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2015 | 84 | 3 |
Tytuł artykułu

A simple way to overcome the recalcitrance of the water fern Ceratopteris thalictroides (L.) Brongn. to cryopreservation

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Ceratopteris thalictroides is a water fern very sensitive to both dehydration and low temperature. This study focuses on the cryopreservation of this species by encapsulation-dehydration technique, in particular on the effects of pre-culture step, alginate bead size and the physical conditions of culture on the cryopreservation efficiency. Encapsulated and non-pre-cultured gametophytes did not survive cooling with liquid nitrogen. When cryopreservation was preceded by a 2-week period of pre-culture, regrowth reached 42.1%. Reduction in the size of the alginate bead, and culture in total darkness resulted in improved gametophyte regrowth capacity (75.5% or 81.7%, respectively). The best results (91.3%) were obtained when all factors tested occurred simultaneously. The gametophytes recovered very quickly and sporophytes were formed within 4 weeks after rewarming. These simple improvements can be used, not only for the cryopreservation of gametophytes in cryptogams but also for some recalcitrant species of seed plants.
Wydawca
-
Rocznik
Tom
84
Numer
3
Opis fizyczny
p.385-388,fig.,ref.
Twórcy
autor
  • Faculty of Plant Experimental Biology, Polish Academy of Sciences, Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973 Warsaw, Poland
  • Faculty of Plant Experimental Biology, Polish Academy of Sciences, Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973 Warsaw, Poland
autor
  • Faculty of Plant Experimental Biology, Polish Academy of Sciences, Botanical Garden - Center for Biological Diversity Conservation in Powsin, Prawdziwka 2, 02-973 Warsaw, Poland
Bibliografia
  • 1.Cruz-Cruz CA, González-Arnao MT, Engelmann F. Biotechnology and conservation of plant biodiversity. Resources. 2013;2:73-95. http:// dx.doi.org/10.3390/resources2020073
  • 2.Benelli C, de Carlo A, Engelmann F. Recent advances in the cryo-preservation of shoot-derived germplasm of economically important fruit trees of Actinidia, Diospyros, Malus, Olea, Prunus, Pyrus and Vitis. Biotechnol Adv. 2013;31:175-185. http://dx.doi.org/10.1016/). biotechadv.2012.09.004
  • 3.Panis B, Piette B, Swennen R. Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Sci. 2005;168:45-55. http://dx.doi.org/10.1016/j.plantsci.2004.07.022
  • 4.Bui TVL, Ross IL, Jakob G, Hankamer B. Impact of procedural steps and cryopreservation agents in the cryopreservation of chlorophyte microalgae. PloS ONE. 2013;8(11):e78668. http://dx.doi.org/10.1371/ journal.pone.0078668
  • 5.Mikuła A, Makowski D, Walters C, Rybczyński JJ. Exploration of cryo-methods to preserve tree and herbaceous fern gametophytes. In: Fernández H, Kumar A, Revilla MA, editors. Working with ferns: issues and applications. New York, NY: Springer Science; 2011. p. 173-192. http://dx.doi.org/10.1007/978-1-4419-7162-3_13
  • 6.Ogawa Y, Sakurai N, Oikawa A, Kai K, Morishita Y, Mori K, et al. High-throughput cryopreservation of plant cell cultures for functional genomics. Plant Cell Physiol. 2012;53:943-952. http://dx.doi. org/10.1093/pcp/pcs038
  • 7.Sakai A, Engelmann F. Vitrification, encapsulation-vitrification and droplet-vitrification: a review. CryoLetters. 2007;28:151-172.
  • 8.Barnicoat H, Cripps R, Kendon J, Sarasan V. Conservation in vitro of rare and threatened ferns - case studies of biodiversity hotspot and island species. In Vitro Cell Dev Biol Plant. 2011;47:37-45. http:// dx.doi.org/10.1007/s11627-010-9303-x
  • 9.Mikuła A, Jata K, Rybczyński JJ. Cryopreservation strategies for Cyathea australis (R. Br.) Domin. CryoLetters. 2009;30:429-439.
  • 10.Pence VC. Cryopreservation of in vitro grown fern gametophytes. Am Fern J. 2000;90:16-23. http://dx.doi.org/10.2307/1547258
  • 11.Rodpradit S, Thavipoke P, Thammasiri K. Cryopreservation of young Platycerium ridleyi H. Christ. sporophytes by encapsulation/vitrification technique. In: Proceedings of the 29th Congress on Science and Technology of Thailand; 2003 Sep 20-22; Khon Kean, Thailand. Bangkok: Mahidol University; 2003. p. 71-73.
  • 12.Masuyama S. Cryptic species in the fern Ceratopteris thalictroides (Parkeriaceae). III. Referential diagnostic characters of three cryptic species. J Plant Res. 2008;121:279-286. http://dx.doi.org/10.1007/ s10265-008-0159-7
  • 13.Murashige T, Skoog F. A revised medium for rapid growth and bioas-says with tobacco tissue cultures. Physiol Plant. 1962;15:473-497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
  • 14.Fabre J, Dereuddre J. Encapsulation-dehydration: a new approach to cryopreservation of Solanum shoot tips. CryoLetters. 1990;11:413-426.
  • 15.Roach T, Krieger-Liszkay A. The role of the PsbS protein in the protection of photosystems I and II against high light in Arabidopsis thaliana. Biochim Biophys Acta. 2012;1817:2158-2165. http://dx.doi. org/10.1016/j.bbabio.2012.09.011
  • 16.Mazur P, Seki S. Survival of mouse oocytes after being cooled in a vitrification solution to -196°C at 95° to 70 000°C/min and warmed at 610° to 118 000°C/min: a new paradigm for cryopreservation by vitrification. Cryobiology. 2011;62:1-7. http://dx.doi.org/10.1016/). cryobiol.2010.10.159
  • 17.Hickok LG, Warne TR, Fribourg RS. The biology of the Ceratopteris and its use as a model system. Int J Plant Sci. 1995;156:332-345. http:// dx.doi.org/10.1086/297255
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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