Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 34 | 3 |

Tytuł artykułu

Membrane permeability and micro- and macroelement accumulation in spring wheat cultivars during the short-term effect of salinity- and PEG-induced water stress

Warianty tytułu

Języki publikacji



The comparative responses of ten spring wheat cultivars to water stress were investigated. Wheat plants were cultured under hydroponics conditions (Hoagland nutrient) to the stage of three-leaf seedlings. Then, the water medium was supplemented with PEG (drought) or NaCl (salinity) to obtain a water status equal to -1.5 MPa. After a 2-day treatment, the changes in the following parameters were determined: fresh and dry weight, macroand microelement accumulation, membrane injury (electrolyte leakage, lipid peroxidation) and fatty acid content of the phospholipid fraction of plasmalemma (in comparison to plants not stressed, taken as a control). Generally, the plants were more significantly influenced by water stress stimulated by PEG than by NaCl treatment, as compared to the plants cultivated in the control media. The results of the decrease in water content in leaves and electrolyte leakage from cells corresponded well with the intensity of lipid peroxidation (determined by malondialdehyde—MDAcontent) and were chosen for the selection of investigated genotypes for tolerance to both stresses. The more tolerant genotypes exhibited the opposite changes in phospholipid fatty acid unsaturation for two applied stresses i.e. NaCl treatment caused a decrease in unsaturation whereas in PEG-treated plants an increase in unsaturation was observed. These changes were reversed for less tolerant plants, i.e. NaCl treatment influenced an increase in fatty acid unsaturation whereas in PEG-treated plants a decrease in unsaturation was measured. The ratio of U/S (unsaturated to saturated fatty acids) correlated with the total amount of accumulated macroelements. The content of Mg, Ca and S in leaves of plants undergoing both stress factors (NaCl and PEG) dropped whereas the K and P content increased in leaves of wheat seedlings cultured on media containing NaCl only. For microelements, a decrease in the accumulation of these nutrients was detected in all investigated seedlings. However, a greater reduction in the level of these elements occurred in seedlings grown on media with PEG in comparison to those grown on NaCl containing media.

Słowa kluczowe








Opis fizyczny



  • Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland
  • Department of Chemistry, Jagiellonian University, Ingardena 3, 30-360 Krakow, Poland
  • Department of Chemistry, Jagiellonian University, Ingardena 3, 30-360 Krakow, Poland
  • Institute of Biology, Pedagogical University, Podbrzezie 3, 31-054 Krakow, Poland
  • Institute of Biology, Pedagogical University, Podbrzezie 3, 31-054 Krakow, Poland
  • Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland


  • Allakhverdiev SI, Kinoshita M, Inaba M, Suzuki I, Murata N (2001) Unsaturated fatty acids in membrane lipids protect the photosynthetic machinery against salt-induced damage in Synechoccus. Plant Physiol 125:1842–1853
  • Asada K (1999) The water–water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons. Ann Rev Plant Physiol Plant Mol Biol 50:601–639
  • Ashraf M, Ali Q (2008) Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L.). Environ Exp Bot 63:266–273
  • Ben Ammar W, Nouairi I, Zarrouk M, Jemal F (2007) Cadmium stress induces changes in the lipid composition and biosynthesis in tomato (Lycopersicon esculentum Mill.) leaves. Plant Growt Regul 53:75–85
  • Bligh EG, Dyer WJ (1959) A rapid method of total lipids extraction and purification. Can J Biochem 37:911–915
  • Block MA, Dorne AJ, Joyard R, Douce R (1983) Preparation and characterization of membrane fraction enriched in outer and inner envelope membranes from spinach chloroplasts. J Biol Chem 258:13281–13286
  • Dhindsa RS, Plumb-Dhindsa P, Thorpe TA (1981) Leaf senescence correlation with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J Exp Bot 32:96–101
  • Farooq S, Azam F (2006) The use of cell membrane stability (CMS) technique to stress for salt tolerant wheat varieties. J Plant Physiol 163:629–637
  • Filek M, Zembala M, Kornaś A, Walas S, Mrowiec H, Hartikainen H (2010) The uptake and translocation of macro- and microelements in rape and wheat seedlings as affected by selenium supply level. Plant Soil 336:303–312
  • Franco JA, Esteban C, Rodriguez C (1993) Effects of salinity on various growth stages of muskmelon cv. Revigal. J Hortic Sci 68:899–904
  • Guerfel M, Baccouri O, Boujnah D, Zarrouk M (2008) Changes in lipid composition, water relations and gas exchange in leaves of two young ‘Chemlali’ and ‘Chetoui’ olive trees in response to water stress. Plant Soil 311:121–129
  • Han LP, Steinberger Y, Zhao YL, Xie GH (2011) Accumulation and partitioning of nitrogen, phosphorus and potassium in different varieties of sweet sorghum. Field Crop Res 120:230–240
  • Hsiao T (1973) Plant responses to water stress. Ann Rev Plant Physiol 24:519–570
  • Hu Y, Burucs Z, von Tucher S, Schmidhalter U (2007) Short-term effects of drought and salinity on mineral nutrient distribution along growing leaves of maize seedlings. Environ Exp Bot 60:268–275
  • Kramer PJ (1988) Measurement of plant water status: historical perspectives and current concerns. Irrig Sci 9:275–287
  • Liang XZ, Ding SW, Wong SM (2002) Development of a kenaf (Hibiscus cannabinus L.) protoplast system for a replication study of Hibiscus chlorotic rings pot virus. Plant Cell Rep 20:982–986
  • Martyniak L, Dabrowska-Zielnska K, Szymczyk R, Gruszczynska M (2007) Validation of satellite-derived soil-vegetation indices for prognosis of spring cereals yield reduction under drought conditions—case study from central-western Poland. Adv Space Res 39:67–72
  • Munns R (1993) Physiological processes limiting plant growth in saline soils: some dogmas and hypothesis. Plant Cell Environ 16:201–218
  • Munns R (2002) Comparative physiology of salt and water stress. Plant Cell Environ 25:239–250
  • Nagy Z, Galiba G (1995) Drought and salt tolerance are not necessarily linked: a study on wheat varieties differing in drought resistance under consecutive water salinity stresses. J Plant Physiol 145:168–174
  • Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicol Environ Saf 60:324–349
  • Park S-H, Wilson JD, Seabourn BW (2009) Starch granule size distribution of hard red winter and hard red spring wheat: its effects on mixing and breadmaking quality. J Cereal Sci 49:98–105
  • Passioura JB, Munns R (2000) Rapid environmental changes that affect leaf water status induce transient surges or pauses in leaf expansion rate. Aust J Plant Physiol 27:941–948
  • Quartacci MF, Glisic O, Steranovic B, Navarri-Izzo F (2002) Plasma membrane lipids in the resurrection plants Ramonda serbica following dehydratation and rehydratation. J Exp Bot 53:2159–2166
  • Romero-Aranda MR, Jurado O, Cuartero J (2006) Silicon alleviates the deleterious salt effect on tomato plant growth by improving plant water status. J Plant Physiol 163:847–855
  • Rossi S, Simard S, Rathgeber CBK, Deslauriers A, Zan C (2009) Effects of a 20-day-long dry period on cambial and apical meristem growth in Abies balsamea seedlings. Trees 23:85–93
  • Schar C, Vidale PL, Luthi D, Frei C, Haberli C, Liniger MA, Appenenzeller C (2004) The role of increasing temperature variability in European summer heatwaves. Nature 427:332–336
  • Slama I, Ghaya T, Savouré A, Abdelly C (2008) Combined effects of long-term salinity and soil drying on growth, water relations, nutrient status and proline accumulation of Sesuvium portulacastrum. C R Biol 331:442–451
  • Silva EN, Ferreira-Silva SL, Fontenelea AV, Ribeirob RV, Viégasc RA, Silveira JAG (2010) Photosynthetic changes and protective mechanisms against oxidative damage subjected to isolated and combined drought and heat stresses in Jatropha curcas plants. J Plant Physiol 167:1157–1164
  • Sucre B, Suárez N (2011) Effect of salinity and PEG-induced water stress on water status, gas exchange, solute accumulation, and leaf growth in Ipomoea pes-caprae. Environ Exp Bot 70:192–203
  • Tabaei-Aghdaei S, Harrison P, Pearee RS (2000) Expression of dehydration-stress related genes in crown of wheat, grass species having contrasting acclimation to salt, cold and drought. Plant Cell Environ 23:561–571
  • Tuomi I, Gargouri M, Nouairi I, Moschou PN, Salem-Fnayou AB, Mliki A, Zarrouk M, Ghorbel A (2008) Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance. Biol Plant 52: 161–164
  • Tunçtürk M, Tunçtürk R, Yildirim B, Çiftçi V (2011) Effect of salinity stress on plant fresh weight and nutrient composition of some Canola (Brassica napus L.) cultivars. Afr J Biotechnol 10:1827–1832
  • Türkan I, Demiral T (2009) Recent development in understanding salinity tolerance. Environ Exp Bot 67:2–9
  • Walter J, Nagy L, Hein R, Rascher U, Beierkuhnlein C, Willner E, Jentsch A (2011) Do plants remember drought? Hints towards a drought-memory in grasses. Environ Exp Bot 71:34–40
  • Wang W-B, Kim Y-H, Lee H-S, Kim K-Y, Deng X-P, Kwak S-S (2009) Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol Biochem 47:570–577
  • Zembala M, Filek M, Walas S, Mrowiec H, Kornaś A, Mieszalski Z, Hartikainen H (2010) Effect of selenium on macro- and microelement distribution and physiological parameters of rape and wheat seedlings exposed to cadmium stress. Plant Soil 329:457–468


Rekord w opracowaniu

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.