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2013 | 35 | 01 |

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Ca2plus/Hplus exchange in the plasma membrane of Arabidopsis thaliana leaves

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Języki publikacji



A large number of plant Ca2+/H+ exchangers have been identified in endomembranes, but far fewer have been studied for Ca2+/H+ exchange in plasma membrane so far. To investigate the Ca2+/H+ exchange in plasma membrane here, inside-out plasma membrane vesicles were isolated from Arabidopsis thaliana leaves using aqueous two-phase partitioning method. Ca2+/H+ exchange in plasma membrane vesicles was measured by Ca2+-dependent dissipation of a pre-established pH gradient. The results showed that transport mediated by the Ca2+/H+ exchange was optimal at pH 7.0, and displayed transport specificity for Ca2+ with saturation kinetics at Km = 47 lM. Sulfate and vanadate inhibited pH gradient across vesicles and decreased the Ca2+-dependent transport of H+ out of vesicles significantly. When the electrical potential across plasma membrane was dissipated with valinomycin and potassium, the rate of Ca2+/H+ exchange increased comparing to control without valinomycin effect, suggesting that the Ca2+/H+ exchange generated a membrane potential (interior negative), i.e. that the stoichiometric ratio for the exchange is greater than 2H+:Ca2+. Eosin Y, a Ca2+-ATPase inhibitor, drastically inhibited Ca2+/H+ exchange in plasma membrane as it does for the purified Ca2+-ATPase in proteoliposomes, indicating that measured Ca2+/H+ exchange activity is mainly due to a plasma membrane Ca2+ pump. These suggest that calcium (Ca2+) is transported out of Arabidopsis cells mainly through a Ca2+-ATPase-mediated Ca2+/H+ exchange system that is driven by the proton-motive force from the plasma membrane H+-ATPase.

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Opis fizyczny



  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/Institute of Bioscience and Technology, College of Agriculture, Hainan University, Haikou 570228, China
  • College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/Institute of Bioscience and Technology, College of Agriculture, Hainan University, Haikou 570228, China
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/Institute of Bioscience and Technology, College of Agriculture, Hainan University, Haikou 570228, China
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/Institute of Bioscience and Technology, College of Agriculture, Hainan University, Haikou 570228, China
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/Institute of Bioscience and Technology, College of Agriculture, Hainan University, Haikou 570228, China
  • Institute of Medicinal Biotechnology, Taishan Medical University, Taian 271000, China
  • Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources/Institute of Bioscience and Technology, College of Agriculture, Hainan University, Haikou 570228, China


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