Morphological characterization of biocontrol isolates of Trichoderma to study the correlation between morphological characters and biocontrol efficacy

• Autorzy: Kumar M. , Sharma P.
• Języki publikacji: EN

Abstrakty

EN

The morphological characterization was carried out for 5 isolates of Trichoderma harzianum and 7 isolates of Trichoderma viride and tested for their biocontrol efficacy. The isolates belonging to T.harzianum were analogous in colony colour, culture smell, mycelial colour, conidiation, conidial shape, conidial wall and conidial colour. Correspondingly the isolates of T.viride showed certain similarity in colony colour, colony edge, culture smell, conidiophore branching, conidial wall, conidial colour and chlamydospores. Inter specific differences through cluster analysis based on morphological characters grouped the twelve isolates into three major clusters where all the isolates of T.harzianum formed a single cluster while the isolates of T.viride were bifurcated into two groups. The clustering was substantiated by similarity index which showed maximum similarity among T.harzianum isolates with only less than 20% variation among themselves. Similarly the clusters having isolates of T.viride also had less variation within them. The biocontrol efficacy of these twelve isolates of Trichoderma was experimented by dual culture test under laboratory condition and there existed some relation between the biocontrol efficacy of these isolates and morphology.

Słowa kluczowe

EN

Trichoderma; fungal isolate; morphological characteristics; biological control; antagonistic activity; antagonistic effect

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2016
• Tom: 55
• Opis fizyczny: p.57-67,fig.,ref.

Twórcy

autor

Kumar M.

• Forest Wood Protection Division, Institute of Wood Science and Technology, Bangalore, 560003, India

autor

Sharma P.

• Division of Plant Pathology, Indian Agricultural Research Institute, Pusa, New Delhi, 1100012, India

