Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

• Autorzy: Agnihotri R.K. , Mishra J. , Nandi S. K.
• Języki publikacji: EN

Abstrakty

EN

An efficient in vitro regeneration protocol and field performance of a multipurpose bamboo species Dendrocalamus hamiltonii Nees et Arn. Ex Munro has been demonstrated using single node cuttings taken from the lateral branches of a 20-year-old bush. Axillary buds on the nodal explant sprouted within 10 days of culture on Murashige and Skoog (MS) medium without any plant growth substance. High-frequency proliferation was induced on the propagules (small clusters with 3–5 multiple shoots and rhizomatous portions). Subsequent removal of the shoots (about 1.5 cm) from the rhizomatous portion of propagules (shoot cut) influenced the plantlet formation capacity. A multiplication of about 20-folds was achieved on MS medium supplemented with 8 µM BAP and 1 µM NAA. Rooting efficiency was also markedly enhanced (>90%) when the propagules, following shoot cut, were placed on to MS medium supplemented with 100 µM IBA for 10 days and then transferred to IBA-free medium. This is the first report from this species where 20-fold increment in multiplication was observed at the end of second subculture followed by >90% rooting. The hardened plants, established in the field, exhibited normal growth; their physiological performance has been monitored at 6-month intervals. The rate of photosynthesis increased from 3.55 µmol CO₂ m⁻² s⁻¹ (hardened, ready for field transfer) to 5.44 µmol m⁻² s⁻¹ (6 months of field transfer); following a year of plantation net photosynthesis recorded was 14.0 µmol CO₂ m⁻² s⁻¹ while after 1.5 years it was 12.76 µmol CO₂ m⁻² s⁻¹. These values were compared with those observed for the mother bush. Genetic fidelity of these regenerants was established by RAPD analysis advocating clonal propagation of this species through nodal segment culture and its commercial cultivation.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 5
• Opis fizyczny: p.961-967,fig.,ref.

Twórcy

autor

Agnihotri R.K.

• Biotechnological Applications Theme, G.B. Plant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, Uttarakhand, India
• Department of Botany, School of Life Sciences, Dr B.R. Ambedkar University, Agra 282 002, Uttar Pradesh, India

autor

Mishra J.

• Biotechnological Applications Theme, G.B. Plant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, Uttarakhand, India

autor

Nandi S. K.

• Biotechnological Applications Theme, G.B. Plant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, Uttarakhand, India

