Evaluating environmental pollution by applying oxidative stress biomarkers as bioindicators of water pollution in fish

• Autorzy: Valon M. , Valbona A. , Fahri G. , Qenan M. , Dhurat K. , Fatmir C.
• Języki publikacji: EN

Abstrakty

EN

Antioxidant enzyme activities of fish (Cyprinus carpio L.) were determined in order to detect the effects of pollution of the Sitnica River in Kosovo on fish physiology and biochemistry. Activities of three antioxidant defense enzymes as catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST) in the blood of fish were chosen as bioindicators. Fish (n=21) were sampled during March-May and AugustSeptember 2010-12 from Ferizaj, Vragoli, and Plemetin (Sitnica River). Plasmatic CAT, SOD, and GST were significantly higher (P<0.001) in fish from polluted Vragoli and Plementin sites than Ferizaj. Ferizaj is a source of the river site, so is less contaminated than the two other sites. These three enzymes constitute a sensitive biochemical bioindicator and can be used for detection of chemical pollution in fish. Based on results of our research we can conclude that the Sitnica River is polluted mainly by industrial and urban discharge of liquid waste products. As a consequence, legal actions need to be taken in order to prevent environmental pollution on the site.

Słowa kluczowe

EN

environment pollution; oxidative stress; biomarker; bioindicator; water pollution; fish; Cyprinus carpio; catalase; superoxide dismutase; Sitnica River; antioxidative enzyme; glutathione S-transferase

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2013
• Tom: 22
• Numer: 5
• Opis fizyczny: p.1519-1523,fig.,ref.

Twórcy

autor

Valon M.

• Faculty of Geosciences and Technology, University of Prishtina, Mitrovice, Kosovo

autor

Valbona A.

• Department of Biology, Faculty of Natural Sciences, Tirana University, Tirana, Albania

autor

Fahri G.

• University of Medical Sciences “Rezonance”, Prishtina, Kosovo

autor

Qenan M.

• Environmental Protection Agency (KEPA)

autor

Dhurat K.

• Department of Biology, Faculty of Natural Sciences, University of Prishtina, Prishtine, Kosovo

autor

Fatmir C.

