Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enterocolitica O:9

• Autorzy: Weiner M. , Zlotnicka J. , Iwaniak W. , Szulowski K.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to develop a multiplex PCR, which allows an identification of the universal 16S rRNA Brucella sp. marker and amplification of the perosamine synthetase (per) gene, specific for cross-reacting Yersinia enterocolitica 0:9 only. The PCR analysis of the DNA extracted from all Brucella reference strains used in the investigations revealed the presence of the 16S rRNA gene, generating the amplicon of 905 bp size. Parallely, the examination of the DNA from Y. enterocolitica belonging to 0:9 serotype showed the presence of predicted amplicon of 312 bp typical to 0:9 serotype only. The sensitivity of the developed assay was determined with lymph tissue samples inoculated with B. abortus bvl and Y. enterocolitica 0:9 strains. The PCR analysis of the DNA extracted from lymph tissue revealed the presence of the predicted gene, when the 10⁶-10² CFU/g of the bacteria was added to the lymph tissue. The mPCR developed with direct extraction of DNA from lymph tissue can be a useful tool for the differentiation of infections caused by Brucella and cross-reacting Y enterocolitica 0:9.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 4
• Opis fizyczny: p.603-607,fig.,ref.

Twórcy

autor

Weiner M.

• Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zlotnicka J.

autor

Iwaniak W.

autor

Szulowski K.

Bibliografia

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