The role of low molecular weight thiols in thimerosal toxicity - an in votro study

• Autorzy: Zieminska E. , Toczylowska B. , Wojcik J. , Stafiej A. , Bal W. , Lazarewicz J.
• Języki publikacji: EN

Abstrakty

EN

Thimerosal (TH), an ethylmercury complex of thiosalicylic acid has been used as preservative in vaccines. Inspired by a known high affi nity of mercury for thiol groups, we examined whether the presence of L-cysteine (Cys), D,L-homocysteine (Hcy), Nacetyl cysteine (NAC), L-methionine (Met) and glutathione (GSH) in extracellular space could infl uence the viability, intracellular calcium concentration ([Ca2+]i ) and mitochondrial membrane potential in rat cerebellar granule cells. The cells were exposed to 500 nM TH for 48 h or 15 μM TH for 10 min. The loss of cells viability could be prevented partially or wholly, in a dose-dependent manner, by 60, 120 or 600 μM Cys, Hcy, NAC and GSH, but not by Met. The elevation in [Ca2+]i and mitochondrial potential induced by 25 μM TH were abolished by all compounds studied, except for Met, at 600 μM. The loss of the ethylmercury moiety from TH as a result of interaction with thiols studied was monitored by 1 Hand 199Hg-NMR spectroscopy. This extracelullar process may be responsible for the neuroprotection seen in cerebellar cell culture, but also provides a molecular pathway for redistribution of TH derived toxic ethylmercury in the organism.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.316

Twórcy

autor

Zieminska E.

• Department of Neurochemistry, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Toczylowska B.

• Laboratory of Biological NMR, Warsaw, Poland

autor

Wojcik J.

• Laboratory of Biological NMR, Warsaw, Poland

autor

Stafiej A.

• Department of Neurochemistry, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Bal W.

• Department of Biophysics, Institute of Biochemistry and Biophysics, Warsaw, Poland

autor

Lazarewicz J.

• Department of Neurochemistry, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland