Determination of canthaxanthin in premixture and compound feed by the liquid chromatography method

• Autorzy: Rubaj J. , Bielecka G. , Korol W. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

The analytical procedure of canthaxanthine quantification in premixes and compound feed by the HPLC method was presented. The principle of this method is based on the hydrolysis of powdered formulation of canthaxanthine with trypsin and pepsin in a water solution of ammonia and its purification in an aluminium oxide filled column. The canthaxantine content is determined by high performance liquid chromatography in normal phase with the usage of a DAD detector. The limit of quantification of this method was 1.0 mg/kg. The coefficient of variation (CV) of canthaxantine quantification results was 3.3% in premixtures and 4.68% in compound feed. The Horrat values (Hor) were 0.78, in premixtures and 0.52, in compound feeds, which proved the satisfactory precision of the applied method. The recovery rate of canthaxantine added as standard solution into premixture was 98.2%, and in case of compound feed was 97.3%.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 4
• Opis fizyczny: p.707-712,fig.,ref.

Twórcy

autor

Rubaj J.

• National Research Institute of Animal Production, National Feed Laboratory, 20-079 Lublin, Poland

autor

Bielecka G.

• National Research Institute of Animal Production, National Feed Laboratory, 20-079 Lublin, Poland

autor

Korol W.

• National Research Institute of Animal Production, National Feed Laboratory, 20-079 Lublin, Poland

autor

Kwiatek K.

• National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

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