Bibliografia

• [1] G.R. Bisby, Trichoderma viride Pers. ex Fries, and notes on Hypocrea, Trans. Brit. Mycol. Soc. 23 (1939) 149-168.
• [2] J. Bissett, A revision of the genus Trichoderma. I. Section Longibrachiatum sect, Nov. Can. J. Bot. 62 (1984) 924–931.
• [3] J. Bissett, A revision of the genus Trichoderma. II. Infrageneric classification, Can. J. Bot. 69 (1991a) 2357-2372.
• [4] J. Bissett, A revision of the genus Trichoderma. III. .Sect. Pachybasium, Can. J. Bot.69 (1991b) 2373-2417.
• [5] J. Bissett, A revision of the genus Trichoderma. IV. Additional notes on section Longibrachiatum. Can. J. Bot. 69 (1991c) 2418-2420.
• [6] J. Bissett, Trichoderma atroviride, Can. J. Bot. 70 (1992) 639-641.
• [7] C. Dennis and J. Webster, Antagonistic properties of species group of Trichoderma.1. Production of non-volatile antibiotics, Trans. Brit. Mycol. Soc. 57 (1971) 25-39.
• [8] E.L. Ghisalberti and K. Sivasithamparam, The role of secondary metabolites produced by Trichoderma species in biological control (abstract), Petria. 1 (1991) 130-131.
• [9] L. Hjeljord and A. Tronsmo, Trichoderma and Gliocladium in Biological Control: An Overview. In: Harman, G.E., Kubicek, C.P. (Eds.), Trichoderma and Gliocladium. Vol. 2. Enzymes, Biological Control and Commercial Applications. Taylor and Francis Ltd., London. (1998) 131-151pp.
• [10] J.A. Lewis and G.C. Papavizas, Integrated control of Rhizoctonia fruit rot of cucumber, Phytopathology. 70 (1980) 85-89.
• [11] E. Lieckfeldt G.J. Samuels, H.I. Helgard and O. Petrini A morphological and molecular perspective of Trichoderma viride: is it one or two species, Appl. Environ. Microbial. 65 (1999) 2418–2428.
• [12] F. Munaut, N. Hamaide and H. Maraite, Molecular and morphological characterization of Colletotrichum gloeosporioides from native Mexican Stylosanthes species, Pl. Path. 50 (2001) 383-396.
• [13] H.I. Nirenberg, Untersuchungen uber die morphologische und biologische differenzierung in der Fusarium Sektion Liseola, Mitt Biol Bundesanstalt fur Land-Forstw Berlin-Dallem. 169 (1976) 1-117.
• [14] M.A. Rifai, A revision of the genus Trichoderma, Mycol. Papers. (1969) 116: 1–56.
• [15] G.J. Samuels, S.L. Dodd, W. Gams, W, L.A. Castlebury, and O. Petrini, Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia, 94 (2002) 146-170.
• [16] P. Sharma, Induction of systemic resistance to downy mildew by exogenous application of plant activator, Annals plant Prot. Sci. 10 (1) (2002) 99-103.
• [17] P. Sharma, and P. Dureja, Evaluation of T.harzianum and T.viride isolates at BCA Pathogen Crop Interface, J. Mycol. Pl. Pathol. 34 (1) (2004) 47-55.
• [18] P. Sharma, and S.K. Sain, Development of suitable techniques for evaluating virulence and biocontrol activity of Trichoderma isolates, Indian J. Pl. Pathol. 21 (2003) 16-21.
• [19] P. Sharma, and S.K. Sain, Induction of systemic resistance in tomato and cauliflower by Trichoderma species against stalk rot pathogen. (Sclerotinia sclerotiorum), J. Biocontrol. 18 (1) (2004) 21-28.
• [20] P. Sharma, S.K. Sain and S. James, Compatibility Study of Trichoderma isolates with Fungicides against Damping-off of cauliflower and tomato caused by Pythium aphanidermatum, Pesticide Research Journal. 15 (2) (2003) 133-138.
• [21] A. Tronsmo and J. Ystaas, Biological control of Botrytis cinerea on apple, Plant Dis. 64 (1980) 1009.
• [22] R. Weinding, Studies on a lethal principle effective in the parasitic action of Trichoderma lignorum on Rhizoctonia solani and other soil fungi, Phytopatholo. 24 (1934) 1153-1179.
• DOI References
• [1] G.R. Bisby, Trichoderma viride Pers. ex Fries, and notes on Hypocrea, Trans. Brit. Mycol. Soc. 23 (1939) 149-168. 10.1016/s0007-1536(39)80020-1
• [2] J. Bissett, A revision of the genus Trichoderma. I. Section Longibrachiatum sect, Nov. Can. J. Bot. 62 (1984) 924-931. 10.1139/b84-131
• [3] J. Bissett, A revision of the genus Trichoderma. II. Infrageneric classification, Can. J. Bot. 69 (1991a) 2357-2372. 10.1139/b91-297
• [4] J. Bissett, A revision of the genus Trichoderma. III. . Sect. Pachybasium, Can. J. Bot. 69 (1991b) 2373- 2417. 10.1139/b91-298
• [5] J. Bissett, A revision of the genus Trichoderma. IV. Additional notes on section Longibrachiatum. Can. J. Bot. 69 (1991c) 2418-2420. 10.1139/b91-299
• [6] J. Bissett, Trichoderma atroviride, Can. J. Bot. 70 (1992) 639-641. 10.1139/b92-082
• [9] L. Hjeljord and A. Tronsmo, Trichoderma and Gliocladium in Biological Control: An Overview. In: Harman, G.E., Kubicek, C.P. (Eds. ), Trichoderma and Gliocladium. Vol. 2. Enzymes, Biological Control and Commercial Applications. Taylor and Francis Ltd., London. (1998). 10.1086/393543
• [10] J.A. Lewis and G.C. Papavizas, Integrated control of Rhizoctonia fruit rot of cucumber, Phytopathology. 70 (1980) 85-89. 10.1094/phyto-70-85
• [12] F. Munaut, N. Hamaide and H. Maraite, Molecular and morphological characterization of Colletotrichum gloeosporioides from native Mexican Stylosanthes species, Pl. Path. 50 (2001) 383-396. 10.1046/j.1365-3059.2001.00564.x
• [13] H.I. Nirenberg, Untersuchungen uber die morphologische und biologische differenzierung in der Fusarium Sektion Liseola, Mitt Biol Bundesanstalt fur Land-Forstw Berlin-Dallem. 169 (1976) 1-117. 10.2307/3758963
• [15] G.J. Samuels, S.L. Dodd, W. Gams, W, L.A. Castlebury, and O. Petrini, Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia, 94 (2002) 146-170. 10.2307/3761854
• [21] A. Tronsmo and J. Ystaas, Biological control of Botrytis cinerea on apple, Plant Dis. 64 (1980) 1009. 10.1094/pd-64-1009
• DOI References
• [1] G.R. Bisby, Trichoderma viride Pers. ex Fries, and notes on Hypocrea, Trans. Brit. Mycol. Soc. 23 (1939) 149-168. 10.1016/s0007-1536(39)80020-1
• [2] J. Bissett, A revision of the genus Trichoderma. I. Section Longibrachiatum sect, Nov. Can. J. Bot. 62 (1984) 924-931. 10.1139/b84-131
• [3] J. Bissett, A revision of the genus Trichoderma. II. Infrageneric classification, Can. J. Bot. 69 (1991a) 2357-2372. 10.1139/b91-297
• [4] J. Bissett, A revision of the genus Trichoderma. III. . Sect. Pachybasium, Can. J. Bot. 69 (1991b) 2373- 2417. 10.1139/b91-298
• [5] J. Bissett, A revision of the genus Trichoderma. IV. Additional notes on section Longibrachiatum. Can. J. Bot. 69 (1991c) 2418-2420. 10.1139/b91-299
• [6] J. Bissett, Trichoderma atroviride, Can. J. Bot. 70 (1992) 639-641. 10.1139/b92-082
• [9] L. Hjeljord and A. Tronsmo, Trichoderma and Gliocladium in Biological Control: An Overview. In: Harman, G.E., Kubicek, C.P. (Eds. ), Trichoderma and Gliocladium. Vol. 2. Enzymes, Biological Control and Commercial Applications. Taylor and Francis Ltd., London. (1998). 10.1086/393543
• [10] J.A. Lewis and G.C. Papavizas, Integrated control of Rhizoctonia fruit rot of cucumber, Phytopathology70 (1980) 85-89. 10.1094/phyto-70-85 [12] F. Munaut, N. Hamaide and H. Maraite, Molecular and morphological characterization of Colletotrichum gloeosporioides from native Mexican Stylosanthes species, Pl. Path. 50 (2001) 383-396. 10.1046/j.1365-3059.2001.00564.x
• [13] H.I. Nirenberg, Untersuchungen uber die morphologische und biologische differenzierung in der Fusarium Sektion Liseola, Mitt Biol Bundesanstalt fur Land-Forstw Berlin-Dallem. 169 (1976) 1-117. 10.2307/3758963
• [15] G.J. Samuels, S.L. Dodd, W. Gams, W, L.A. Castlebury, and O. Petrini, Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus. Mycologia, 94 (2002) 146-170. 10.2307/3761854
• [21] A. Tronsmo and J. Ystaas, Biological control of Botrytis cinerea on apple, Plant Dis. 64 (1980) 1009. 10.1094/pd-64-1009

Identyfikatory

DOI

10.18052/www.scipress.com/ILNS.55.57