PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 09 |

Tytuł artykułu

In vitro propagation and the acclimatization effect on the synthesis of 2-hydroxy-4-methoxy benzaldehyde in Decalepis hamiltonii Wight and Arn.

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The present study reports a high frequency in vitro propagation protocol through apical bud sprouting and basal organogenic nodule formation in shoot tip explants of Decalepis hamiltonii, an endemic and endangered medicinal liana. Among different combinations of plant growth regulators (PGRs) and growth additives, maximum of 8.20 shoots per explant with mean shoot length of 6.54 cm were induced on Murashige and Skoog’s medium (MS) supplemented with 5.0 µM 6-benzyladenine (BA) + 0.5 µM indole-3-acetic acid (IAA) + 30.0 µM adenine sulphate (ADS) through apical bud sprouting. On single cytokinin treatment explants did not exhibit good multiplication but showed nodulation (N₁) from the basal cut end similar to cytokinin–auxin combination (N₂). Between two types of nodular tissues, N₂ was proved to be better for maximum shoot regeneration (15.40 shoots per explant) and shoot length (4.56 cm) when cultured on MS medium supplemented with 5.0 µM BA, 0.5 µM IAA, 30.0 µM ADS and 1.0 µM gibberellic acid (GA₃). Microshoots were efficiently rooted on half-strength MS medium supplemented with 2.5 µM α-naphthalene acetic acid (NAA). After successful acclimatization in Soilrite, 95.10 % plantlets were survived in field conditions. Histological investigation proved useful in ascertaining the callogenic nature of the regenerating nodular tissue formed at the basal cut end of shoot tip explant. Acclimatized plantlets were studied for the estimation of chlorophyll and carotenoid content as well as the net photosynthetic rate (PN) during subsequent days of transfer to ex vitro condition. Moreover, acclimatization had a significant effect on biomass production and the synthesis of 2-hydroxy-4-methoxy benzaldehyde (2HMB). Maximum fresh weight (3.78 gm/plant), dry weight (0.39 gm/plant) of roots and 2HMB content (15.94 µg/ml of extract) were noticed after 8 weeks of acclimatization.

Wydawca

-

Rocznik

Tom

36

Numer

09

Opis fizyczny

p.2331-2344,fig.,ref.

Twórcy

autor
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202 002 Aligarh, UP, India
autor
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, 202 002 Aligarh, UP, India
autor
  • Department of Botany, Faculty of Science, Jamia Hamdard (Hamdard University), 110 062 New Delhi, India
autor
  • Department of Botany, Faculty of Science, Jamia Hamdard (Hamdard University), 110 062 New Delhi, India

