Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 31 | 2 |
Tytuł artykułu

Control of pattern of regenerant differentiation and plantlet production from leaflet segments of Azadirachta indica A. Juss. (neem)

Treść / Zawartość
Warianty tytułu
Języki publikacji
A process with controlled pattern of regenerant differentiation from leaflet segments leading to production of cloned plants of a 40-year-old tree of Azadirachta indica was developed.Atwo-step procedure was adopted for containing intervening callusing during regenerant differentiation using modified Murashige and Skoog (MS) medium, where in the first step the explants were subjected to pulse treatments having higher concentration of 6-benzylaminopurine (BAP), while in the second step they were cultured in one-tenth of the initial concentrations of BAP. In the present case, simultaneous differentiation of two types of morphogenetic structures, that is, shoot buds and the meristematic nodules was observed. However, differentiation of higher number of desirable regenerants—the shoot buds and a few meristematic nodules, rather than vice-versa could be controlled by increasing both, the concentration of BAP in pulse treatment and the duration of pulse treatment. In the optimum treatment, where the explants were exposed to 8.88 µM BAP and 81.43 µM adenine hemisulphate for 5 days followed by their transfer to 0.88 µM BAP and 81.43 µM adenine hemisulphate, on an average, 17.4 shoot buds and only 1.6 meristematic nodules were formed from a leaflet. On subculturing, the shoot buds developed into shoots, whereas the meristematic nodules produced three kinds of organized structures that too in varied proportions. Multiplication of shoots was sustained in proliferation medium supplemented with 1.11 µMBAP, 1.43 µMindole-3-acetic acid (IAA) and 135.72 µM adenine hemisulphate. The isolated shoots were rooted and complete plantlets were transferred to potted soil with 100% survival.
Słowa kluczowe
Opis fizyczny
  • Tissue Culture Laboratory, National Botanical Research Institute, Lucknow 226 001, India
  • Tissue Culture Laboratory, National Botanical Research Institute, Lucknow 226 001, India
  • Tissue Culture Laboratory, National Botanical Research Institute, Lucknow 226 001, India
  • Allan EJ (1991) Plant cell culture. In: Stafford A, Warren G (eds) Plant cell and tissue culture. Open University Press, Milton Keynes, pp 1–24
  • Chaturvedi HC, Mitra GC (1975) A shift in morphogenetic pattern in Citrus callus tissue during prolonged culture. Ann Bot (Lond) 39:683–687
  • Chaturvedi HC, Sinha M (1979) Mass clonal propagation of Solanum khasianum through tissue culture. Indian J Exp Biol 17:153–157
  • Chaturvedi HC, Sharma AK, Agha BQ, Jain M, Sharma M (2004a) Production of cloned trees of Populus deltoides through in vitro regeneration of shoots from leaf, stem and root explants and their field cultivation. Indian J Biotechnol 2:203–208
  • Chaturvedi R, Razdan MK, Bhojwani SS (2004b) In vitro morphogenesis in zygotic embryo cultures of neem (Azadirachta indica A. Juss.). Plant Cell Rep 22:801–809. doi:10.1007/s00299-004-0768-0
  • Dai H, Zhang Z, Guo X (2007) Adventitious bud regeneration from leaf and cotyledon explants of Chinese hawthorn (Crataegus pinnatifida Bge. var. major N.E. Br.). In Vitro Cell Dev Biol Plant 43:2–8. doi:10.1007/s11627-006-9008-3
  • D’Onofrio C, Morini S (2002) Increasing NaCl and CaCl₂ concentration in growth medium of quince leaves: I. Effects on somatic embryo and root regeneration. In Vitro Cell Dev Biol Plant 38:366–372. doi:10.1079/IVP2002308
  • D’Onofrio C, Morini S (2003/2004) Simultaneous regeneration of different morphogenic structures from quince leaves as affected by growth regulator combination and treatment length. Biol Plant 47:321–325. doi:10.1023/B:BIOP.0000023872.61646.95
  • D’Onofrio C, Morini S (2005) Development of adventitious shoots from in vitro grown Cydonia oblonga leaves as influenced by different cytokinins and treatment duration. Biol Plant 49:17–21. doi:10.1007/s10535-005-7021-8
  • D’Onofrio C, Morini S, Bellocchi G (1998) Effect of light quality on somatic embryogenesis of quince leaves. Plant Cell Tissue Organ Cult 53:91–98. doi:10.1023/A:1006059615088
