EN
The aim of this study was identification features of cultivation representatives of genus Acanthamoeba isolated from bentonite using Cellulosimicrobium sp. as a bacteria-feeders. Identification of isolated bacteria was conducted by morphological, cultural and molecular-genetic methods. The cultivation of free-living „bentonite” amoeba on the lawn of Cellulosimicrobium sp. have gained significant advantages than using Escherichia coli as a bacteriafeeders was shown. “Bentonite” amoeba form crateroid plaques, which fit to the quantitative characteristic materials which contains amoeba, during deep co-cultivation Acanthamoeba sp. and Cellulosimicrobium sp. on 1% glucose meetpeptone agar.