PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 31 | 6 |

Tytuł artykułu

Abscisic acid and methyl jasmonate as regulators of ethylene biosynthesis during somatic embryogenesis of Medicago sativa L.

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Effects of abscisic acid (ABA) and methyl jasmonate (MeJA) on ethylene production, ACC oxidase (ACO) activity, and content of 1-aminocyclopropane-1-carboxylic acid (ACC) during indirect somatic embryogenesis (SE) of Medicago sativa L. were studied. ABA and MeJA, at 50 µM, were applied during the induction, proliferation, or differentiation phase. ABA decreased ethylene production at the beginning of callus and SE induction and during the differentiation of somatic embryos. The hormone inhibited ACO activity in explants with overgrowing callus during the first two weeks of induction, in embryogenic suspension and also in differentiating embryos. The ACC content was reduced by ABA in callus at the end of SE induction, in embryogenic suspension and in globular embryos, but elevated in cotyledonary embryos. MeJA had no significant effect on ethylene production during M. sativa SE, despite the fact, that it inhibited ACO activity during the first two weeks of induction and in torpedo and cotyledonary embryos. The ACC content was increased by MeJA in 14-day-old callus and embryogenic suspension but was inhibited in globular embryos. Both ABA and MeJA seem to be involved in the regulation of ethylene biosynthesis during distinct phases of SE in M. sativa. It might be considered that exogenous ABA, more probably than MeJA, exerts its inhibitory effect on M. sativa somatic embryo formation by modifying ethylene production.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

31

Numer

6

Opis fizyczny

p.1263-1270,fig.,ref.

Twórcy

  • Department of Plant Physiology and Biotechnology, University of Szczecin, Waska 13, 71-415 Szczecin, Poland
autor
  • Department of Plant Physiology and Biotechnology, University of Szczecin, Waska 13, 71-415 Szczecin, Poland
  • Department of Plant Physiology and Biotechnology, University of Szczecin, Waska 13, 71-415 Szczecin, Poland

