Comparison of two molecular methods for genotyping of Campylobacter sp. isolated from poultry

• Autorzy: Wieczorek K. , Osek J.
• Języki publikacji: EN

Abstrakty

EN

In the presented paper, PCR-RFLP and RFLP-PFGE with two different enzymes were applied for the discrimination of 48 Campylobacter isolates from poultry carcasses and faeces. Both used typing methods had a typeability of 100% on this collection of the strains. PFGE with SacII enzyme was the most discriminatory method with the discrimination index of 0.98. Restriction analysis of the flaA gene also appeared to be a suitable typing method because of rapidness and easy to perform especially in large-scale investigations. By the reason of some limitations of both tests in epidemiological studies, combined two molecular methods based on whole genome and fragment DNA analysis could provide the most relevant data for the identification and differentiation of Campylobacter sp. strains.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 4
• Opis fizyczny: p.523-528,fig.,ref.

Twórcy

autor

Wieczorek K.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Osek J.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Açik M.N., Cetinkaya B.: The heterogeneity of Campylobacter jejuni and Campylobacter coli strains isolated from healthy cattle. Lett Appl Microbiol 2005, 41, 397-403.
• 2. Altekruse S.F., Stern N.J., Fields P.I., Swerdlow D.L.: Campylobacter jejuni - an emerging foodborne pathogen. Emerg Infect Dis 1999, 5, 28-35.
• 3. Chuma T., Hashimoto S., Okamoto K.: Detection of thermophilic Campylobacter from sparrows by multiplex PCR: the role of sparrows as a source of contamination of broilers with Campylobacter. J Vet Med Sci 2000, 62, 1291-1295.
• 4. Endtz H.P., Ang C.W., van Den Braak N., Duim B., Rigter A,. Price L.J., Woodward D.L., Rodgers F.G., Johnson W.M., Wagenaar J.A., Jacobs B.C., Verbrugh H.A., van Belkum A.: Molecular characterization of Campylobacter jejuni from patients with Guillain-Barre and Miller Fisher syndromes. J Clin Microbiol 2000, 38, 2297-2301.
• 5. European Food Safety Authority: Third Community Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Antimicrobial resistance in the European Union in 2006.
• 6. Fitzgerald C., Stanley K., Andrew S., Jones K.: Use of pulsed-field gel electrophoresis and flagellin gene typing in identifying clonal groups of Campylobacter jejuni and Campylobacter coli in farm and clinical environments. Appl Environ Microbiol 2001, 67, 1429-1436.
• 7. Hanninen M.L., Pajarre S., Klossner M.L., Rautelin H.: Typing of human Campylobacter jejuni isolates in Finland by pulsed-field gel electrophoresis. J Clin Microbiol 1998, 36, 1787-1789.
• 8. Hanninen M.L., Perko-Makela P., Pitkala A., Rautelin H.: A three-year study of Campylobacter jejuni genotypes in humans with domestically acquired infections and in chicken samples from the Helsinki area. J Clin Microbiol 2000, 38, 1998-2000.
• 9. Harrington C.S., Moran L., Ridley A.M., Newell D.G., Madden R.H.: Inter-laboratory evaluation of three flagellin PCR/RFLP methods for typing Campylobacter jejuni and C. coli: the CAMPYNET experience. J Appl Microbiol 2003, 95, 1321-1333.
• 10. Harrington C.S., Thomson-Carter F.M., Carter P.E.: Evidence for recombination in the flagellin locus of Campylobacter jejuni: implications for the flagellin gene typing scheme. J Clin Microbiol 1997, 35, 2386-2392.
• 11. Hernandez J., Fayos A., Ferrus M.A., Owen R.J.: Random amplified polymorphic DNA fingerprinting of Campylobacter jejuni and C. coli isolated from human faeces, seawater and poultry products. Res Microbiol 1995, 146, 685-696.
• 12. http://campynet.vetinst.dk/Fla.htm
• 13. Hunter P.R., Gaston M. A.: Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 1988, 26, 2465-2466.
• 14. Ishihara K., Yamamoto T., Satake S., Takayama S., Kubota S., Negishi H., Kojima A., Asai T., Sawada T., Takahashi T., Tamura Y.: Comparison of Campylobacter isolated from humans and food-producing animals in Japan. J Appl Microbiol 2006, 100, 153-160.
• 15. Nachamkin I., Ung H., Patton C.M.: Analysis of HL and O serotypes of Campylobacter strains by the flagellin gene typing system. J Clin Microbiol 1996, 34, 277-281.
• 16. Nielsen E.M., Engberg J., Fussing V., Petersen L., Brogren C.H., On S. L.: Evaluation of phenotypic and genotypie methods for subtyping Campylobacter jejuni isolates from humans, poultry, and cattle. J Clin Microbiol 2000, 38, 3800-3810.
• 17. Petersen L., Newell D.G.: The ability of Fla-typing schemes to discriminate between strains of Campylobacter jejuni. J Appl Microbiol 2001, 91, 217- 224.
• 18. Petersen L., On S.L.: Efficacy of flagellin gene typing for epidemiological studies of Campylobacter jejuni in poultry estimated by comparison with macrorestriction profiling. Lett Appl Microbiol 2000, 31, 14-19.
• 19. Ribot E.M., Fitzgerald C., Kubota K., Swaminathan B., Barrett T.J.: Rapid pulsed-field gel electrophoresis protocol for subtyping of Campylobacter jejuni. J Clin Microbiol 2001, 39, 1889-1894.
• 20. Rivoal K., Ragimbeau C., Salvat G., Colin P., Ermel G.: Genomic diversity of Campylobacter coli and Campylobacter jejuni isolates recovered from free-range broiler farms and comparison with isolates of various origins. Appl Environ Microbiol 2005, 71, 6216-6227.
• 21. Tam C.C., O'Brien S.J., Adak G.K., Meakins S.M., Frost J.A.: Campylobacter coli - an important foodborne pathogen. J Infect 2003, 47, 28-32.
• 22. Versalovic J., Koeuth T., Lupski J.R.: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 1991, 19, 6823-6831.
• 23. Wassenaar T.M., Newell D.G.: Genotyping of Campylobacter spp. Appl Environ Microbiol 2000, 66, 1- 9.
• 24. Wieczorek K., Osek J.: Multiplex PCR assays for simultaneous identification of Campylobacter jejuni and Campylobacter coli. Medycyna Wet 2005, 61, 797-799.
• 25. www.cdc.gov/pulsenet/ protocols/campy_protocol.pdf
• 26. Yan W., Chang N., Taylor D.E.: Pulsed-field gel electrophoresis of Campylobacter jejuni and Campylobacter coli genomic DNA and its epidemiologic application. J Infect Dis 1991, 163, 1068-1072.