The effect of selection for body weight on the activity of lysosomal enzymes in the liver and kidney of mice

• Autorzy: Witek B. , Ochwanowska E. , Baranowska D. , Kolataj A.

Warianty tytułu

PL

Wpływ selekcji w kierunku wzrostu masy ciała na aktywność enzymów lizosomowych w wątrobie wątrobie nerce myszy

• Języki publikacji: EN

Abstrakty

EN

The study was conducted on 20 Swiss line male mice divided into two groups – control (n=10) comprised of unselected animals (group C), and experimental (n=10) comprised of mice selected over 24 generations for high live body weight gain (group S). Beginning from day 42 of life mice of both groups received over a period of 14 days a standard pelleted feed containing 16% protein.After 14 days the animals were killed, a lysosomal fraction of liver and kidney cells was obtained and analysed for the activity of acid phosphatase, lysosomal esterase, lysosomal lipase, β-glucuronidase, β-galactosidase, β-glucosidase, β-N-acetylo-hexosaminidase, leucine aminopeptidase, alanine aminopeptidase and cathepsins D and L. In S mice the activities of a majority of the lysosomal hydrolases examined were found to be higher than in C animals.

PL

Badania przeprowadzono na 20 samcach myszy linii Swiss. Podzielono je na dwie grupy − kontrolną (n=10) obejmującą osobniki nieselekcjonowane oraz grupę doświadczalną (n=10), do której należały osobniki selekcjonowane w kierunku wysokich przyrostów masy ciała. Myszy obu grup przez okres 14 dni żywiono paszą o standardowej, tj. 16% zawartości białka. Po upływie 14 dni zwierzęta uśmiercano i w otrzymanej frakcji lizosomowej komórek wątroby i nerek oznaczano aktywność fosfatazy kwaśnej, sterazy lizosomowej, lipazy lizosomowej, b-glukuronidazy, b-galaktozydazy, b-glukozydazy, b-Nacetylohexozaminidazy,aminopeptydazy leucynowej, aminopeptydazy alaninowej oraz katepsyn D i L.Uzyskane wyniki wskazują, że zastosowana selekcja istotnie zwiększała aktywność większości badanych hydrolaz lizosomowych.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2007
• Tom: 25
• Numer: 2
• Opis fizyczny: p.119-125,fig.,ref.

Twórcy

autor

Witek B.

• Department of Animal Physiology, Institute of Biology, Swietokrzyska Academy, Swietokrzyska 15, 25-406 Kielce, Poland

autor

Ochwanowska E.

• Department of Animal Physiology, Institute of Biology, Swietokrzyska Academy, Swietokrzyska 15, 25-406 Kielce, Poland

autor

Baranowska D.

• Department of Animal Physiology, Institute of Biology, Swietokrzyska Academy, Swietokrzyska 15, 25-406 Kielce, Poland

autor

Kolataj A.

• Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

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