Evaluation of two prototype directional freezing methods and a 2ml flattened straw for cryopreservation of boar semen

• Autorzy: Puglisi R. , Bornaghi V. , Severgnini A. , Vanni R. , Montedoro M. , Galli A.
• Języki publikacji: EN

Abstrakty

EN

Based on previous assessments on stallions, 40 ejaculates of 20 Duroc boars were split and evenly frozen with a conventional vapour freezing method and two directional, drum and directional, prototype methods using commercial extenders and relative standard procedures. The directional prototype was provided with a double internal setup that allowed the positioning of experimental 2ml flat straws with 1 billion sperm (Flat) in a fixed support, or both classical 0.5 ml paillettes with 250 million spermatozoa and flats in a rotating drum designed so as to ensure a more uniform heat exchange. Preliminary tests for individuation of the most appropriate thawing rate showed beneficial effects (P≤0.05) of thawing the sperm at 50°C for 13 s when compared to 42°C for 20 s, in terms of total motility (42.8±8.4% and 35.6±6.8%, respectively). With regard to freezing/packaging methods, major improvements (P≤0.05) were shown for the drum method with paillettes for total motility (38.6±14.2%) assessed immediately after thawing, when compared with the conventional (29.4±13.3%) and the directional methods with flats (30.2±12.8%), and for total motility (P≤0.01) assessed following incubation for 120 min at 37°C after thawing (24.8±11.6%) with respect to the conventional method (15.6±10.9%). Despite the statistical non-significance of results, both the prototype freezing approaches using the experimental flat straw showed some improvements in functional parameters assessed by cytofluorometry when compared to the conventional method.

Słowa kluczowe

EN

prototype; freezing method; sperm quality; cryopreservation; boar semen; animal breeding

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2017
• Tom: 35
• Numer: 4
• Opis fizyczny: p.397-405,ref.

Twórcy

autor

Puglisi R.

• Istituto Sperimentale Italiano Lazzaro Spallanzani, Loc. La Quercia, 26027, Rivolta d’Adda (CR), Italy

autor

Bornaghi V.

• Istituto Sperimentale Italiano Lazzaro Spallanzani, Loc. La Quercia, 26027, Rivolta d’Adda (CR), Italy

autor

Severgnini A.

• Istituto Sperimentale Italiano Lazzaro Spallanzani, Loc. La Quercia, 26027, Rivolta d’Adda (CR), Italy

autor

Vanni R.

• Istituto Sperimentale Italiano Lazzaro Spallanzani, Loc. La Quercia, 26027, Rivolta d’Adda (CR), Italy

autor

Montedoro M.

• Istituto Sperimentale Italiano Lazzaro Spallanzani, Loc. La Quercia, 26027, Rivolta d’Adda (CR), Italy

autor

Galli A.

• CREA - Research Centre for Animal Production and Aquaculture, 26900, Lodi, Italy

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