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2011 | 60 | 2 |
Tytuł artykułu

Strains differentiation of Microsporum canis by RAPD analysis using (GACA)4 and (ACA)5 primers

Warianty tytułu
Języki publikacji
EN
Abstrakty
Molecular analysis of dermatophytes (based on PCR fingerprinting) revealed high clonal differentiation between the genus and species. Microsporum canis (zoophilic dermatophyte, belonging to genus Microsporum), responsible for most cases of tinea capitis in children, tinea corporis in adults and dermatophytoses in cats, is very unique in comparison with other dermatophytes. Results of most molecular studies show that there is no clonal differentiation within M. canis as distinct from other species. The aim of this study was application of (GACA)4 repetitive primer and (ACA)5 primer for typing of M. canis strains isolated from human and animals in Central Poland. Fungal strains: 32 clinical isolates of M. canis, originated from patients from Central Poland; 11 strains isolated from infected cats (6) and dogs (7), reference strains of M. canis (CBS 113480), T. rubrum (CBS 120358), T. mentagrophytes (CBS 120357) and E. floccosum (CBS 970.95). The genomic DNAs of the strains were used as a template in RAPD reaction. No differentiation was observed for the analyzed M. canis strains using (GACA)4 and (ACA)5 typing.
Wydawca
-
Rocznik
Tom
60
Numer
2
Opis fizyczny
p.145-148,fig.,ref.
Twórcy
  • Department of Genetics of Microorganisms, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland
autor
autor
autor
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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