The lectin-binding pattern of nucleolin and its interaction with endogenous galectin-3

• Autorzy: Hoja-Lukowicz D. , Kedracka-Krok S. , Duda W. , Litynska A.
• Języki publikacji: EN

Abstrakty

EN

Unlike nuclear nucleolin, surface-expressed and cytoplasmic nucleolin exhibit Tn antigen. Here, we show localization-dependent differences in the glycosylation and proteolysis patterns of nucleolin. Our results provide evidence for different paths of nucleolin proteolysis in the nucleus, in the cytoplasm, and on the cell surface. We found that full-length nucleolin and some proteolytic fragments coexist within live cells and are not solely the result of the preparation procedure. Extranuclear nucleolin undergoes N- and O-glycosylation, and unlike cytoplasmic nucleolin, membrane-associated nucleolin is not fucosylated. Here, we show for the first time that nucleolin and endogenous galectin-3 exist in the same complexes in the nucleolus, the cytoplasm, and on the cell surface of melanoma cells. Assessments of the interaction of nucleolin with galectin-3 revealed nucleolar co-localization in interphase, suggesting that galectin-3 may be involved in DNA organization and ribosome biogenesis.

Słowa kluczowe

EN

lectin binding pattern; nucleolin; interaction; endogenous galectin-3

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2014
• Tom: 19
• Numer: 3
• Opis fizyczny: p.461-482,fig.,ref.

Twórcy

autor

Hoja-Lukowicz D.

• Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, 9 Gronostajowa Street, 30-387, Krakow, Poland

autor

Kedracka-Krok S.

• Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 7 Gronostajowa Street, 30-387 Krakow, Poland
• Malopolska Centre of Biotechnology, Jagiellonian University, 7 Gronostajowa Street, 30–387 Krakow, Poland

autor

Duda W.

• Laboratory of Neuropsychology, Departament of Neurophysiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, 02-093 Warszawa, Poland

autor

Litynska A.

• Department of Glycoconjugate Biochemistry, Institute of Zoology, Jagiellonian University, 9 Gronostajowa Street, 30-387, Krakow, Poland

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