA was extracted from urine sample and COX1 gene was amplified by nested PCR. The PCR products were purified, sequenced and analysed. This study showed a prevalence of 32.09%, with male pupils having the highest prevalence. S. haematobium infections in children who fetch water in the river have 24 times higher risk of being infected while those who bath in the river have 158 times higher risk of being infected. Our sequences were phylogenetically related to S. haematobium isolate U82266 from Kenya and consistence with the predominant species in Africa. This was the first S. haematobium and S. mansoni co-infection reported in Nigeria. S. haematobium infection is prevalent among school age and significantly associated with water contact.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2017
• Tom: 63
• Numer: 2
• Opis fizyczny: p.133-139,fig.,ref.

Twórcy

autor

Umar S.

• Department of Microbiology and Parasitology, Usmanu Danfodiyo University Teaching Hospital Sokoto, 80002 Sokoto State, Nigeria

autor

Shinfafi S.H.

• Department of Microbiology and Parasitology, Usmanu Danfodiyo University Teaching Hospital Sokoto, 80002 Sokoto State, Nigeria

autor

Hudu S.A.

• Department of Medical Microbiology and Parasitology, Faculty of Basic Medical Sciences, College of Health Sciences, Usmanu Danfodiyo University Sokoto, 8400001 Sokoto State, Nigeria

autor

Neela V.

• Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 43400, UPM Serdang, Selangor, Malaysia

autor

Suresh K.

• Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 43400, UPM Serdang, Selangor, Malaysia

autor

Nordin S.A.

• Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 43400, UPM Serdang, Selangor, Malaysia

autor

Malina O.

• Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia 43400, UPM Serdang, Selangor, Malaysia

