PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2011 | 14 | 2 |
Tytuł artykułu

The influence of porcine pancreas digestion parameters and islet histomorphology on islet isolation outcome

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Transplantation of the pig islets of Langerhans is considered as the future treatment for patients suffering from type I diabetes mellitus. Despite the adaptation of modified Ricordi method and highly purified collagenase, the results of pancreas digestions are precarious. Selection of proper donor and optimal digestion procedure are fundamental. The aim of this study was to assess the impact of pancreas procuring parameters on pig islets yield. The pancreata were harvested from 69 market sows weighting over 150 kg. After intraductal injection of cold collagenase solution pancreata were transported in UW solution or under conditions of two layer method (TLM). In laboratory pancreata were digested at 37℃ according to Ricordi isolation method or stationary in the bottle. The particular parameters of isolation procedure were considered as substantial. Pig weight, volume of infused collagenase solution, TLM application and pancreas dividing before digestion positively affected islet yield. Additionally, the influence of pancreatic islet tissue histomorphology on isolation outcome was studied. Proper donor selection as well as adequate digestion parameters could improve pig islet recovery during islet isolation.
Wydawca
-
Rocznik
Tom
14
Numer
2
Opis fizyczny
p.227-230,fig.,ref.
Twórcy
  • Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland
autor
autor
autor
autor
Bibliografia
  • Calafiore R (2003) Alginate microcapsules for pancreatic islet cell graft immunoprotection: struggle and progress towards the final cure for type 1 diabetes mellitus. Expert Opin Biol Ther 3: 201-205.
  • De Vos P, De Haan BJ, Wolters GH, Strubbe JH, Van Schilfgaarde R (1997) Improved biocompatibility but limited graft survival after purification of alginate for microencapsulation of pancreatic islets. Diabetologia 40: 262-270.
  • Dufrane D, Goebbels RM, Fdilat I, Guiot Y, Gianello P (2005) Impact of porcine islet size on cellular structure and engraftment after transplantation: adult versus young pigs. Pancreas 30: 138-147.
  • Khan MH, Harlan DM (2009) Counterpoint: clinical islet transplantation: not ready for prime time. Diabetes Care 32: 1570-1574.
  • Kim JH, Kim HI, Lee KW, Yu JE, Kim SH, Park HS, Park CG, Ihm SH, Ha J, Kim SJ, Lee HK, Ahn C, Park KS (2007) Influence of strain and age differences on the yields of porcine islet isolation: extremely high islet yields from SPF CMS miniature pigs. Xenotransplantation 14: 60-66.
  • Krickhahn M, Buhler C, Meyer T, Thiede A, Ulrichs K (2002) The morphology of islets within the porcine donor pancreas determines the isolation result: successful isolation of pancreatic islets can now be achieved from young market pigs. Cell Transplant 11: 827-838.
  • Lim F, Sun AM (1980) Microencapsulated islets as bioartificial endocrine pancreas. Science 210: 908-910.
  • Merani S, Shapiro AM (2006) Current status of pancreatic islet transplantation. Clin Sci (Lond) 110: 611-625.
  • O’Neil JJ, Stegemann JP, Nicholson DT, Gagnon KA, Solomon BA, Mullon CJ (2001) The isolation and function of porcine islets from market weight pigs. Cell Transplant 10: 235-246.
  • Sabat M, Godlewska E, Kinasiewicz J, Urbanowicz A, Orłowski T (2003) Assessment of some porcine strains as donors of islets of Langerhans. Transplant Proc 35: 2343-2344.
  • Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL, Kneteman NM, Rajotte RV (2000) Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 343: 230-238.
  • Socci C, Ricordi C, Davalli AM, Staudacher C, Baro P, Vertova A, Freschi M, Gavazzi F, Braghi S, Pozza G (1990)
  • Selection of donors significantly improves pig islet isolation yield. Horm Metab Res Suppl 25: 32-34.
  • Tibell A, Groth CG, Moller E, Korsgren O, Andersson A, Hellerstrom C (1994) Pig-to-human islet transplantation in eight patients. Transplant Proc 26: 762-763.
  • Toso C, Brandhorst D, Oberholzer J, Triponez F, Buhler L, Morel P (2000) Isolation of adult porcine islets of Langerhans. Cell Transplant 9: 297-305.
  • Ulrichs K, Bosse M, Wacker HH, Heiser A, Muller-Ruchholtz W (1994) Histologic analysis of the porcine pancreas to improve islet yield and integrity after collagenase digestion. Transplant Proc 26: 610-612.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-12ec7f3f-9f61-4b77-87b0-053da91d51ad
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.