EN
The aim of the study was to describe polymorphism of BoLA-DRB3.2 in Polish Holstein-Friesians. Alleles were identified according to PCR-RFLP procedure using a modification to the van Eijk’s proposal [1992]. Restriction patterns were obtained with BstYI, HaeIII and RsaI enzymes. The study presented variability of all the alleles estimated in two herds. 752 animals were genotyped and informative according to previously described RFLP patterns. The most frequent homozygous animals were DRB3.2*24. The frequency was 0.066 and 0.078 in the sampled herds. No DRB3.2*03 nor DRB3.2*28 homozygous animals were found in one herd as opposite to another herd where homozygous animals DRB3.2*03 occurred with frequency 0.26 and animals DRB3.2*28 occurred with frequency 0.59. Homozygous DRB3.2*gba was not observed in that herd. The differences in allele frequencies may be associated with herd production and fitness status.