Effects of ten traditional Chinese herbs on immune response and disease resistance of Sciaenops ocellatus (Actinopterygii: Perciformes: Sciaenidae)

• Autorzy: Pan T. , Yan M. , Chen S. , Wang X.
• Języki publikacji: EN

Abstrakty

EN

Background. Traditional Chinese herbs (TCHs) are widely used for improving non-specific immunity of fish in aquaculture. Possible effects of many important TCH on red drum, Sciaenops ocellatus (Linnaeus, 1766), have not been adequately studied. The aim of the presently reported study was to investigate the effects of ten TCH on selected immunological- and haematological responses of red drum. Materials and Methods. >Ten TCH preparations, consisting of selected parts of plants and fungi, known in medicinal practice as: Astragalus membranaceus, fructus forsythiae, Polyporus umbellatus, Scutellaria baicalensis, Wolfiporia extensa, rhizoma coptidis, radix glycyrrhizae, flos lonicerae japonicae, isatidis radix, and bupleuri radix were used in the experiment. A dose of 10 g of each TCH was decocted and concentrated, then mixed with the diets (mixed diets) at ratio of 2%. After 28 days of feeding, the fish were infected with Vibrio splendidus and observed for 14 days for possible mortalities. Leukocyte phagocytic activity and lysozyme activity (LZM) were measured on days 0, 3, 7, 14, 21, 28, and 35. Relative percentage survival (RPS) of each TCH treatment was also investigated after the fish exposure to V. splendidus. Results. Red drum fed 2% doses of individual TCH extracts significantly (P < 0.05) increased its: phagocytic percentage (PP) (with the exception of flos lonicerae japonicae treatment), phagocytic index (PI) (with the exception of flos lonicerae japonicae- and bupleuri radix treatments), LZM (with the exception of Wolfiporia extensa treatment), and RPS (with the exception of P. umbellatus, Wolfiporia extensa-, radix glycyrrhizae-, flos lonicerae japonicae-, isatidis radix-, and bupleuri radix treatments). The indexes of PP, PI, and LZM, for all experimental groups, decreased quickly on day 35 (7 days after TCH diets were discontinued). On the other hand, PP values were significantly (P < 0.05) higher than in control (with the exception of flos lonicerae japonicae- and Wolfiporia extensa treatments). Also PI values were significantly higher (P < 0.05) than in control (with the exception of flos lonicerae japonicae-, Wolfiporia extensa-, and bupleuri radix treatments). LZM values exhibited no significant difference (P > 0.05) in all treatment groups compared with control. Most importantly, the A. membranaceus-, or S. baicalensis-, or fructus forsythiae-fed groups were significantly protected (P < 0.01) against V. splendidus challenge compared to control. RPS of A. membranaceus- and S. baicalensis groups were the highest, reaching 88.9% on day 28, followed by the fructus forsythiae group, whereas bupleuri radix- and Wolfiporia extensa treatments showed the lowest values. Conclusion. A. membranaceus, S. baicalensis, and fructus forsythiae were effective in preventing red drum from acquiring the disease while challenged by V. splendidus.

• Wydawca: -
• Czasopismo: Acta Ichthyologica et Piscatoria
• Rocznik: 2013
• Tom: 43
• Numer: 1
• Opis fizyczny: p.41-49,fig.,ref.

Twórcy

autor

Pan T.

• College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China
• Fisheries Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui Province 230031, China

autor

Yan M.

• Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou, Zhejiang Province 325005, China

autor

Chen S.

• Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Wenzhou, Zhejiang Province 325005, China

autor

Wang X.