Bibliografia

  • Ahmad N, Faisal M, Anis M, Aref IM (2010) In vitro callus induction and plant regeneration from leaf explants of Ruta graveolens L. South African J Bot 76:597–600. doi:10.1016/j.sajb.2010.03.008
  • Aly HMA, Hattori K (2007) Histological observations on plant regeneration in Faba bean cotyledon (Vicia faba L.) cultured in vitro. Asian J Plant Sci 6:723–731. doi:10.3923/ajps.2007.723.731
  • Anitha S, Pullaiah T (2002) In vitro propagation of Decalepis hamiltonii. J Trop Med Plants 3:227–232
  • Anonymous (2003) The wealth of India: A dictionary of Indian raw materials and industrial products. CSIR, New Delhi, Vol 3, p 24
  • Bais HP, George J, Ravishankar GA (2000a) In vitro propagation of Decalepis hamiltonii Wight & Arn. an endangered shrub through axillary bud cultures. Curr Sci 79:408–410
  • Bais HP, Sudha G, Suresh B, Ravishankar GA (2000b) AgNO₃ influences in vitro root formation in Decalepis hamiltonii Wight & Arn. Curr Sci 79:894–898
  • Datta MM, Mukherjee P, Ghosh B, Jha TB (2007) In vitro clonal propagation of biodiesel plant Jatropha curcas L. Curr Sci 93:1438–1442
  • George J, Ravishankar GA, Keshava N, Udhayashankar K (1999) Antibacterial activity of super critical extract from Decalepis hamiltonii roots. Fitoterapia 70:172–174. doi:10.1016/S0367-326X(98)00037-9
  • George J, Bais HP, Ravishankar GA (2000) Optimization of media constituents for shoot regeneration from leaf callus cultures of Decalepis hamiltonii Wight & Arn. HortScience 35:296–299
  • Giridhar P, Vijaya Ramu D, Reddy BO, Rajasekaran T, Ravishankar GA (2003) Influence of phenylacetic acid on clonal propagation of Decalepis hamiltonii Wight and Arn.; an endangered shrub. In Vitro Cell Dev Biol-Plant 39:463–467. doi:10.1079/IVP2003448
  • Giridhar P, Kumar V, Ravishankar GA (2004a) Somatic embryogenesis, organogenesis and regeneration from leaf callus culture of Decalepis hamiltonii Wight & Arn., an endangered shrub. In Vitro Cell Dev Biol-Plant 40:567–571. doi:10.1079/IVP2004567
  • Giridhar P, Rajasekaran T, Nagarajan S, Ravishankar GA (2004b) Production of 2-hydroxy-4-methoxy benzaldehyde in roots of tissue culture raised and acclimatized plants of Decalepis hamiltonii Wight & Arn an endangered shrub endemic to Southern India and evaluation of its performance vis-à-vis plants from natural habitat. Indian J Exp Biol 42:106–110
  • Giridhar P, Gururaj HB, Ravishankar GA (2005a) In vitro shoot multiplication through shoot tip cultures of Decalepis hamiltonii Wight & Arn, a threatened plant endemic to southern India. In Vitro Cell Dev Biol-Plant 41:77–80. doi:10.1079/IVP2004600
  • Giridhar P, Rajasekaran T, Ravishankar GA (2005b) Production of root specific flavour compound, 2-hydroxy-4-methoxy benzaldehyde by normal root cultures of Decalepis hamiltonii Wight & Arn (Asclepiadaceae). J Sci Food Agric 85:61–64
  • Husain MK, Anis M (2009) Rapid in vitro multiplication of Melia azedarach L. (a multipurpose woody tree). Acta Physiol Plant 31:765–772. doi:10.1007/s11738-009-0290-7
  • Husain MK, Anis M, Shahzad A (2008) In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants. Acta Physiol Plant 30:353–359. doi:10.1007/s11627-006-9011-8
  • Jacob KC (1937) An unrecorded economic product Decalepis hamiltonii W. & Arn., family Asclepiadaceae. Madras Agric J 25:176
  • Karuppusamy S, Kiranmai C, Aruna V, Pulliah T (2009) In vitro conservation of Ceropegia intermedia—an endemic plant of south India. African J Biotechnol 8:4052–4057
  • Kaur K, Kanta U (2000) Clonal propagation of Acacia catechu Willd. by shoot tip culture. Plant Growth Reg 31:143–145. doi:10.1023/A:1006362318265
  • Khan MI, Ahmad N, Anis M (2011) The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance. Trees 25:577–584. doi:10.1007/s00468-010-0534-6
  • Kumar V, Sharma A, Chayapathy B, Prasad N, Gururaj HB, Giridhar P, Ravishankar GA (2007) Direct shoot bud induction and plant regeneration in Capsicum frutescens Mill.: influence of polyamines and polarity. Acta Physiol Plant 29:11–18. doi:10.1007/s11738-006-0002-5
  • Malik SK, Chaudhury R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Sci Hort 106:539–553. doi:10.1016/j.scienta.2005.05.002
  • Misra P, Chakrabarty D (2009) Clonal propagation of Rosa clinophylla Thory. through axillary bud culture. Sci Hort 119:212–216. doi:10.1016/j.scienta.2008.07.028
