Effect of storage conditions on antioxidant activity of bee pollen extracts

• Autorzy: Rzepecka-Stojko A. , Drzal A. , Stojko J. , Buszman E.
• Języki publikacji: EN

Abstrakty

EN

Bee pollen is a product of rich and varied chemical composition, and its biological activities are diverse. Many of these activities are related to the antioxidant effect of bee pollen. The aim of this study was to determine the effect of storage conditions on the antioxidant activity of bee pollen extracts. The study was conducted on three types of bee pollen extracts, namely, ethanol and pepsin extracts of bee pollen, as well as on ethanol extracts of pepsin-digested bee pollen. Antioxidant activity was determined by a DPPH method, directly after obtaining the extracts and after storing them for twelve months under various conditions, i.e. at –18°C in the dark, at 4-8°C in the dark, at room temperature in the dark, and at room temperature in the light. It was concluded that the 12-month storage of bee pollen extracts caused a decrease in the antioxidant activity of all extracts examined, and the decrease depended on storage conditions. The highest decrease in antioxidant activity was observed in all types of extracts stored at room temperature in the light. The lowest decrease in antioxidant activity was found in ethanol extracts of pepsin-digested bee pollen.

Słowa kluczowe

EN

storage condition; antioxidant activity; bee; pollen extract; free radical; bee pollen

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2014
• Tom: 70
• Numer: 12
• Opis fizyczny: p.740-744,fig.,ref.

Twórcy

autor

Rzepecka-Stojko A.

• Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jagiellonska 4, 41-200 Sosnowiec, Poland

autor

Drzal A.

• Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jagiellonska 4, 41-200 Sosnowiec, Poland

autor

Stojko J.

• Department of Hygiene, Bioanalysis and Environmental Studies, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Kasztanowa 3A, 41-200 Sosnowiec, Poland

autor

Buszman E.

• Department of Pharmaceutical Chemistry, School of Pharmacy with the Division of Laboratory Medicine, Medical University of Silesia, Jagiellonska 4, 41-200 Sosnowiec, Poland

