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2015 | 37 | 01 |
Tytuł artykułu

ATG5 is required to limit cell death induced by Pseudomonas syringae in Arabidopsis and may be mediated by the salicylic acid pathway

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Autophagy can be regarded as a protection mechanism to restrict programmed cell death (PCD) induced by pathogen infection during plant innate immunity in the early stages. Autophagy related 5 (ATG5) plays an important role in autophagy in Arabidopsis. We investigated the function of ATG5 in Arabidopsis in the hypersensitive response (HR)-PCD elicited by both virulent and avirulent strains of Pseudomonas syringae pv. tomato bacteria DC3000. Results show that ATG5 plays a vital role in limiting HR induced by P. syringae strains and colocalizes with autophagic bodies during the early phase of bacterial infection. In addition, the P. syringae-induced response is mediated by the salicylic acid (SA) signaling pathway. In summary, ATG5 is required for limiting HRPCD induced in Arabidopsis by P. syringae strains and may be mediated by SA signaling.
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-
Rocznik
Tom
37
Numer
01
Opis fizyczny
Article: 1731 [11 p.], fig.,ref.
Twórcy
autor
  • MOE Key Laboratory of Laser Life Science, Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
autor
  • MOE Key Laboratory of Laser Life Science, Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
  • Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China
autor
  • MOE Key Laboratory of Laser Life Science, Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
autor
  • MOE Key Laboratory of Laser Life Science, Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
  • Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China
autor
  • MOE Key Laboratory of Laser Life Science, Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
Bibliografia
  • Bi YH, Chen WL, Zhang WN, Zhou Q, Yun LJ, Xing D (2009) Production of reactive oxygen species, impairment of photosynthetic function and dynamic changes in mitochondria are early events in cadmium induced cell death in Arabidopsis thaliana. Biol Cell 101:629–643
  • Bonas U, Lahaye T (2002) Plant disease resistance triggered by pathogen-derived molecules: refined models of specific recognition. Curr Opin Microbiol 5:44–50
  • Bonfig KB, Schreiber U, Gabler A, Roitsch T, Berger S (2006) Infection with virulent and avirulent P. syringae strains differentially affects photosynthesis and sink metabolism in Arabidopsis leaves. Planta 225:1–12
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
  • Chakraborti T, Das S, Mondal M, Roychoudhury S, Chakraborti S (1999) Oxidant, mitochondria and calcium: an overview. Cell Signal 11:77–85
  • Chen ZY, Kloek AP, Cuzick A, Moeder W, Tang DZ, Innes RW, Klessig DF, McDowell JM, Kunkel BN (2004) The Pseudomonas syringae type III effector AvrRpt2 functions downstream or independently of SA to promote virulence on Arabidopsis thaliana. Plant J 37:494–504
  • Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
  • Dangl JL, Jones JD (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833
  • Doelling JH, Walker JM, Friedman EM, Thompson AR, Vierstra RD (2002) The APG8/12-activating enzyme APG7 is required for proper nutrient recycling and senescence in Arabidopsis thaliana. J Biol Chem 277:33105–33114
  • Dong J, Chen W (2013) The role of autophagy in chloroplast degradation and chlorophagy in immune defenses during PstDC3000 (AvrRps4) infection. PLoS One 8(8):e73091
  • Gómez-Gómez L, Boller T (2002) Flagellin perception: a paradigm for innate immunity. Trends Plant Sci 7:251–256
  • Hatsugai N, Kuroyanagi M, Yamada K, Meshi T, Tsuda S, Kondo M, Nishimura M, Hara-Nishimura I (2004) A plant vacuolar protease, VPE, mediates virus-induced hypersensitive cell death. Science 305:855–858
