Effects of solar ultraviolet radiation on photochemical efficiency of Chaetoceros curvisetus (Bacillariophyceae)

• Autorzy: Guan W.C. , Li P. , Jian J. B. , Wang J. Y. , Lu S. H.
• Języki publikacji: EN

Abstrakty

EN

To assess the short- and long-term impacts of Ultraviolet radiation (UVR, 280–400 nm) on the red tide alga Chaetoceros curvisetus, we exposed cells to three different solar radiation treatments–PAB:280–700 nm, PA:320–700 nm, and P:400–700 nm under 20°C incubated temperature. Short-term exposures were investigated: the photochemical efficiency (ΦPSII) versus irradiance curves under six levels of solar radiation by covering the incubators with a variable number of neutral density screens (the irradiance thus varied from 100 to 3%) lasting 1 h, and longterm exposures were designed to assess how the cells acclimate to solar radiation (the growth, UVabc and ratio of repair to damage rates of D1 protein were detected). A significant decrease in the photochemical efficiency (ΦPSII) at high irradiance (100% of incident solar radiation, 261.6 Wm⁻²) was observed in short-term exposure (1 h). UVR-induced photoinhibition was reduced to 7% in 3% solar radiation (4.08 Wm⁻²), compared with 66% in 100% solar radiation (261.6 Wm⁻²). In long-term experiments (11 days) using batch cultures, cell densities during the first 6 days were relatively constant for treatment P, and decreased slightly under PA and PAB treaments, reflecting a change in the irradiance experienced in the laboratory to that of incident solar irradiance. Thereafter, cell density increased and UV-induced photoinhibition decreased with the following days, indicating acclimation to solar UV. At the end of experiment, cells were found to exhibit both higher ratios of repair to UV-related damage and increased concentrations of UV-absorbing compounds, whose maximum absorption was found to be at 329 nm. Our data indicate that C. curvisetus is sensitive to ultraviolet radiation, but was able to acclimate relatively rapidly (ca. 6 days) by synthesizing UV-absorbing compounds and by increasing the rates of repair processes of D1 protein in PSII.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 3
• Opis fizyczny: p.979-986,fig.,ref.

Twórcy

autor

Guan W.C.

• School of Life Sciences, Wenzhou Medical College, Wenzhou 325035, Zhejiang, China
• College of Life Science South China Normal University, Guanzhou 510631, Guangdong, China

autor

Li P.

• Marine Biology Institute, Shantou University, Shantou 515063, Guangdong, China

autor

Jian J. B.

• Marine Biology Institute, Shantou University, Shantou 515063, Guangdong, China

autor

Wang J. Y.

• College of Life Science South China Normal University, Guanzhou 510631, Guangdong, China

autor

Lu S. H.

• Research Center for Harmful Algae and Aquatic Environment, Jinan University, Guangzhou 510006, China
• College of Life Science South China Normall University, Guanzhou 510631, Guangdong, China

