Single course of antenatal betamethasone produces delayed changes in morphology and calbindin-D28k expression in a rat’s cerebellar Purkinje cells

• Autorzy: Pascual R. , Valencia M. , Larrea S. , Bustamante C.
• Języki publikacji: EN

Abstrakty

EN

In the current study, we analyzed the impact of antenatal betamethasone on macroscopic cerebellar development, Purkinje cell morphology and the expression of the neuroprotective protein calbindin-D28k. Pregnant rats (Sprague-Dawley) were randomly divided into two experimental groups: control (CONT) and betamethasone-treated (BET). At gestational day 20 (G20), BET dams were subcutaneously injected with a solution of 0.17 mg kg-1 of betamethasone, while CONT animals received a similar volume of saline. At postnatal days 22 (P22) and P52, BET and CONT offspring were behaviorally evaluated, and the cerebella were histologically and immunohistochemically processed. Animals that were prenatally treated with a single course of betamethasone exhibited long-lasting behavioral changes consistent with anxiety-like behavior in the open-field test, together with (1) reduced cerebellar weight and volume, (2) Purkinje cell dendritic atrophy, and (3) an overexpression of calbindin-D28k. The current results indicate that an experimental single course of betamethasone in pregnant rats produces long-lasting anxiety-like behaviors, together with macroscopic and microscopic cerebellar alterations.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2014
• Tom: 74
• Numer: 4
• Opis fizyczny: p.415-423,fig.,ref.

Twórcy

autor

Pascual R.

• Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaíso, Valparaíso, Chile

autor

Valencia M.

• Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaíso, Valparaíso, Chile

autor

Larrea S.

• Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaíso, Valparaíso, Chile

autor

Bustamante C.

• Laboratorio de Neurociencias, Escuela de Kinesiología, Facultad de Ciencias, Pontificia Universidad Catolica de Valparaíso, Valparaíso, Chile