  • Hofius D, Schultz-Larsen T, Joensen J, Tsitsigiannis DI, Petersen NHT, Mattsson O, Jorgensen LB, Jones JDG, Mundy J, Petersen M (2009) Autophagic components contribute to hypersensitive cell death in Arabidopsis. Cell 137:773–783
  • Kariola T, Brader G, Li J, Palva ET (2005) Chlorophyllase 1, a damage control enzyme, affects the balance between defense pathways in plants. Plant Cell 517:282–294
  • Katagiri F, Thilmony R, He SY (2002) The Arabidopsis thaliana–Pseudomonas syringae interaction. In: Somerville CR, Meyerowitz EM (eds) The Arabidopsis book. American Society of Plant Biologists, Rockville, pp 1–39
  • King EO, Ward NK, Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44:301–307
  • Kloek AP, Verbsky ML, Sharma SB, Schoelz JE, Vogel J, Klessig DF, Kunkel BN (2001) Resistance to Pseudomonas syringae conferred by an Arabidopsis thaliana coronatine-insensitive (coi1) mutation occurs through two distinct mechanisms. Plant J 26:509–522
  • Koch E, Slusarenko A (1990) Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell 2:437–445
  • Kunkel BN, Bent AF, Dahlbeck D, Innes RW, Staskawicz BJ (1993) RPS2, an Arabidopsis disease resistance locus specifying recognition of Pseudomonas syringae strains expressing the avirulence gene avrRpt2. Plant Cell 5:865–875
  • Mauch-Mani B, Slusarenko AJ (1996) Production of salicylic acid precursors is a major function of phenylalanine ammonialyase in the resistance of Arabidopsis to Peronospora parasitica. Plant Cell 8:203–212
  • Nuria SC, Dominique V, Andrea S, Charles C, Frank VB, Jeffery LD, Petra E (2010) Arabidopsis type I metacaspases control cell death. Science 330:1393–1397
  • Penninckx IAMA, Thomma BPHJ, Buchala A, Metraux JP, Broekaert WF (1998) Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis. Plant Cell 10:2103–2113
  • Seay M, Patel S, Dinesh-Kumar SP (2006) Autophagy and plant innate immunity. Cell Microbiol 8:899–906
  • Straus MR, Rietz S, Themaat EVLV, Bartsch M, Parker JE (2010) Salicylic acid antagonism of EDS1-driven cell death is important for immune and oxidative stress responses in Arabidopsis. Plant J 62:628–640
  • Tao Y, Xie Z, Chen W, Glazebrook J, Chang HS, Han B, Zhu T, Zou GZ, Katagiri F (2003) Quantitative nature of Arabidopsis responses during compatible and incompatible interactions with the bacterial pathogen Pseudomonas syringae. Plant Cell 15:317–330
  • Thomma BPHJ, Eggermont K, Penninckx IAMA, Mauch-Mani B, Vogelsang R, Cammue BPA, Broekaert WF (1998) Separate jasmonate-dependent and salicylate-dependent defense-response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc Natl Acad Sci 95:15107–15111
  • Yoshimoto K, Jikumaru Y, Kamiya Y, Kusano M, Consonni C, Panstruga R, Ohsumi Y, Shirasu K (2009) Autophagy negatively regulates cell death by controlling NPR1-dependent salicylic acid signaling during senescence and the innate immune response in Arabidopsis. Plant Cell 21:2914–2927
  • Yun LJ, Chen WL (2011) SA and ROS are involved in methyl salicylate-induced programmed cell death in Arabidopsis thaliana. Plant Cell Rep 30:1231–1239
  • Zhang WN, Chen WL (2011) Role of salicylic acid in alleviating photochemical damage and autophagic cell death induction of cadmium stress in Arabidopsis thaliana. Photochem Photobiol Sci 10:947–955
  • Zhang LR, Xing D (2008) Methyl jasmonate induces production of reactive oxygen species and alterations in mitochondrial dynamics that precede photosynthetic dysfunction and subsequent cell death. Plant Cell Physiol 49:1092–1111
  • Zhang LR, Xu QX, Xing D, Gao CJ, Xiong HW (2009) Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure. Plant Physiol 150:1773–1783
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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