Bibliografia

• Barber J (1995) Molecular basis of the vulnerability of photosystem II to damage by light. Aust J Plant Physiol 22:201–208
• Barbieri ES, Villafañe VE, Helbling EW (2002) Experimental assessment of UV effects on temperate marine phytoplankton when exposed to variable radiation regimes. Limnol Oceanogr 47:1648–1655
• Beardall J, Raven JA (2004) The potential effects of global climate change on microalgal photosynthesis, growth and ecology. Phycologia 43:26–40
• Behrenfeld MJ, Hardy JT, Lee H II (1995) Ultraviolet-B radiation effects on inorganic nitrogen uptake by natural assemblages of oceanic phytoplankton. J Phycol 31:25–36
• Boelen P, De-Boer MK, Kraay GW, Veldhuis MJW, Buma AGJ (2000) UVBR-induced DNA damage in natural marine picoplankton assemblages in the tropical Atlantic Ocean. Mar Ecol Prog Ser 193:1–9
• Demming-Adams B, Adams WW (1992) Photoprotection and other responses of plants to high light stress. Ann Rev Plant Physiol 43:599–626
• Franklin LA, Forster RM (1997) The changing irradiance environment: consequences for marine macrophyte physiology, productivity and ecology. Eur J Phycol 32:207–232
• Gao KS, Guan WC, Helbling EW (2007a) Effects of solar ultraviolet radiation on photosynthesis of the marine red tide alga Heterosigma akashiwo (Raphidophyceae). J Photochem Photobiol B Biol 86(2):140–148
• Gao KS, Wu YP, Li G, Wu HY, Villafañe VE, Helbling EW (2007b) Solar UV radiation drives CO₂ fixation in marine phytoplankton: a double-edged sword. Plant Physiol 144:54–59
• Garcia-Pichel F (1994) A model for internal self-shading in planktonic organisms and its implications for the usefulness of ultraviolet sunscreens. Limnol Oceanogr 39:1704–1717
• Genty BE, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92
• Greenberg BM, Gaba V, Canaani O, Malkin S, Mattoo AK, Edelman M (1989) Separate photosensitizers mediate degradation of the 32-kDa Photosystem II reaction centre protein in visible and UV spectral regions. Proc Natl Acad Sci USA 86:6617–6620
• Guan WC, Gao KS (2008) Light histories influence the impacts of solar ultraviolet radiation on photosynthesis and growth in a marine diatom, Skeletonema costatum. J Photochem Photobiol B Biol 91(2–3):151–156
• Guan WC, Gao KS (2010a) Enhanced calcification ameliorates the negative effects of UV radiation on photosynthesis in the calcifying phytoplankter Emiliania huxleyi. Chin Sci Bull 55(7):588–593
• Guan WC, Gao KS (2010b) Impacts of UV radiation on photosynthesis and growth of the coccolithophore Emiliania huxleyi (Haptophyceae). Environ Exp Bot 67:502–508
• Guan WC, Lu SH (2010) The short and long-term response of Scripsiella trochoidea (Pyrrophyta) to solar ultraviolet radiation. Photosynthetica 48(2):287–293
• Guillard RRL, Ryther JH (1962) Studies on marine planktonic diatoms. I. Cyclotella nana (Hustedt) and Detonula confervaceae (Cleve). Can J Microbiol 8:229–239
• Häder DP, Lebert M, Marangoni R, Colombetti G (1999) ELDONET–European light dosimeter network hardware and software. J Photochem Photobiol B Biol 52(1–3):51–58
• Häder DP, Kumar HD, Smith RC, Worrest RC (2007) Effect of solar UV radiation on aquatic ecosystems and interactions with climate change. Photochem Photobiol 6:267–285
• Halac SR, Villafañe VE, Helbling EW (2010) Temperature benefits the photosynthetic performance of the diatoms Chaetoceros gracilis and Thalassiosira weissflogii when exposed to UVR. J Photochem Photobiol B Biol. doi:10.1016/j.jphotobiol.2010. 07.003
• Helbling EW, Chalker BE, Dunlap WC, Holm-Hansenn O, Villafañe VE (1996) Photoacclimation of Antarctic marine diatoms to solar ultraviolet radiation. J Exp Mar Biol Ecol 204:85–101
• Helbling EW, Gao KS, Goncalves RJ, Wu HY, Villafañe VE (2003) Utilization of solar UV radiation by coastal phytoplankton assemblages off SE China when exposed to fast mixing. Mar Ecol Prog Ser 259:59–66
• Heraud P, Beardall J (2000) Changes in chlorophyll fluorescence during exposure of Dunaliella tertiolecta to UV radiation indicate a dynamic interaction between damage and repair processes. Photosynth Res 63:123–134
• Karentz D, Clearver JE, Mitchell DL (1991) Cell survival characteristics and molecular responses of Antarctic phytoplankton to ultraviolet-B radiation. J Phycol 27:326–341
• Lesser MP, Cullen JJ, Neale PJ (1994) Carbon uptake in a marine diatom during acute exposure to ultraviolet B radiation: Relative importance of damage and repair. J Phycol 30:183–192
• Liang Y, Beardall J, Heraud P (2006) Effect of UV radiation on growth, chlorophyll fluorescence and fatty acid composition of Phaeodactylum tricornutum and Chaetoceros muelleri (Bacillariophyceae). Phycologia 45(6):605–615
• Marwood CA, Smith REH, Furgal JA, Charlton MN, Solomon KR, Greenberg BM (2000) Photoinhibition of natural phytoplankton assemblages in Lake Erie exposed to solar ultraviolet radiation. Can J Fish Aquat Sci 57:371–379
• Máté Z, Sass L, Szekeres M, Vass I, Nagy F (1998) UV-B induced differential transcription of psbA genes endoding the D1 protein of Photosystem II in the cyanobacterium Synechocystis 6803. J Biol Chem 273:17439–17444
• Mengelt C, Prézelin BB (2005) UVA enhancement of carbon fixation and resilience to UV inhibition in the genus Pseudo-nitzschia may provide a competitive advantage in high UV surface waters. Mar Ecol Prog Ser 301:81–93
• Neori A, Vernet M, Holm-Hansen O, Haxo FT (1988) Comparison of chlorophyll far-red and red fluorescence excitation spectra with photosynthetic oxygen action spectra for photosystem II in algae. Mar Ecol Prog Ser 44:297–302
• Porra RJ (2002) The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynth Res 73:149–156
• Renger G, Völker M, Eckert H, Fromme R, Hohm-Veit S, Gräber P (1989) On the mechanism of photosystem II deterioration by UV-B irradiation. Photochem Photobiol 49:97–105
• Sinha RP, Klisch M, Gröniger A, Häder DP (1998) Ultravioletabsorbing/ screeningsubstances in cyanobacteria, phytoplankton and macroalgae. J Photochem Photobiol B Biol 47:83–94
• Sommaruga R, Garcia-Pichel F (1999) UV-absorbing mycosporinelike compounds in planktonic and benthic organisms from a high-mountain lake. Arch Hydrobiol 144(3):255–269
• Wang JH, Wu JYO (2009) ccurrence and potential risks of harmful algal blooms in the East China Sea. Sci Total Environ 407:4012–4021
• Weatherhead EC, Andersen SB (2006) The search of the signs of recovery of ozone layer. Nature 441:39–45
• Wu HY, Gao KS, Villafañe VE, Watanabe T, Helbling EW (2005) Effects of solar UV radiation on morphology and photosynthesis of filamentous cyanobacterium Arthrospira platensis. Appl Environ Microbiol 71:5004–5013
• Wu HY, Gao KS, Wu HY (2009) Responses of a marine red tide alga Skeletonema costatum (Bacillariophyceae) to long-term UV radiation exposures. J Photochem Photobiol B Biol 94(2):82–86
• Xiong FS (2001) Evidence that UV-B tolerance of the photosynthetic apparatus in microalgae is related to the D1-turnover mediated repair cycle in vivo. J Plant Physiol 158:285–294
• Zheng YQ, Gao KS (2009) Impacts of solar UV radiation on the photosynthesis, growth, and UV-absorbing compounds in Gracilaria lemaneiformis (Rhodophyta) grown at different nitrate concentrations. J Phycol 45:314–323

Uwagi

rekord w opracowaniu