Bibliografia

• Bag N (2001) Mass propagation of tea, maggar bamboo and dev ringal. D Phil Thesis, HNB Garhwal University, Srinagar, India, 168 p
• Chambers SM, Heuch JHR, Pirre C (1991) Micropropagation and in vitro flowering of the bamboo Dendrocalamus hamiltonii Munro. Plant Cell Tissue Organ Cult 27:45–48. doi:10.1007/ BF00048205
• Das M, Pal A (2005) Clonal propagation and production of genetically uniform regenerants from axillary meristem of adult bamboo. J Plant Biochem Biotechnol 14:185–188
• Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bull 19:11–15
• Gielis J, Peeters H, Gillis K, Oprimes J, Debergh PC (2002) Tissue culture strategies for genetic improvement of bamboo. Acta Hortic 552:195–203
• Gimenez C, Garcia ED, Enrech NXD, Blanca I (2001) Somaclonal variation in banana: cytogenetic and molecular characterization of somaclonal variant CIEN BTA-03. Vitro Cell Dev Biol Plant 37:217–222
• Godbole S, Sood A, Thakur R, Sharma M, Ahuja PS (2002) Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo, Dendrocalamus hamiltonii Nees et Arn Ex Munro. Curr Sci 83:885–889
• Isabel NL, Tremblay MM, Tremblay FM, Bousquet J (1993) RAPD as an aid to evaluate the genetic integrity of somatic embryogenesis derived population of Picea mariana (Mill). Theor Appl Genet 86:81–87. doi:10.1007/BF00223811
• Kumar A, Nandi SK, Bag N, Palni LMS (2002) Tissue culture studies in two important orchid taxa: Rhynchostylis retusa (L) Bl. and Cymbidium elegans Lindl. In: Nandi SK, Palni LMS, Kumar A (eds) Role of plant tissue culture in biodiversity conservation and economic development. Gyanodaya Prakashan, Nainital, pp 113–124
• Kumar A, Palni LMS, Nandi SK (2003) The effect of light and gelling agent on micropropagation of Rosa damascena Mill. And Rhynchostylis retusa (L.) Bl. J Hortic Sci Biotechnol 78(6): 786–792
• Millan T, Osuna F, Cobos S, Torres AM, Cubero JJ (1996) Using RAPDs to study phylogenetic relationships in Rosa. Theor Appl Genet 92:273–277. doi:10.1007/BF00223385
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
• Nayak S, Rout GR, Das P (2003) Evaluation of the genetic variability in bamboo using RAPD markers. Plant Soil Environ 49(1):24–28
• Negi SS, Pal RN, Enrich C (1980) Tree fodders in Himachal Pradesh. German Agency for Technical Cooperation (GTZ), Eschborn (FRG), India
• Prutpongse P, Gavinlertvatana P (1992) In vitro micropropagation of 54 species from 15 genera of bamboo. Hortic Sci 27:453–454
• Ramanayake SMSD, Yakanadawala K (1997) Micropropagation of the giant bamboo (Dendrocalamus giganteus Munro) from nodal explants of field grown culms. Plant Sci 129:213–223. doi: 10.1016/S0168-9452(97)00185-4
• Rani V, Parida A, Raina SN (1995) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh. Plant Cell Rep 14:459–462. doi:10.1007/BF00234055
• Rani V, Singh KP, Shiran M, Goel S, Devarumath RM, Sreenath HL, Raina SN (2000) Evidence for new nuclear and mitochondrial genome organizations among high frequency somatic embryogenesis-derived plants of allotetraploid Coffea arabica L. (Rubiaceae). Plant Cell Rep 19:1013–1020. doi:10.1007/ s002990000228
• Rao IVR, Yusoff AM, Rao AN, Shastry CB (1990) Propagation of bamboo and rattan through tissue culture. The IDRC Bamboo and Ratan Research Network, Canada, pp 1–60
• Rival A, Bertrand L, Beale T, Combes M-C, Trouslot P, Leshermes P (1998) Suitability of RAPD analysis for detection of somaclonal variation in oil palm (Elaeis guineensis Jacq.). Plant Breed 117:73–76. doi:10.1111/j.1439-0523.1998.tb01451.x
• Saxena S, Dhawan B (1999) Regeneration and large-scale propagation of bamboo (Dendrocalamus strictus Nees) through somatic embryogenesis. Plant Cell Rep 18:438–443. doi:10.1007/ s002990050600
• Snedecor GW, Cochran WG (1967) Statistical methods. Oxford and IBH Publishing Company, New Delhi. 593 p
• Sood A, Sharma OP, Palni LMS (1992) Improved methods of propagation of maggar (Dendrocalamus hamiltonii Nees et Arn. Ex Munro) using single node cutting taken from juvenile culms of elite seedlings. J Am Bamboo Soc 9:17–24
• Sood A, Ahuja PS, Sharma M, Sharma OP, Godbole S (2002a) In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn. Ex Munro. Plant Cell Tissue Organ Cult 71:55–63. doi:10.1023/A: 1016582732531
• Sood A, Palni LMS, Sharma M, Chand G, Sharma OP (2002b) Micropropagation of Dendrocalamus hamiltonii Munro (Maggar Bamboo) using explants taken from seed raised and field-tested plus plants. J Plant Biol 29(2):125–132
• Virk PS, Ford-Llyod BV, Jackson MT, Newbury HJ (1995) Use of RAPD for the study of diversity within plant germplasm collections. Heredity 74:170–179. doi:10.1038/hdy.1995.25

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