• College of Animal Science and Technology, Shandong Agricultural University, Taian, Shangdong Province, 271018, China

Bibliografia

• Abd-El-Rhman A.M.M. 2009. Antagonism of Aeromonas hydrophila by propolis and its effect on the performance of Nile tilapia, Oreochromis niloticus.Fish and Shellfish Immunology 27 (3): 454–459. DOI:10.1016/j.fsi.2009.06.015
• Ahmadi K., Banaee M., Vosoghei A.R., Mirvaghefei A.R.,Ataeimehr B. 2012. Evaluation of the immunomodulatory effects of silymarin extract (Silybum marianum) on some immune parameters of rainbow trout,Oncorhynchus mykiss (Actinopterygii:Salmoniformes: Salmonidae) Acta Ichthyologica et Piscatoria 42 (2): 113–120. DOI: 10.3750/aip2011.42.2.04
• Ai Q., Mai K., Zhang L., Tan B., Zhang W., Xu W., Li H.2007. Effects of dietary β-1, 3 glucan on innate immune response of large yellow croaker,Pseudosciaena crocea. Fish and Shellfish Immunology 22 (4): 394–402. DOI: 10.1016/j.fsi.2006.06.011
• Anderson D.P. 1992. Immunostimulants, adjuvants, and vaccine carriers in fish: Applications to aquaculture.Annual Review of Fish Diseases 2: 281–307. DOI:10.1016/0959-8030(92)90067-8
• Ardó L., Yin G., Xu P., Váradi L., Szigeti G., Jeney Z.,Jeney G. 2008. Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Aquaculture 275 (1–4): 26–33.DOI: 10.1016/j.aquaculture.2007.12.022
• Bakopoulos V., Volpatti D., Gusmani L., Galeotti M.,Adams A., Dimitriadis G.J. 2003. Vaccination trias of sea bass, Dicentrarchus labrax (L.), against Photobacterium damsela subsp. piscicida, using novel vaccine mixtures. Journal of Fish Diseases 26 (2):77–90. DOI: 10.1046/j.1365-2761.2003.00438.x
• Brunt J., Newaj-Fyzul A., Austin B. 2007. The development of probiotics for the control of multiple bacterial diseases of rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Fish Diseases 30 (10):573–579. DOI: 10.1111/j.1365-2761.2007.00836.x
• Chen X., Wu Z., Yin J., Li L. 2003. Efects of four species of herbs on immune function of Carassiusauratus gibelio. Journal of Fishery Sciences of China 10 (1): 36–40.
• Diamant A. 1998. Red drum Sciaenops ocellatus (Sciaenidae), a recent introduction to Mediterranean mariculture, is susceptible to Myxidium leei (Myxosporea). Aquaculture 162 (1–2): 33–39. DOI:10.1016/S0044-8486(97)00307-4
• Galina J., Yin G., Ardó L., Jeney Z. 2009. The use of immunostimulating herbs in fish. An overview of research. Fish Physiology and Biochemistry 35 (4):669–676. DOI: 10.1007/s10695-009-9304-z
• Harikrishnan R., Kim J.-S., Kim M.-C.,Balasundaram C., Heo M.-S. 2011. Hericium erinaceum enriched diets enhance the immune response in Paralichthys olivaceus and protect from Philasterides dicentrarchi infection. Aquaculture 318 (1–2): 48–53. DOI: 10.1016/j.aquaculture.2011.04.048
• Huang C.W., Lee T.T., Shih Y.C., Yu B. 2011. Effects of dietary supplementation of Chinese medicinal herbs on polymorphonuclear neutrophil immune activity and small intestinal morphology in weanling pigs.Journal of Animal Physiology and Animal Nutrition 96 (2): 285–294. DOI: 10.1111/j.1439-0396.2011.01151.x
• Jeney G., Galeotti M., Volpatti D., Jeney Z., Anderson D.P.1997. Prevention of stress in rainbow trout (Oncorhynchus mykiss) fed diets containing different doses of glucan. Aquaculture 154 (1): 1–15. DOI:10.1016/S0044-8486(97)00042-2
