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2010 | 13 | 4 |
Tytuł artykułu

Practical aspects of immunocytochemistry in canine lymphomas

Autorzy
Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to evaluate the utility of immunocytochemistry in a standard veterinary practice and to determine the immunophenotype of tumor cells in cases of multicentric lymphoma in dogs by immunocytochemical analysis of fine-needle biopsy specimens. The study was performed on cytological samples collected from 54 dogs, in which multicentric lymphoma was recognised based on clinical data, cytology or cytology and histology, and follow-up information. Diagnosis of lymphoma was established according to the updated Kiel classification. Immunocytochemical assays were conducted using commercially available antibodies to the pan T-lymphocyte marker CD3 and B cell antigen receptor complex CD79 alpha. Among all animals examined B cell lymphoma was recognized in 42/54 (77.8%) of cases, while in the remaining 12/54 (22.2%) of dogs T cell lymphoma was recognized. In 11 animals with lymphoma recognized cytologically, in which an entire lymph node was obtained for histology, the results of routine cytology and immunocytochemistry fully corresponded with findings revealed by histology and immunohistochemistry. Immunocytochemistry can be successfully conducted in smears stored at room temperature for 24 hours without changes of staining results. It can be stated that application of standard cytopathological assessment in connection with immunocytochemistry of lymph nodes samples collected from dogs with lymphoma is a method of choice for establishing final diagnosis, and avoids the need for reexamination or collection of tissue samples for histopathology and immunohistochemistry during surgical procedures in ambiguous cases.
Wydawca
-
Rocznik
Tom
13
Numer
4
Opis fizyczny
p.661-668,fig.,ref.
Twórcy
  • Department of Pathology and Veterinary Diagnostic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159c, 02-766 Warsaw, Poland
Bibliografia
  • Caniatti M, Roccabianca P, Scanziani E, Paltrinieri S, Moore PF (1996) Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy. Vet Pathol 33: 204-212.
  • Das DK (1999) Value and limitations of fine-needle aspiration cytology in diagnosis and classification of lymphomas. A review. Diagn Cytopathol 21: 240-249.
  • Dzimira S (2007) Immunocytochemical and cytomorphometric diagnostics of malignant lymphomas in dogs. Bull Vet Inst Pulawy 51: 71-78.
  • Fisher DJ, Naydan D, Werner LL, Moore PF (1995) Immunophenotyping lymphomas in dogs: a comparison of results from fine needle aspirate and needle biopsy samples. Vet Clin Pathol 24: 118-123.
  • Fournel-Fleury C, Magnol JP, Bricaire P, Marchal T, Chabanne L, Delverdier A, Bryon PA, Felman P (1997) Cytohistological and immunological classification of canine malignant lymphomas: comparison with human non-Hodgkin’s lymphomas. J Comp Pathol 117: 35-59.
  • Fournel-Fleury C, Ponce F, Felman P, Blavier A, Bonnefont C, Chabanne L, Marchal T, Cadore JL, Goy-Thollot I, Ledieu D, Ghernati I, Magnol JP (2002) Canine T-cell lymphomas: a morphological, immunological, and clinical study of 46 new cases. Vet Pathol 39: 92-109.
  • Gibson D, Aubert I, Woods JP, Abrams-Ogg A, Kruth S, Wood RD, Bienzle D (2004) Flow cytometric immunophenotype of canine lymph node aspirates. J Vet Intern Med 18: 710-717.
  • Guija de Arespacochaga A, Schwendenwein I, Weissenbock H (2007) Retrospective study of 82 cases canine lymphoma in Austria based on the Working Formulation and immunophenotyping. J Comp Pathol 136: 186-192.
  • Jagielski D, Lechowski R, Hoffman-Jagielska M, Winiarczyk S (2002) A retrospective study of the incidence and prognostic factors of multicentric lymphoma in dogs (1998-2000). J Vet Med A 49: 419-424.
  • Jubala CM, Wojcieszyn JW, Valli VE, Getzy DM, Fosmire SP, Coffey D, Bellgrau D, Modiano JF (2005) CD20 expression in normal canine B cells and in canine non-Hodgkin lymphoma. Vet Pathol 42: 468-476.
  • Lana S, Plaza S, Hampe K, Burnett R, Avery AC (2006) Diagnosis of mediastinal masses in dogs by flow cytometry. J Vet Intern Med 20: 1161-1165.
  • Pastor M, Chalvet-Monfray K, Marchal T, Keck G, Magnol JP, Fournel-Fleury C, Ponce F (2009) Genetic and environmental risk indicators in canine non-Hodgkin's lymphomas: breed association and geographic distribution of 608 cases diagnosed throughout France over 1 year. J Vet Intern Med 23: 301-310.
  • Ponce F, Magnol JP, Ledieu D, Marchal T, Turinelli V, Chalvet-Monfray K, Fournel-Fleury C (2004) Prognostic significance of morphological subtypes in canine malignant lymphomas during chemotherapy. Vet J 167: 158-166.
  • Sapierzyński R, Micuń J (2009) Lymphadenomegaly in dogs – cytological study. Pol J Vet Sci 12: 263-268.
  • Sokolowska J, Cywińska A, Malicka E (2005) p53 expression in canine lymphoma. J Vet Med A 52: 172-175.
  • Sozmen M, Tasca S, Carli E, De Lorenzi D, Furlanello T, Caldin M (2005) Use of fine needle aspirates and flow cytometry for the diagnosis, classification, and immunophenotyping of canine lymphomas. J Vet Diagn Invest 17: 323-330.
  • Sueiro FA, Alessi AC, Vassallo J (2004) Canine lymphomas: a morphological and immunohistochemical study of 55 cases, with observations on p53 immunoexpression. J Comp Pathol 131: 207-213.
  • Teske E, van Heerde P (1996) Diagnostic value and reproducibility of fine-needle aspiration cytology in canine malignant lymphoma. Vet Q 18: 112-115.
  • Vail DM, Kravis LD, Kisseberth WC, Ogilvie GK, Volk LM (1997) Application of rapid CD3 immunophenotype analysis and argyrophilic nucleolar organizer region (Ag-NOR) frequency to fine needle aspirate specimens from dogs with lymphoma. Vet Clin Pathol 26: 66-69.
  • Williams MJ, Avery AC, Lana SE, Hillers KR, Bachand AM, Avery PR (2008) Canine lymphoproliferative disease characterized by lymphocytosis: immunophenoptypic markers of prognosis. J Vet Intern Med 22: 596-601.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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