Interaction between voltage-dependent sodium channel and its site-3 ligand modified by exposure to 50Hz electromagnetic field

• Autorzy: Jankowska M. , Pawlowska-Mainville A. , Stankiewicz M. , Rogalska J. , Wyszkowska J.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Extremely low-frequency (50 Hz) electromagnetic field (ELF-EMF) is produced by electrical appliances and electric power transmission lines. It affects organism functions and induces among others: the discrete changes in neuronal membrane potential, the increase of calcium channel activity and intracellular concentration of Ca2+. In insects, ELF-EMF causes an increase in a level of stress hormone – the octopamine. Site-3 ligands of voltage-dependent sodium channel inhibit fast inactivation of the channel. Receptor site-3 is located in the immediate vicinity of voltage sensor of the sodium channel. The LqhαIT toxin from the scorpion Leiurus quinquestriatus venom is an example of a site-3 ligand. The aim of our study was to investigate whether ELF-EMF exposure affects the action of the LqhαIT toxin on an axonal membrane. METHODS: We have observed an action of 0.7 mT ELF-EMF and LqhaIT (5×10-8 M) on the bioelectrical activity of an isolated nerve cord of the cockroach – Periplaneta americana. The tests have been conducted using extracellular electrodes, which allowed to record the total bioelectric activity from one connective and one cercal nerve. Additionally, we have performed in-vivo tests, during which we have estimated the impact of 0.7 mT and 7 mT ELF-EMF on the degree of the insect paralysis caused by the LqhαIT (10-8 M and 10-7 M). RESULTS: ELF-EMF exposure induced important changes in the LqhαIT activity – much higher during synaptic transmission than on a level of “cable transmission”. In-vivo, electromagnetic field probably accelerates the insect detoxification process because paralysis induced by the toxin was much lower after the exposure than in control. CONCLUSION: Results of our experiments can suggest the changes in organism sensitivity to neurotoxic ligands.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S105-S106

Twórcy

autor

Jankowska M.

• Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland

autor

Pawlowska-Mainville A.

• Department of First Nations Studies, University of Northern British Columbia, Prince George, Canada

autor

Stankiewicz M.

• Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland

autor

Rogalska J.

• Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland

autor

Wyszkowska J.

• Faculty of Biology and Environmental Protection, Nicolaus Copernicus University, Torun, Poland