• University of Medical Sciences “Rezonance”, Prishtina, Kosovo

Bibliografia

• 1. SHARBIDRE A. A., GOHIL D. R., GADEKAR S. B. Multibiomarker approach in Oreochromis niloticus (Peters, 1892) fish to assess the impact of pollution in Mula River. The Journal of ISRJ. ISSN 2230-7850 Vol II, ISSUE IV, May 2012.
• 2. VELKOVA-JORDANOSKA L., KOSTOSKI L.G., JORDANOSKA B. Antioxidant enzymes in fish as biochemical indicators of aquatic pollution. Bulgarian Journal of Agricultural Sciences, 14, (2), 235, 2008.
• 3. MATZINGER A., SCHMID M., VELJANOSKA-SARAFILOSKA E., PATCEVA S., GUSESKA D., MULLER B., STURM M., WUEST A. Eutrophication of ancient Lake Ohrid: Global warming amplifies detrimental effect of increased nutrient inputs. Limnol. Oceanogr. 52, (1), 338, 2007.
• 4. VINODHINI R., NARAYANAN M. The impact of toxic heavy metals on the hematological parameters in common carp (Cyprinus carpio L.): Iran. J. Environ. Health. Sci. Eng., V. 6, (1), 23, 2009.
• 5. ZIKIC R. V., STAJN A. S., PAVLOVIC S. Z., OGNANOVIC B. I., SAICIC Z. S. Activities of Superoxide Dismutase and Catalase in Erithrocytes and Plasma Transaminases of Goldfish (Carassius auratus gibelio Bloch.) Exposed to Cadmium. Physiol. Res., 50, 105, 2001.
• 6. BILINSKI F., GASHI F., BILINSKI H. Establishing of Monitoring Network on Kosovo Rivers (Drini i Bardhë, Morava e Binçës, Lepenc and Sitnica. BALWOIS 2010 - Ohrid, Republic of Macedonia - 25, 29 May 2010.
• 7. DIMITROVA M.S.T., TSINOVA V., VELCHEVA V. Combined effects of zinc and lead on the hepatic superoxide dismutase-catalase system in carp (Cyprinus carpio). Comp. Biochem. Phys. C., 108, 43, 1994.
• 8. BERISHA L., ARBNESHI T., RUGOVA M. The level concentration of lead, cadmium, copper, zinc and phenols in the water of Sitnica River. J. Int BALWOIS 2008 – Ohrid, Republic of Macedonia. 2008.
• 9. MALINS D.C., HAIMANOT R. The etiology of cancer: hydroxyl radical-induced DNA lesions in histological normal livers of fish from a population with liver tumors. Aquat. Toxicol. 20, 123, 1991.
• 10. SIMA A. R., MORAVEC F., GUEMEZ R. J. Pathogenic effects of the eggs of Capillaria cichlasomae (Nematoda: Capillariidea) in the liver of the cichlid Cichlasoma urophthalmus (Gunther) in Mexico. J. Fish Dis., 19, 175, 1996.
• 11. VELKOVA-JORDANOSKA L. Histopatological and molecular-diagnostical study of the hepatic capillarity in black barbel (Barbus meridionalis petenyi Heck.) in Lake Ohrid. MSc Thesis, Univ. St.Kiril and Methodius, Skopje, R. Macedonia (Mc). 2002.
• 12. VELKOVA-JORDANOSKA L. Histopatological analysis of the biliary tree in black barbel (Barbus meridionalis petenyi Heckel; Pisces) in Lake Ohrid. SI Hydrobiological Institute-Ohrid, Review, 35, (1), 119, (Mc). 2003.
• 13. KUTHAN H., HAUSSMANN H. J., WERRINGLOVER J. A spectrophotometric assay for superoxide dismutase activities in crude tissue fractions. Biochem. J., 237, 175, 1986.
• 14. MEDJA N., PANARITI E., STRIKNIQI A. Influence of cyanotoxins in histology of Carassius carassius L. Natura Montenegrina, 9, (3), 615, 2011.
• 15. DING W.X., SHEN H.M., ONG C.N. Microcystin cyanobacteria extract induces cytoskeleton disruption and intracellular glutathione alteration in hepatocytes. Environ. Health Persp. 108, 605, 2000.
• 16. JASTRZEBSKA E.W., KAWCZUGA D. Antioxidant status and lipid peroxidation in blood of common carp (Cyprinus carpio L.). Pol. J. Environ. Stud. 20, (3), 541, 2011.
• 17. McCORD, J. M., FRIDOVICH I. Superoxide dismutase: an enzymatic function for erytrocuprein (Hemocuprein). J. Biol. Chem., 244, (22), 6049, 1969.
• 18. LIN C. T., LEE T. L., DUAN K. J., SU J. C. Purification and Characterization of Black Porgy Muscle Cu/Zn Superoxide Dismutase. Zool. Stud., 40, (2). 84, 2001.
• 19. DI GULIO R.T., HABIG C., GALLAGHER E.P. Effects of black rock harbor sediments on indices of biotransformation, oxidative stress and DNA integrity in channel catfish. Aquat. Toxicol. 26, 1, 1993.
• 20. LI X., LIU Y., SONG L., LIU J. Responses of antioxidant systems in the hepatocytes of common carp (Cyprinus carpio L.) to the toxicity of microcystin-LR. Toxicon. 42, 85, 2003.
• 21. YILDIRIM N. C., BENZER F., DANABAS D. Evaluation of environmental pollution at Munzur River of tunceli applying oxidative stress biomarkers in Capoeta trutta (Heckel, 1843). The Journal of Animal & Plant Sciences, 21, (1), 66, 2011.
• 22. ROGANOVIC-ZAFIROVA D., JORDANOVA M., PANOV S. VELKOVA-JORDANOSKA L. Hepatic capillaries in the Mediterranean barbel (Barbus meridionalis petenyi Heck.) from Lake Ohrid. Folia Veterinaria, 47, (1), 35, 2003.
• 23. http://www.kosovoguide.com 2012 (Accessed 3th October 2012).
• 24. FIHLO D.W. Fish antioxidant defenses-a comparative approach. Braz. J. Med. Biol. Res. 29, 1735, 1996.
• 25. MCCORD J. M., FRIDOVICH I. The Utility of Superoxide Dismutase in Studying Free Radical Reactions: II. The mechanism of the mediation of Cytochrome c reduction by a variety of electron carriers. J. Biol. Chem., 245, (6), 1374, 1970.
• 26. ISANI G., SARLI G., ANDREANI G., BRUNETTI B., MARROCCO R., CARPENÉ E., BEHA G., MORANDI F.,BENAZZI C. Effects of waterborne copper on gills catalase and blood biochemistry in Gilthead Sea bream (Sparus aurata L.). J. Elem. 17, (2), 255, 2012.