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2013 | 16 | 3 |
Tytuł artykułu

Effects of two water disinfectants (chloramine T and peracetic acid) on the epidermis and gills of Garra rufa used in human ichthyotherapy

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Doctor fish (Garra rufa) have recently been used for aesthetic purposes and as a medical treatment in patients with psoriasis (ichthyotherapy). For this particular kind of human therapy it is essential to guarantee adequate hygienic conditions for both people and fish. The aim of this study was to test two concentrations of water disinfectants, chloramine T and peracetic acid, on Garra rufa to ascertain possible exposure damage to the epidermis and gills. Fish were exposed to 2 mg/l and 10 mg/l of chloramine T and to 15 μl/l and 45 μl/l of peracetic acid in a 40-minute static bath up to six times a day for one week. The epidermis and gills were checked for histological changes and the number of epidermal mucous cells, club cells and taste buds were quantified; mucous cells were also characterized histochemically to detect alterations in mucin production. No mortality or severe histological changes were found in treated or control fish. Cell count showed a significant increase (p<0.05) in mucous cells (mean 49.1 ± 6.7 vs 37.0 ± 13.1 of controls) in animals treated with peracetic acid independently of the dose. Club cell number showed a significant (p<0.05) decrease in fish treated with 2 mg/l of chloramine T (mean 74.3 ± 15.6) and with 45 μl/l of peracetic acid (mean 78.17 ± 10.5) compared to controls (mean 107.0 ± 19.2). Histochemical evaluation of mucous cells did not reveal changes in mucin type in fish exposed to the two disinfectants. The results suggest a good tolerability of Garra rufa to the two disinfectants at the concentrations tested.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
16
Numer
3
Opis fizyczny
p.453-461,fig.,ref.
Twórcy
autor
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia, Bologna, Italy
autor
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia, Bologna, Italy
autor
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia, Bologna, Italy
autor
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia, Bologna, Italy
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia, Bologna, Italy
autor
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia, Bologna, Italy
autor
  • Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano Emilia, Bologna, Italy
Bibliografia
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  • Bullock GL, Herman RL, Waggy C (1991) Hatchery efficacy trials with chloramines-T for control of bacterial gill disease. J Aquat Anim Health 3: 48-50.
  • Cinar K, Venol N, Kuru N (2008) The distribution of taste buds in Garra rufa. Anat Histol Embryol 37: 63-66.
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  • Fletcher TC, Jones R, Reid L (1976) Identification of glycoproteins in goblet cells of epidermis and gill of plaice (Pleuronectes platessa L.), flounder (Platichthys flesus (L.)) and rainbow trout (Salmo gairdneri Richardson). Histochem J 8: 597-608.
  • Gaikowski MP, Densmore CL, Blazer VS (2009) Histopathology of repeated, intermittent exposure of chloramine-T to walleye (Sander vitreum) and (Ictalurus punctalus) channel catfish. Aquaculture 287: 28-34.
  • Grassberger M, Hoch W (2006) Ichthyotherapy as alternative treatment for patients with psoriasis: a pilot study. Evid Based Complement Alternat Med 3: 483-488.
  • Halbgewachs CF, Marchant TA, Kusch RC, Chivers DP (2009) Epidermal club cells and the innate immune system of minnows. Biol J Linn Soc 98: 891-897.
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  • MassDEP (2010) Hydrogen peroxide, peracetic acid and sodium percarbonate. Massachusetts Department of Environmental Protection, Massachusetts Department of Agricultural Resources – Massachusetts Executive Office of Energy and Environmental Affairs Boston, 17 pp http://www.mass.gov/eea/docs/agr/pesticides/aquatic/sodium-carbonate-peroxyhydrate-and-hydrogen-peroxide.pdf
  • McManus JFA, Mowry RW (1960) Staining methods. Histological and histochemical, Paul B. Hoeber Inc. Medical Division of Harper & Brothers, New York.
  • Ozçelik S, Polat HH, Akyol M, Yalçin AN, Ozçelik D, Marufihah M (2000) Kangal hot spring with fish and psoriasis treatment. J Dermatol 27: 386-390.
  • Pergetti M, Davoli D, Gaccioli M, Sansebastiano G, Stecchi R (1999) Esperienza di disinfezione con acido peracetico di un effluente di un depuratore biologico. Biologia Ambientale 1: 19-28.
  • Powell MD, Speare DJ, Wright GM (1995) Morphological changes in rainbow trout (Oncorhynchus mykiss) gill epithelia following repeated intermittent exposure to chloramine-T. Can J Zool 73: 154-165.
  • Powell MD, Haman F, Wright GM, Perry SF (1998) Response of rainbow trout (Oncorhynchus mykiss) to a graded hypoxia following repeated intermittent exposure to chloramine-T. Aquaculture 165: 27-39.
  • Rai AK, Srivastava N, Kumari U, Mittal S, Mittal AK (2012) Histochemical analysis of glycoproteins in the secretory cells in the epidermis of the head skin of Indian Major Carp, Labeo rohita. Tissue Cell 44: 409-417.
  • Sanchez JG, Speare DJ, Johnson GJ (1997) Morphometric and histochemical assessment of the branchial tissue response of rainbow trout, Oncorhynchus mykiss (Walbaum), associated with chloramine-T treatment. J Fish Dis 20: 375-381.
  • Sanchez JG, Speare DJ, Sims DE, Johnson JG (1998) Morphometric assessment of epidermal and mucous-biofilm changes caused by exposure of trout to chloramine-T or formalin treatment. J Comp Pathol 118: 81-87.
  • Saxena M, Kulshrestha SK (1981) Histochemical studies of mucosubstances in the epidermis of a nonscaly teleost Mystus (Mystus) vittatus Bl. (Pisces-Bagridae). Histochemistry 72: 155-160.
  • Srivastava N, Kumari U, Rai AK, Mittal S, Mittal AK (2012) Histochemical analysis of glycoproteins in the gill epithelium of an Indian major carp, Cirrhinus mrigala. Acta Histochem 114: 626-635.
  • Straus DL, Meinelt T, Farmer BD, Mitchell AJ (2012a) Peracetic acid is effective for controlling fungus on channel catfish eggs. J Fish Dis 35: 505-511.
  • Straus DL, Meinelt T, Farmer BD, Beck BH (2012b) Acute toxicity and histopathology of channel catfish fry exposed to peracetic acid. Aquaculture 342-343: 134-138.
  • Sudová E, Straus DL, Wienke A, Meinelt T (2010) Evaluation of continuous 4-day exposure to peracetic acid as a treatment for Ichthyophthirius multifiliis. Parasitol Res 106: 539-542.
  • VV AA (2011) Antisepsi e disinfezione in ambito sanitario e socio-sanitario. Agenzia sanitaria e sociale regionale dell’Emilia-Romagna, Centro Stampa Regionale, Bologna, Italy. http://www.docvadis.it/assistenzadomiciliare/document/assistenzadomiciliare/line–guida–disinfezione/fr/metadata/files/0/file/kdisinfezione.pdf
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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