Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers

• Autorzy: Dobrowolska A. , Debska J. , Kozlowska M. , Staczek P.
• Języki publikacji: EN

Abstrakty

Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T. rubrum (CBS 120358), T. mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.

Słowa kluczowe

EN

strain differentiation; Microsporum canis; random amplified polymorphic DNA analysis; dermatophyte; molecular analysis; keratinophilic fungi; fungal infection; human disease

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 2
• Opis fizyczny: p.145-148,fig.,ref.

Twórcy

autor

Dobrowolska A.

• Department of Genetics of Microorganisms, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

autor

Debska J.

autor

Kozlowska M.

autor

Staczek P.

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