  • Eeswara JP, Stuchbury T, Allan EJ, Mordue (Luntz) AJ (1998) A standard procedure for the micropropagation of the neem tree (Azadirachta indica A. Juss.). Plant Cell Rep 17:215–219. doi: 10.1007/s002990050381
  • Feyissa T, Welander M, Negash L (2005) In vitro regeneration of Hagenia abyssinica (Bruce) J. F. Gmel. (Rosaceae) from leaf explants. Plant Cell Rep 24:392–400. doi:10.1007/s00299-005-0949-5
  • George EF (1993) Plant propagation by tissue culture. Part I The technology. Exegetics Ltd., England
  • Johansen DA (1940) Plant microtechnique. McGraw-Hill Book Co. Inc., New York
  • Joshi MS, Thengane SR (1996) In vitro propagation of Azadirachta indica A. Juss. (neem) by shoot proliferation. Indian J Exp Biol 34:480–482
  • Knop W (1865) Quantitative Untersuchungen über den Ernährungsprocess der Pflanze. Landw Versuchs-Stat 7:93–107
  • Misra P, Chaturvedi HC (1991) Influence of inorganic salts on cytokinin induced caulogenesis in leaf segments of Rosmarinus officinalis L. Plant Sci 79:229–235. doi:10.1016/0168-9452(91) 90110-T
  • Mitra GC, Chaturvedi HC (1970) Fruiting plants from in vitro grown leaf tissue of Rauvolfia serpentina Benth. Curr Sci 39:128–129
  • Morini S, D’Onofrio C, Bellocchi G, Fisichella M (2000) Effect of 2, 4-D and light quality on callus production and differentiation from in vitro cultured quince leaves. Plant Cell Tissue Organ Cult 63:47–55. doi:10.1023/A:1006456919590
  • Murashige T (1974) Plant propagation through tissue cultures. Annu Rev Plant Physiol 25:135–166. doi:10.1146/annurev.pp.25.060174.001031
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
  • Murthy BNS, Saxena PK (1998) Somatic embryogenesis and plant regeneration of neem (Azadirachta indica A. Juss.). Plant Cell Rep 17:469–475. doi:10.1007/s002990050427
  • Narayan P, Jaiswal VS (1985) Plantlet regeneration from leaflet callus of Azadirachta indica A. Juss. J Tree Sci 4:65–68
  • Pati PK, Sharma M, Sood A, Ahuja PS (2004) Direct shoot regeneration from leaf explants of Rosa damascena Mill. In Vitro Cell Dev Biol Plant 40:192–195. doi:10.1079/IVP2003503
  • Quraishi A, Koche V, Sharma P, Mishra SK (2004) In vitro clonal propagation of neem (Azadirachta indica). Plant Cell Tissue Organ Cult 78:281–284. doi:10.1023/B:TICU.0000025647. 58548.3d
  • Ramesh K, Padhya MA (1990) In vitro propagation of neem, Azadirachta indica (A. Juss.), from leaf discs. Indian J Exp Biol 28:932–935
  • Salvi ND, Singh H, Tivarekar S, Eapen S (2001) Plant regeneration from different explants of neem. Plant Cell Tissue Organ Cult 65:159–162. doi:10.1023/A:1010672809141
  • Seelye JF, Maddocks DJ, Burge GK, Morgan ER (1994) Shoot regeneration from leaf discs of Limonium perigrinum using thidiazuron. NZ J Crop Hortic Sci Abstr 22:23–29
  • Singh AK, Chand PK, Patnaik S, Chand S (2002) Adventitious shoot organogenesis and plant regeneration from cotyledons of Dalbergia sisoo Roxb., a timber yielding tree legume. Plant Cell Tissue Organ Cult 68:203–209. doi:10.1023/A:1013870803937
  • Thengane SR, Kulkarni DK, Shrikhande VA, Krishnamurthy KV (2001) Effect of thidiazuron on adventitious shoot regeneration from seedling explants of Nothapodytes foetida. In Vitro Cell Dev Biol Plant 37:206–210. doi:10.1007/s11627-001-0036-8
  • Vila SK, Gonzalez AM, Rey HY, Mroginski LA (2003) In vitro plant regeneration of Melia azedarach L.: shoot organogenesis from leaf explants. Biol Plant 47:13–19. doi:10.1023/A:1027364427795
  • Vila SK, Gonzalez AM, Rey HY, Mroginski LA (2005) Plant regeneration, origin, and development of shoot buds from root segments of Melia azedarach L. (Meliaceae) seedlings. In Vitro Cell Dev Biol Plant 41:746–751. doi:10.1079/IVP2005692
  • Welander M (1988) Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. J Plant Physiol 132:738–744
  • Wewetzer A (1998) Callus cultures of Azadirachta indica and their potential for the production of azadirachtin. Phytoparasitica 26:47–52
  • Wewetzer A, Schulz FA (1994) Plantlet regeneration from tissue culture of Azadirachta indica (Juss.) In: Proceedings of 8th International Congress on Plant Tissue and Cell Culture p 12. Florence
  • Yepes LM, Aldwinckle HS (1994) Factors that affect leaf regeneration efficiency in apple, and effect of antibiotics in morphogenesis. Plant Cell Tissue Organ Cult 37:257–269
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.