Bibliografia

• 1. Almaraz-Abarca N., Campos M. G., Avila-Reyes J. A., Naranjo-Jimenez N., Herrera-Carrol J., Gonzalez-Valdez L. S.: Antioxidant activity of polyphenolic extract of monofloral honeybee-collected pollen from mesquite (Prosopis juliflora, Leguminosae). J. Food Compos. Anal. 2007, 20(2), 119-124.
• 2. Almaraz-Abarca N., Campos M. G., Avila-Reyes J. A., Naranjo-Jimenez N., Herrera-Corral J., Gonzales-Valdez L. S.: Variability of antioxidant activity among honeybee-collected pollen of different botanical origin. Interciencia 2004, 29 (10), 574-578.
• 3. Almeida-Muradian L. B., Pamplona L. C., Coimbra S., Barth O. M.: Chemical composition and botanical evaluation of dried bee pollen pellets. J. Food Compos. Anal. 2005, 18(1), 105-111.
• 4. Baltrusaityte V., Venscutonis P. R., Ceksteryte V.: Radical scavenging activity of different floral origin honey and beebread phenolic extracts. Food Chem. 2007, 101(2), 502-514.
• 5. Bogdanov S.: Quality and standards of pollen and beeswax. Apiacta. 2004, 38, 334-341.
• 6. Brand-Williams W., Culivier M. E., Berest C.: Use of a free radical method to evaluate antioxidant activity. Lebensm. – Wiss. u. – Technol. 1995, 28(1), 25-30.
• 7. Campos M. G., Webby R. F., Markham K. R., Mitchell K. A. Cuhna A. P.: Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids. J. Agric. Food Chem. 2003, 51, 742-745.
• 8. Carpes S. T., Mourao G. B., Alencar S. M., Masson M. L.: Chemical composition and free radical scavenging activity of Apis mellifera bee pollen from Southern Brazil. Braz. J. Food Technol. 2009, 12(3), 220-229.
• 9. Eraslan G., Kanbur M., Silici S., Liman B. C., Altinordulu S., Sarica Z. S.: Evaluation of protective effect of bee pollen against propoxur toxicity in rat. Ecotoxicol. Environ. Saf. 2009, 72, 931-937.
• 10. Feas X., Vazquez-Tato M. P., Estevinho L., Seijas J. A., Iglesias A.: Organic bee pollen: Botanical origin, nutritional value, bioactive compounds, antioxidant activity and microbiological quality. Molecules 2012, 17(7), 8359-8377.
• 11. Graikou K., Kapeta S., Aligiannis N., Sotiroudis G., Chondrogianni N., Gonos E., Chinou I.: Chemical analysis of Greek pollen – Antioxidant, antimicrobial, and proteasome activation properties. Chem. Cent. J. 2011, 5:33 http://journal.chemistrycentral.com/content/5/1/33.
• 12. Gulcin I.: Antioxidant activity of food constituents: an overview. Arch. Toxicol. 2012, 86, 345-391.
• 13. Kroyer G., Hegedus N.: Evaluation of bioactive properties of pollen extracts as functional dietary food supplement. Innov. Food Sci. Emerg. Technol. 2001, 2, 171-174.
• 14. Marghitas L. A., Stanciu O. G., Dezmirean D. S., Bobis O., Popescu O., Bogdanov S., Campos M. G.: In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem. 2009, 115, 878-883.
• 15. Martins M. C. T., Morgano M. A., Vicente E., Baggio S. R., Rodriguez-Amaya D. B.: Physicochemical composition of bee pollen from eleven Brazilian states. J. Apic. Sci. 2011, 55(2), 107-116.
• 16. Morais M., Moreira L., Feas X., Estevinho L. M.: Honeybee-collected pollen from five Portuguese natural parks: palynological origin, phenolic content, antioxidant properties and antimicrobial activity. Food Chem. Toxicol. 2011, 49, 1096-1101.
• 17. Nagai T., Inoue R., Suzuki, N., Myoda, T., Nagashima T.: Antioxidative ability in a linoleic acid oxidation system and scavenging abilities against active oxygen species of enzymatic hydrolysates from pollen Cistus ladaniferus. Int. J. Mol. Med. 2005, 15, 259-263.
• 18. Rzepecka-Stojko A., Maciejewska-Paszek I., Stec M., Kurzeja E., Kęska A., Pawłowska-Góral K.: The influence of extraction method on obtaining polyphenolic compounds from bee pollen. Farm. Przegl. Nauk. 2010, 1, 38-41.
• 19. Rzepecka-Stojko A., Pilawa B., Ramos P., Stojko J.: Antioxidative properties of bee pollen extracts examined by EPR spectroscopy. J. Apic. Sci. 2012, 56(1), 23-31.
• 20. Rzepecka-Stojko A., Stec M., Kurzeja E., Gawrońska E., Pawłowska-Góral K.: The effect of storage of bee pollen extracts on the polyphenol content. Pol. J. Environ. Stud. 2012, 21(4), 1007-1111.
• 21. Silva T. M. S., Camara C. A., Lins A. C. S., Barbosa-Filho J. M., Silva E. M. S., Freitas B. M., Santos F. A. R.: Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. J. Food Compos. Anal. 2006, 19, 507-511.
• 22. Szczęsna T.: Concentration of selected elements in honeybee-collected pollen. J. Apic. Sci. 2007, 51(1), 5-13.
• 23. Szczęsna T.: Long chain fatty acids composition of honeybee-collected pollen. J. Apic. Sci. 2006, 50(2), 65-79.
• 24. Szczęsna T.: Protein content and amino acid composition of bee-collected pollen from selected botanical origins. J. Apic. Sci. 2006, 50(2), 81-90.
• 25. Szczęsna T.: Protein content and amino acid composition of bee-collected pollen originating from Poland, South Korea and China. J. Apic Sci. 2006, 50(2), 91-99.
• 26. Szczęsna T.: Study on the sugar composition of honeybee-collected pollen. J. Apic. Sci. 2007, 51(1), 15-22.