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2012 | 34 | 5 |

Tytuł artykułu

Antioxidative-related enzyme activity in Alyssum markgrafii shoot cultures as affected by nickel level

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Shoot cultures of rare Balkan hyperaccumulating species Alyssum markgrafii were subjected to high nickel concentrations of 1, 3, 5, and 8 mM. The effects of graded nickel concentrations on toxicity, pigments, and several components of plant antioxidative defense system were characterized. Toxic effects of excess nickel were observed through slower growth and biomass decrease, together with increased reactive oxygen species (ROS) production and lipid peroxidation. Nickel exposure decreased chlorophyll a, b, total chlorophyll as well as carotenoid concentration. Addition of sodium benzoate, potent ROS scavenger, showed concentration-dependent disturbing effect on nickel hyperaccumulation, lowering the content of accumulated nickel in A. markgrafii shoots. General reducing power represented by low molecular weight antioxidants and phenols was inversely correlated with nickel concentration. Among the investigated antioxidative enzymes, POD seems to play important role in ROS level regulation upon excessive nickel in medium.

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  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, P.O.Box 23, 11 010 Belgrade, Serbia
  • Institute for Biological Research "Sinisa Stankovic", University of Belgrade, Despota Stefana 142, Belgrade, Serbia
  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, P.O.Box 23, 11 010 Belgrade, Serbia
  • Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, P.O.Box 23, 11 010 Belgrade, Serbia
  • Faculty of Biology, University of Belgrade, Studentski tgr 16, Belgrade, Serbia
  • Institute for Biological Research "Sinisa Stankovic", University of Belgrade, Despota Stefana 142, Belgrade, Serbia
  • Institute for Biological Research "Sinisa Stankovic", University of Belgrade, Despota Stefana 142, Belgrade, Serbia


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