  • Murashige T (1974) Plant propagation through tissue culture. Annu Rev Plant Physiol 25:135–166. doi:10.1146/annurev.pp.25.060174.001031
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–494. doi:10.1111/j.1399-3054.1962.tb08052.x
  • Murti PB, Seshadri TR (1941) A study of the chemical components of the roots of Decalepis hamiltonii (Makali veru). Part IV—Resinol of Decalepis hamiltonii and Hemidesmus indicus. Proc Ind Acad Sci A 14:93–99. doi:10.1007/BF03046552
  • Nagarajan SL, Rao LJM, Gurudatta KN (2001) Chemical composition of the volatiles of Decalepis hamiltonii Wight & Arn. Flavour Frag J 16:27–29
  • Nikam TD, Savant RS (2009) Multiple shoot regeneration and alkaloid cerpegin accumulation in callus culture of Ceropegia juncea Roxb. Physiol Mol Biol Plants 15:71–77. doi:10.1007/s12298-009-0007-9
  • Nikolić R, Mitić N, Ninković S, Vinterhalter B, Zdravković-Korać S, Nešković M (2010) Gibberellic acid promotes in vitro regeneration and shoot multiplication in Lotus corniculatus L. Plant Growth Reg 62:181–188. doi:10.1007/s10725-010-9505-6
  • Parimalan R, Giridhar P, Gururaj HB, Ravishankar GA (2009) Micropropagation of Bixa orellana using phytohormones and triacontanol. Biol Plant 53:347–350. doi:10.1007/s10535-009-0064-5
  • Phadke NY, Gholap AS, Ramakrishnan K, Subbulakshmi G (1994) Essential oil of Decalepis hamiltonii as an antimicrobial agent. J Food Sci Technol 31:472–475
  • Preece JE, Sutter EG (1991) Acclimatization of micropropagated plants to the greenhouse and field. In: Debergh PC, Zimmerman RH (eds) Micropropagation: Technology and Application. Kluwer Academic Publishers, Dordrecht, pp 71–93. doi:10.1007/978-94-009-2075-0-5
  • Raju AJS (2010) Pollination biology of Decalepis hamiltonii and Shorea tumbuggai. Lambert Academic Publishing, Germany
  • Reddy OB, Giridhar P, Ravishankar GA (2001) In vitro rooting of Decalepis hamiltonii Wight & Arn., an endangered shrub, by auxins and root promoting agents. Curr Sci 81:1479–1482
  • Reddy OB, Giridhar P, Ravishankar GA (2002) The effect of triacontanol on micropropagation of Capsicum frutescens and Decalepis hamiltonii W & A. Plant Cell Tiss Org Cult 71:253–258. doi:10.1023/A:1020342127386
  • Sahai A, Shahzad A (2013) High frequency in vitro regeneration system for conservation of Coleus forsklohlii: a threatened medicinal herb. Acta Physiol Plant 35:473–481. doi:10.1007/s11738-012-1090-z
  • Sahai A, Shahzad A, Sharma S (2010) Histology of organogenesis and somatic embryogenesis in excised root cultures of an endangered species Tylophora indica (Asclepiadaceae). Aust J Bot 58:198–205. doi:10.1071/BT09220
  • Senapati SK, Aparajita S, Rout GR (2013) Micropropagation and assessment of genetic stability in Celastrus paniculatus: an endangered medicinal plant. Biology 68:627–632. doi:10.2478/s11756-013-0187-1
  • Sharma S, Shahzad A (2012) Encapsulation technology for short-term storage and conservation of a woody climber, Decalepis hamiltonii Wight and Arn. Plant Cell Tiss Org Cult 111:191–198. doi:10.1007/s11240-012-0183-0
  • Sopher CR, Krol M, Huner NPA, Moore AE, Fletcher RA (1999) Chloroplastic changes associated with paclobutrazol induced stress protection in maize seedlings. Can J Bot 7:279–290
  • Sudha CG, Seeni S (2001) Establishment and analysis of fast-growing normal root culture of Decalepis arayalpathra, a rare endemic medicinal plant. Curr Sci 81:371–374
  • Thiyagarajan M, Venkatachalam P (2013) A reproducible and high frequency plant regeneration from mature axillary node explants of Gymnema sylvestre (Gurmur)-an important antidiabetic endangered medicinal plant. Indus Crop Prod 50:517–524. doi:10.1016/j.indcrop.2013.08.027
  • Ugraiah A, Karuppusamy S, Pullaiah T (2010) Micropropagation of Marsdenia brunoniana Wight & Arn.—a rare antidiabetic plant. Plant Tissue Cult Biotech 20:7–12. doi:10.3329/ptcb.v20i1.5958
  • Van Huylebroeck JM, Piqueras A, Debergh PC (2000) The evolution of photosynthetic capacity and the antioxidant enzymatic system during acclimatization of micropropagated Calathea plants. Plant Sci 155:59–66. doi:10.1016/S0168-9452(00)00201-6

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-1779114e-fde1-47a6-9bde-807574d4bb43
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.