Bibliografia

  • Anandarajah K, McKersie BD (1990) Manipulating the desiccation tolerance and vigor of dry somatic embryos of Medicago sativa L. with sucrose, heat shock and abscisic acid. Plant Cell Rep 9:451–455
  • Atanassov A, Brown DCW (1984) Plant regeneration from suspension cultures and mesophyll protoplasts of Medicago sativa L. Plant Cell Tiss Org Cult 3:149–162
  • Bingham ET, Hurley LV, Kaatz DM, Saunders JW (1975) Breeding alfalfa which regenerate from callus tissue culture. Crop Sci 15:719–721
  • Dudits D, Gyorgyey J, Bogre L, Bako L (1995) Molecular biology of somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plant. Kluwer Academic Publishers, Dordrecht, pp 264–309
  • Evans PT, Malmberg LM (1989) Do polyamines have roles in plant development? Annu Rev Plant Physiol Plant Mol Biol 40:235–269
  • Frankowski K, Kęsy J, Wojciechowski W, Kopcewicz J (2009) Lightand IAA-regulated ACC synthase gene (PnACS) from pharbitis nil and its possible role in IAA-mediated flower inhibition. J Plant Physiol 166:192–202
  • Gamborg OL, Miller RA, Oijma K (1968) Nutrient requirement of suspension cultures of soybean root cell. Exp Cell Res 50:148–151
  • Hoon CY, Lee MM, Lee SH, Chung Y (1997) Effects of methyl jasmonate (MeJA) on the dark-induced senescence in oat (Avena sativa L.) leaf segments. J Plant Biol 40:9–14
  • Huang XL, Li XJ, Li Y, Huang LZ (2001) The effect of AOA on ethylene and polyamine metabolism during early phases of somatic embryogenesis in Medicago sativa. Physiol Plant 113:424–429
  • Kende H, Zeevaart JAD (1997) The five ‘‘Classical’’ plant hormones. Plant Cell 9:1197–1210
  • Kępczyńska E, Zielińska S (2006) Regulation of Medicago sativa L. somatic embryos regeneration by gibberellin A₃ and abscisic acid in relation to starch content and α-amylase activity. Plant Growth Regul 49:209–217
  • Kępczyńska E, Ruduś I, Kępczyński J (2009) Endogenous ethylene in indirect somatic embryogenesis of Medicago sativa L. Plant Growth Regul 59:63–73
  • Kępczyński J, Florek I (1997) The influence of JA-ME and ABA on induction of somatic embryogenesis in Medicago sativa L. In: Ellis RH, Black M, Murdoch AJ, Hong TD (eds) Basic and applied aspects of seed biology. Kluwer Academic Publishers, Dordrecht, pp 137–140
  • Kępczyński J, McKersie BD, Brown DCW (1992) Requirement of ethylene for growth of callus and somatic embryogenesis in Medicago sativa L. J Exp Bot 43:1199–1202
  • Kępczyński J, Białecka B, Kępczyńska E (1999) Ethylene biosynthesis in Amaranthus caudatus seeds in response to methyl jasmonate. Plant Growth Regul 28:59–65
  • Kępczyński J, Kępczyńska E, Bihun M (2003) The involvement of ethylene in the release of primary dormancy in Amaranthus retroflexus seeds. Plant Growth Regul 39:57–62
  • Kondo S, Meemak S, Ban Y, Moriguchi T, Harada T (2009) Effects of auxin and jasmonates on 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase gene expression during ripening of apple fruit. Postharv Biol Technol 51:281–284
  • Leung J, Giraudat J (1998) Abscisic acid signal transduction. Annu Plant Physiol Plant Mol Biol 49:199–222
  • Lizada MCC, Yang SF (1979) A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid. Ann Biochem 100: 140–145
  • McKeon T, Hoffman NE, Yang SF (1982) Effect of plant hormone pretreatments on ethylene production and synthesis of 1-aminocyclopropane-1-carboxylic acid in water stressed wheat leaves. Planta 155:437–443
  • McKersie BD, Senaratna T, Bowley SR, Brown DCW, Krochko F, Bewley JD (1989) Application of artificial seed technology in the production of hybrid alfalfa (Medicago sativa L.). In Vitro Cell Dev Biol 25:1183–1188
  • Meijer EGM (1989) Developmental aspects of ethylene biosynthesis during somatic embryogenesis in tissue cultures of Medicago sativa L. J Exp Bot 40:479–484
  • Müller R, Stummann BM, Andersen AS, Serek M (1999) Involvement of ABA in postharvest life of miniature potted roses. Plant Growth Regul 29:143–150
  • Pathier B, Brückner C, Dathe W, Hause B, Herrmann G, Knöfel HD, Kramell HM, Kramell R, Lehmann J, Miersch O, Reinbothe S, Sembdner G, Wasternack C, Zur Nieden U (1992) Jasmonates: metabolism, biological activities, and modes of action in senescence and stress responses. In: Karssen CM, Van Loon LC, Vreugdenhil D (eds) Progress in plant growth regulation. Kluwer Academic Publishers, Dordrecht, pp 276–285
  • Raghavan C, Ong EK, Dalling MJ, Stevenson TW (2006) Regulation of genes associated with auxin, ethylene and ABA pathways by 2, 4-dichlorophenoxyacetic acid in Arabidopsis. Funct Integr Genomics 6:60–70
  • Ruduś I, Kępczyńska E, Kępczyński J (2001) The influence of the jasmonates and abscisic acid on callus growth and somatic embryogenesis in Medicago sativa L. tissue culture. Acta Physiol Plant 23:103–107
  • Ruduś I, Kępczyńska E, Kępczyński J (2005) Changes in jasmonates and 12-oxophytodienoic acid contents of Medicago sativa L. during somatic embryogenesis. Acta Physiol Plant 27:317–324
  • Ruduś I, Kępczyńska E, Kepczyński J (2006) Comparative efficiency of abscisic acid and metyl jasmonate for indirect somatic embryogenesis in Medicago sativa L. Plant Growth Regul 48:1–11
  • Ruduś I, Weiler EW, Kępczyńska E (2009) Do stress-related phytohormones, abscisic acid and jasmonic acid play a role in the regulation of Medicago sativa L. somatic embryogenesis? Plant Growth Regul. doi:10.1007/s10725-009-9399-3
  • Senaratna T, McKersie BD, Bowley SR (1990) Artificial seeds of alfalfa (Medicago sativa L.). Induction of desiccation tolerance in somatic embryos. In Vitro Cell Dev Biol 26:85–90
  • Spollen WG, LeNoble ME, Samuels TD, Berstein N, Sharp RE (2000) Abscisic acid accumulation maintains maize primary root elongation at low water potentials by restricting ethylene production. Plant Physiol 122:967–976
  • Wasternack C, Hause B (2002) Jasmonates and octadecanoids: signals in plant stress responses and development. Prog Nucl Acid Res Mol Biol 72:165–221
  • Yoon IS, Park DH, Mori H, Imaseki H, Kang BG (1999) Characterisation of an auxin-inducible 1-aminocyclopropane-1-carboxylate synthase gene, VR-ACS6, of mungbean (Vigna radiata L cv. Wilczek) and hormonal interaction on the promoter activity in transgenic tobacco. Plant Cell Physiol 40:431–438

Uwagi

Rekord w opracowaniu

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-141bdb19-5e93-4192-a104-bba1bc10b6af
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.