Bibliografia

• [1] Steinmann P., Keiser J., Bos R., Tanner M., Utzinger J. 2006. Schistosomiasis and water resources development: systematic review, meta-analysis, and estimates of people at risk. The Lancet Infectious Diseases 6: 411-425. http://dx.doi.org/10.1016/S1473-3099(06)70521-7
• [2] Clements A.C.A., Firth S., Dembelé R., Garba A., Touré S., Sacko M., Landouré A., Bosqué-Oliva E., Barnett A.G., Brooker S., Fenwick A. 2009. Use of Bayesian geostatistical prediction to estimate local variations in Schistosoma haematobium infection in western Africa. Bulletin of the World Health Organization 87: 921-929. doi:10.2471/blt.08.058933
• [3] Gray D.J., Ross A.G., Li Y.S., McManus D.P. 2011. Diagnosis and management of schistosomiasis. British Medical Journal 342: d2651. https://doi.org/10.1136/bmj.d2651
• [4] Shuaibu H.A., Jimoh A.O. 2011. Assessment of socioeconomic, demographic and health problems of Al-Majiri in Sokoto State, North-Western Nigeria. International Journal of Tropical Medicine 6: 58-60. doi:10.3923/ijtmed.2011.58.60
• [5] Ukoroije B.R., Abowei J. 2012. Some occupational diseases in culture fisheries management and practices. Part two: Schistosomiasis and Filariasis. International Journal of Fisheries and Aquatic Studies 1: 64-71.
• [6] Nithiuthai S., Anantaphruti M.T., Waikagul J., Gajadhar A. 2004. Waterborne zoonotic helminthiases. Veterinary Parasitology 126:167-193. http://doi.org/10.1016/j.vetpar.2004.09.018
• [7] Akinwale O., Ajayi M., Akande D., Adeleke M., Gyang P., Adeneye A., Dike A. 2009. Prevalence of Schistosoma haematobium infection in a neglected community, south western Nigeria. International Journal of Health Research 2: 149-155. http://dx.doi.org/10.4314/ijhr.v2i2.55408
• [8] Bello A., Jimoh A.O., Shittu S.B., Hudu S.A. 2014. Prevalence of urinary schistosomiasis and associated haemato-proteinuria in Wurno Rural Area of Sokoto State, Nigeria. Orient Journal of Medicine 26:114-121.
• [9] Rollinson D., Knopp S., Levitz S., Stothard J.R., Tchuem Tchuenté L-A., Garba A., Mohammed K.A., Schur N., Person B., Colley D.G., Utzinger J. 2013. Time to set the agenda for schistosomiasis elimination. Acta Tropica 128: 423-440. http://doi.org/10.1016/j.actatropica.2012.04.013
• [10] Ballard J.W.O., Whitlock M.C. 2004. The incomplete natural history of mitochondria. Molecular Ecology 13: 729-744. doi:10.1046/j.1365-294X.2003.02063.x
• [11] Ibironke O.A., Phillips A.E., Garba A., Lamine S.M, Shiff C. 2011. Diagnosis of Schistosoma haematobium by detection of specific DNA fragments from filtered urine samples. The American Journal of Tropical Medicine and Hygiene 84: 998-1001. https://doi.org/10.4269/ajtmh.2011.10-0691
• [12] Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725-2729. https://doi.org/10.1093/molbev/mst197
• [13] Agatsuma T., Iwagami M., Liu C.X., Saitoh Y., Kawanaka M., Upatham S., Qui D., Higuchi T. 2001. Molecular phylogenetic position of Schistosoma sinensium in the genus Schistosoma. Journal of Helminthology 75: 215-221. https://doi.org/10.1079/joh200156
• [14] Biu A.A., Kolo H.B., Agbadu E.T. 2009. Prevalence of Schistosoma haematobium infection in school aged children of Konduga Local Government Area, Northeastern Nigeria. International Journal of Biomedical and Healthcare Science 5:181-184.
• [15] Anosike J.C., Njoku A.J., Nwoke B.E.S., Ajero C.M.U., Osagiede U.R., Okoro O., Nwosu D.C. 2002. Epidemiology of urinary schistosomiasis in Ebonyi State, Nigeria. International Journal of Environmental Health and Human Development 3: 59-65.
• [16] Mafiana C.F., Ekpo U.F., Ojo D.A. 2003. Urinary schistosomiasis in preschool children in settlements around Oyan Reservoir in Ogun State, Nigeria: implications for control. Tropical Medicine and International Health 8: 78-82. doi:10.1046/j.1365-3156.2003.00988.x
• [17] Ogbeide O., Okojie O., Wagbatsoma V., Isah E. 1994. Schistosoma haematobium in rural school children in Nigeria. West African Journal of Medicine 13: 31-33.
• [18] Ugbomoiko U.S., Ofoezie I.E., Okoye I.C., Heukelbach J. 2010. Factors associated with urinary schistosomiasis in two peri-urban communities in south-western Nigeria. Annals of Tropical Medicine and Parasitology 104: 409-419. http://dx.doi.org/10.1179/136485910x12743554760469
• [19] Picquet M., Ernould J.C., Vercruysse J., Southgate V.R., Mbaye A., Sambou B., Niang M., Rollinson D. 1996. The epidemiology of human schistosomiasis in the Senegal river basin. Transactions of the Royal Society of Tropical Medicine and Hygiene 90: 340- 346. https://doi.org/10.1016/s0035-9203(96)90501-5
• [20] Lwambo N.J.S., Siza J.E., Brooker S., Bundy D.A.P., Guyatt H. 1999. Patterns of concurrent hookworm infection and schistosomiasis in schoolchildren in Tanzania. Transactions of the Royal Society of Tropical Medicine and Hygiene 93: 497-502. https://doi.org/10.1016/S0035-9203(99)90349-8
• [21] Hagan P. 1992. Reinfection, exposure and immunity in human schistosomiasis. Parasitology Today 8:12-16. http://dx.doi.org/10.1016/0169-4758(92)90303-J
• [22] Marchler-Bauer A., Lu S., Anderson J.B., Chitsaz F., Derbyshire M.K., DeWeese-Scott C., Fong J.H., Geer L.Y., Geer R.C., Gonzales N.R., Gwadz M., Hurwitz D.I., Jackson J.D., Ke Z., Lanczycki C.J., Lu F., Marchler G.H., Mullokandov M., Omelchenko M.V., Robertson C.L., Song J.S., Thanki N., Yamashita R.A., Zhang D., Zhang N., Zheng C., Bryant S.H. 2011. CDD: a Conserved Domain Database for the functional annotation of proteins. Nucleic Acids Research 39 (database issue): D225-D229. doi:10.1093/nar/gkq1189
• [23] Le T.H., Blair D., McManus D.P. 2000. Mitochondrial genomes of human helminths and their use as markers in population genetics and phylogeny. Acta Tropica 77: 243-256. http://doi.org/10.1016/s0001-706x(00)00157-1
• [24] Garba A., Barkiré N., Djibo A., Lamine M.S., Sofo B., Gouvras A.N., Bosqué-Oliva E., Webster J.P., Stothard J.R., Utzinger J., Fenwick A. 2010. Schistosomiasis in infants and preschool-aged children: infection in a single Schistosoma haematobium and a mixed S. haematobium-S. man soni foci of Niger. Acta Tropica 115: 212-219. http://doi.org/10.1016/j.actatropica.2010.03.005

Identyfikatory

DOI

10.17420/ap6302.97