Bibliografia

• Ahlbom E, Gogvadze V, Chen M, Celsi G, Ceccatelli S (2000) Prenatal exposure to high levels of glucocorticoids increases the susceptibility of cerebellar granule cells to oxidative stress-induced cell death. Proc Natl Acad Sci U S A 97: 14726-14730.
• Airaksinen MS, Eilers J, Garaschuk O, Thoenen H, Konnerth A, Meyer M (1997) Ataxia and altered dendritic calcium signaling in mice carrying a targeted null mutation of the calbindin-D28k gene. Proc Natl Acad Sci U S A 94: 1488-1493.
• Allin M, Matsumoto H, Santhouse AM, Nosarti C, Al Asady MH, Stewart AL, Rifkin L, Murray RM (2001) Cognitive and motor function and the size of the cerebellum in ado¬lescents born very pre-term. Brain 124: 60-66.
• Arahuetes RM, Carretero V, Diebold Y, Rua C (1991) Effects of maternal bilateral adrenalectomy and betamethasone administration on fetal rat encephalic develop¬ment. Biol Neonate 59: 303-313.
• Barski JJ, Hartmann J, Rose CR, Hoebeek F, Morl K, Noll- Hussong M, De Zeeuw CI, Konnerth A, Meyer M (2003) Calbindin in cerebellar Purkinje cells is a critical determi¬nant of the precision of motor coordination. J Neurosci 23: 3469-3477. Bastianelli E (2003) Distribution of calcium-binding pro¬teins in the cerebellum. Cerebellum 2: 242-262.
• Bolduc ME, Du Plessis AJ, Evans A, Guizard N, Zhang X, Robertson RL, Limperopoulos C (2011) Cerebellar mal¬formations alter regional cerebral development. Dev Med Child Neurol 53: 1128-1134.
• Bruschettini M, van den Hove DL, Timmers S, Welling M, Steinbusch HP, Prickaerts J, Gazzolo D, Blanco CE, Steinbusch HW (2006) Cognition- and anxiety-related behavior, synaptophysin and MAP2 immunoreactivity in the adult rat treated with a single course of antenatal betamethasone. Pediatr Res 60: 50-54.
• Clancy B, Finlay BL, Darlington RB, Anand KJ (2007) Extrapolating brain development from experimental spe¬cies to humans. Neurotoxicology 28: 931-937.
• Ginath S, Lerman-Sagie T, Haratz Krajden K, Lev D, Cohen-Sacher B, Bar J, Malinger G (2013) The fetal ver¬mis, pons and brainstem: normal longitudinal develop¬ment as shown by dedicated neurosonography. J Matern Fetal Neonatal Med 26: 757-762.
• Huang WL, Beazley LD, Quinlivan JA, Evans SF, Newnham JP, Dunlop SA (1999) Effect of corticosteroids on brain growth in fetal sheep. Obstet Gynecol 94: 213-218.
• Iacopino AM, Christakos S (1990) Corticosterone regulates calbindin-D28k mRNA and protein levels in rat hip¬pocampus. J Biol Chem 265: 10177-10180.
• Krugers HJ, Medema RM, Postema F, Korf J (1995) Region- specific alterations of calbindin-D28k immunoreactivity in the rat hippocampus following adrenalectomy and cor- ticosterone treatment. Brain Res 696: 89-96.
• Lavezzi AM, Ottaviani G, Terni L, Matturri L (2006) Histological and biological developmental characteriza¬tion of the human cerebellar cortex. Int J Dev Neurosci 24: 365-371.
• Miller SL, Chai M, Loose J, Castillo-Meléndez M, Walker DW, Jenkin G, Wallace EM (2007) The effects of maternal betamethasone administration on the intrauterine growth-restricted fetus. Endocrinology 148: 1288-1295.
• Pascual R, Ebner D, Araneda R, Urqueta MJ, Bustamante C (2010) Maternal stress induces long-lasting Purkinje cell developmental impairments in mouse offspring. Eur J Pediatr 169: 1517-1522.
• Pascual R, Bustamante C (2013). Early postweaning social isolation but not environmental enrichment modifies ver¬mal Purkinje cell dendritic outgrowth in rats. Acta Neurobiol Exp (Wars) 73: 387-393.
• Pascual R, Zamora-León P, Bustamante C (2013) Selegiline (deprenyl) decreases calbindin-D28k expression in corti¬cal neurons of rats socially deprived during the post- weaning period. Int J Dev Neurosci 31: 145-149.
• Pavlík A, Buresová M (1984) The neonatal cerebellum: the highest level of glucocorticoid receptors in the brain. Brain Res 314: 13-20.
• Rebolledo-Solleiro D, Crespo-Ramírez M, Roldán- Roldán G, Hiriart M, Pérez de la Mora M (2013) Role of thirst and visual barriers in the differential behavior displayed by streptozotocin-treated rats in the elevated plus-maze and the open field test. Physiol Behav 120: 130-135.
• Rinaudo P, Wang E (2012) Fetal programming and meta¬bolic syndrome. Annu Rev Physiol 74: 107-130.
• Sacchetti B, Scelfo B, Strata P (2009) Cerebellum and emo¬tional behavior. Neuroscience 162: 756-762.
• Shah DK, Anderson PJ, Carlin JB, Pavlovic M, Howard K, Thompson DK, Warfield SK, Inder TE (2006) Reduction in cerebellar volumes in preterm infants: relationship to white matter injury and neurodevelopment at two years of age. Pediatr Res 60: 97-102.
• Scheepens A, van de Waarenburg M, van den Hove D, Blanco CE (2003) A single course of prenatal betametha- sone in the rat alters postnatal brain cell proliferation but not apoptosis. J Physiol 552: 163-175.
• Spinillo A, Viazzo F, Colleoni R, Chiara A, Maria Cerbo R, Fazzi E (2004) Two-year infant neurodevelopmental out¬come after single or multiple antenatal courses of corti- costeroids to prevent complications of prematurity. Am J Obstet Gynecol 191: 217-224.
• Spittle AJ, Doyle LW, Anderson PJ, Inder TE, Lee KJ, Boyd RN, Cheong JL. (2010) Reduced cerebellar diameter in very preterm infants with abnormal general movements. Early Hum Dev 86: 1-5.
• Strata P, Scelfo B, Sacchetti B (2011) Involvement of cere¬bellum in emotional behavior. Physiol Res 60 Suppl 1: S39-48.
• Tam EW, Chau V, Ferriero DM, Barkovich AJ, Poskitt KJ, Studholme C, Fok ED, Grunau RE, Glidden DV, Miller SP (2011) Preterm cerebellar growth impairment after postnatal exposure to glucocorticoids. Sci Transl Med 3: 105ra-105. [doi: 10.1126/scitranslmed.3002884]
• Velazquez PN, Romano MC (1987) Corticosterone therapy during gestation: effects on the development of rat cere¬bellum. Int J Dev Neurosci 5: 189-194.
• Wójcik S, Łuczyńska A, Dziewiatkowski J, Spodnik E, Ludkiewicz B, Moryś J (2013) Expression of the calci¬um-binding proteins in the central, medial and cortical nuclei of the rabbit amygdaloid complex during post¬natal development. Acta Neurobiol Exp (Wars) 73: 260-279.