• Jeney G., Jeney Z. 2002. Application of immunostimulants for modulation of the non-specific defense mechanisms in sturgeon hybrid: Acipenser ruthenus ×A. baerii. Journal of Applied Ichthyology 18 (4–6):416-419. DOI: 10.1046/j.1439-0426.2002.00405.x
• Jian J., Wu Z. 2003. Effects of traditional Chinese medicine on nonspecific immunity and disease resistance of large yellow croaker, Pseudosciaena crocea (Richardson). Aquaculture 218 (1–4): 1–9. DOI: 10.1016/S0044-8486(02)00192-8
• Lou L., Chen X., Cai X. 2001. Influence of cerat (Andrographis paniculata) on the phagocytic activity of phagocytes in grass carp. Journal of Southwest Agricultural University 23 (1): 33–34.
• Luo R. 1997. Induction of immunity substance In Penaeus chinensis by Chinese herbal medicine.Oceanologia et Limnologia Sinica 28 (6): 577–585.
• Ma A.-m., Yan M.-c., Chang W.-s., Xie Q.-l., Chen S.-b.,Shan L.-z?. 2009. Effects of Chinese herbs on the growth and immune function in Sciaenops ocellatus.Marine Sciences 33 (12): 96–102.
• Neumann N.F., Stafford J.L., Barreda D., Ainsworth A.J.,Belosevic M. 2001. Antimicrobial mechanisms of fish phagocytes and their role in host defense.Developmental and Comparative Immunology 25 (8–9): 807–825. DOI: 10.1016/S0145-305X(01)00037-4
• Reed L.J., Muench H. 1938. A simple method of estimating fifty per cent endpoints. American Journal of Epidemiology 27 (3): 493–497.
• Sakai M. 1999. Current research status of fish immunostimulants.Aquaculture 172 (1–2): 63–92. DOI:10.1016/S0044-8486(98)00436-0
• Seeley K.R., Gillespie P.D., Weeks B.A. 1990. A simple technique for the rapid spectrophotometric determination of phagocytosis by fish macrophages. Marine Environmental Research 30 (1): 37–41. DOI:10.1016/0141-1136(90)90009-D
• Wang J.Q., Sun Y., Zhang J. 2006. Effects of Chinese herb additives on growth, digestive activity and nonspecific immunity in flounder Paralichthys olivaceus.Journal of Fisheries of China 30 (1): 90–96.
• Wang X., Yan M., Chai X., Wang Q. 2012. Water decoction of Astragalus membranaceus enhances cellular immune response and disease resistance in spotted maigre. African Journal of Biotechnology 11 (55):11851–11856. DOI: 10.5897/AJB12.1375
• Wu C.-C., Liu C.-H., Chang Y.-P., Hsieh S.-L. 2010.Effects of hot-water extract of Toona sinensis on immune response and resistance to Aeromonas hydrophila in Oreochromis mossambicus. Fish and Shellfish Immunology 29 (2): 258–263. DOI:10.1016/j.fsi.2010.04.021
• Yan M., Chen S., Shan L., Ma A., Xie Q., Dai S., Li S. 2010. Selection of 22 kinds of Chinese herbal medicines on bacteriostasis in vitro on Vibrio vulnificus and Vibrio splendidus. Journal of Hydroecology 2: 95–98.
• Yin G., Ardó L., Thompson K.D., Adams A., Jeney Z.,Jeney G. 2009. Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophila. Fish and Shellfish Immunology 26 (1): 140–145. DOI: 10.1016/j.fsi.2008.08.015
• Yin G., Jeney G., Racz T., Xu P., Jun X., Jeney Z. 2006.Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus. Aquaculture 253 (1–4):39–47. DOI: 10.1016/j.aquaculture.2005.06.038
• Zhang G., Gong S., Yu D., Yuan H. 2009. Propolis and Herba Epimedii extracts enhance the non-specific immune response and disease resistance of Chinese sucker, Myxocyprinus asiaticus. Fish and Shellfish Immunology 26 (3): 467–472. DOI: 10.1016/j.fsi.2009.01.011

Uwagi

rekord w opracowaniu